Transformation efficiency refers to the ability of a cell to take up and incorporate exogenous DNA, such as plasmids, during a process called transformation. The efficiency of transformation is typically measured as the number of transformants (cells that have taken up the exogenous DNA) per microgram of DNA added to the cells. A higher transformation efficiency means that more cells are able to take up the DNA, and a lower efficiency means that fewer cells are able to do so.
In molecular biology, transformation efficiency is a crucial parameter, it is used to evaluate the ability of different methods to introduce plasmid DNA into cells and to compare the efficiency of different plasmid, vectors and host cells. This efficiency can be affected by a number of factors, including the method used for introducing the DNA, the type of cell and plasmid used, and the conditions under which the transformation is performed. Therefore, measuring and optimizing transformation efficiency is an important step in many molecular biology applications, including genetic engineering, gene therapy and biotechnology.
and 25 Related for: Transformation efficiency information
Transformationefficiency refers to the ability of a cell to take up and incorporate exogenous DNA, such as plasmids, during a process called transformation...
thermal energy transformation, the efficiency of the energy transformation process can be dramatically improved. Energy transformations in the universe...
Digital transformation (DT) is the process of adoption and implementation of digital technology by an organization in order to create new or modify existing...
higher transformationefficiencies of plasmids. SOC medium can also be used to regenerate Klebsiella oxytoca strains for the improved transformation efficiency...
to the cells and DNA the transformationefficiency of biolistics and electroporation is lower than agrobacterial transformation. Microinjection is where...
the drug thalidomide Thioescaline, a psychoactive drug Transformationefficiency, the efficiency by which cells can take up extracellular DNA and express...
Marburg strain and is widely used in research due to its high transformationefficiency. Bacillus globigii, a closely related but phylogenetically distinct...
United Kingdom. This position was created (as "Minister for Efficiency and Transformation") by Prime Minister Boris Johnson in February 2020 as a renaming...
increasing its transformationefficiency. Along with higher transformationefficiency, pGreen has been engineered to ensure transformation integrity. Both...
Energy conversion efficiency (η) is the ratio between the useful output of an energy conversion machine and the input, in energy terms. The input, as well...
normally kill it or prevent its growth. In a transformation reaction, depending on the transformationefficiency, only one in several million to billion cells...
(also called ′sector transformation') entails the adoption of new technologies and management practices that increase the efficiency of production. It can...
internalization of cost-effective energy efficiency as a matter of standard practice. Market transformation has rapidly become the objective of many privately...
councillors from the DA. The single councillor of the African TransformationEfficiency and Affirmative Movement crossed to the Liberal Party. The following...
partly dependent on transformationefficiency. As Ohta wrote, "the turbine generator and the electric motor have nearly the same efficiency, therefore we cannot...
cassette into the plastid. Whilst the transformationefficiency is lower than in agrobacterial mediated transformation, which is also common in plant genetic...
chloride solution (CaCl2). Transformation using electroporation was developed in the late 1980s, increasing the efficiency and bacterial range. In 1907...
oligonucleotides lead to increased transformationefficiency. Fully complementary oligonucleotides pairs lead to 5–10 fold increase in efficiency compared to single oligonucleotides...
size for these three in vivo display systems is limited by the transformationefficiency of each organism. For example, the library size for phage and...
Efficient energy use, or energy efficiency, is the process of reducing the amount of energy required to provide products and services. There are many technologies...
of energy transformation. When a measure of the maximal rate of energy transformation is included in the measure of second law efficiency it is known...
This compound also allows higher transformationefficiency in plants, as shown in A. tumefaciens-mediated transformation procedures, and so is of importance...
nature so that cells can become permeable to DNA. Although the efficiency of transformation is often poor, this process is relatively simple and quick to...
Such systems are generally only limited in throughput by the transformationefficiency of cells. They are also less expensive and labour-intensive than...
Global Information