A shuttle vector is a vector (usually a plasmid) constructed so that it can propagate in two different host species.[1] Therefore, DNA inserted into a shuttle vector can be tested or manipulated in two different cell types. The main advantage of these vectors is they can be manipulated in E. coli, then used in a system which is more difficult or slower to use (e.g. yeast).
Shuttle vectors include plasmids that can propagate in eukaryotes and prokaryotes (e.g. both Saccharomyces cerevisiae and Escherichia coli) or in different species of bacteria (e.g. both E. coli and Rhodococcus erythropolis). There are also adenovirus shuttle vectors, which can propagate in E. coli and mammals.
Shuttle vectors are frequently used to quickly make multiple copies of the gene in E. coli (amplification). They can also be used for in vitro experiments and modifications (e.g. mutagenesis, PCR).
One of the most common types of shuttle vectors is the yeast shuttle vector.[2] Almost all commonly used S. cerevisiae vectors are shuttle vectors. Yeast shuttle vectors have components that allow for replication and selection in both E. coli cells and yeast cells. The E. coli component of a yeast shuttle vector includes an origin of replication and a selectable marker, e.g. antibiotic resistance, beta lactamase, beta galactosidase. The yeast component of a yeast shuttle vector includes an autonomously replicating sequence (ARS), a yeast centromere (CEN), and a yeast selectable marker (e.g. URA3, a gene that encodes an enzyme for uracil synthesis, Lodish et al. 2007).
^"vector AND mcb[book] - Books - NCBI". www.ncbi.nlm.nih.gov.
^"Biochemistry & Biophysics - University of Rochester Medical Center".
A shuttlevector is a vector (usually a plasmid) constructed so that it can propagate in two different host species. Therefore, DNA inserted into a shuttle...
another cell Cloning vector, a small piece of DNA into which a foreign DNA fragment can be inserted for cloning purposes Shuttlevector, a plasmid constructed...
international relations tactic Shuttlevector, vector that shuttles between species, biochemistry, genetics 20-yard shuttle, a test performed by American...
Some vectors contain two selectable markers, for example the plasmid pACYC177 has both ampicillin and kanamycin resistance gene. Shuttlevector which...
to be maintained in another organism, and these vectors are called shuttlevectors. An expression vector must have elements necessary for gene expression...
engineering. For example, a research team has actually constructed a PAC shuttlevector that creates a library representing two-fold coverage of the human genome...
of the genus Agrobacterium, such as A. tumefaciens. The binary vector is a shuttlevector, so-called because it is able to replicate in multiple hosts (e...
The Space Shuttle is a retired, partially reusable low Earth orbital spacecraft system operated from 1981 to 2011 by the U.S. National Aeronautics and...
The Space Shuttle Solid Rocket Booster (SRB) was the first solid-propellant rocket to be used for primary propulsion on a vehicle used for human spaceflight...
DNA from bacteria to plants), then a multiple host range vector (also termed shuttlevector) may be selected. In practice, however, specialized molecular...
and the Space Shuttle used gimbaled engines. A later method developed for solid propellant ballistic missiles achieves thrust vectoring by deflecting...
"Mutagenicity of DNA adducts derived from ethylene oxide exposure in the pSP189 shuttlevector replicated in human Ad293 cells". Mutat. Res. 678 (2): 129–37. doi:10...
Assault shuttle previously captured by the Vector tractor beam. Solo and Chewbacca make their way to the bridge in an attempt to either fly the Vector, or...
had profound biotechnological implications for the development of shuttlevectors that can be propagated in bacterial, yeast and mammalian cells. Most...
and applications of a highly transmissible murine retrovirus shuttlevector. This Vector is used for entering a cell in the humans cell body". Cell. 37...
Transgenic mouse assay using a mouse strain infected with a viral shuttlevector is another method for testing mutagens. Animals are first treated with...
"Mutagenicity of DNA adducts derived from ethylene oxide exposure in the pSP189 shuttlevector replicated in human Ad293 cells". Mutat. Res. 678 (2): 129–37. doi:10...
The Shuttle Landing Facility (SLF), also known as Launch and Landing Facility (LLF) (IATA: QQS, ICAO: KTTS, FAA LID: TTS), is an airport located on Merritt...
by the lack of genetic tools. However, practical Nocardia–E. coli shuttlevectors have been developed recently. The genera Nocardia and Rhodococcus have...
Gimbaled thrust is the system of thrust vectoring used in most rockets, including the Space Shuttle, the Saturn V lunar rockets, and the Falcon 9. In...
"Mutagenicity of DNA adducts derived from ethylene oxide exposure in the pSP189 shuttlevector replicated in human Ad293 cells". Mutat. Res. 678 (2): 129–37. doi:10...
bus tours of the spaceport. The "Space Shuttle Atlantis" exhibit contains the Atlantis orbiter and the Shuttle Launch Experience, a simulated ride into...
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