Virus or plasmid designed for gene expression in cells
A bacterial expression vector for expressing green fluorescent protein from the T7 promoter.
An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.
The vector is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector.[1] The goal of a well-designed expression vector is the efficient production of protein, and this may be achieved by the production of significant amount of stable messenger RNA, which can then be translated into protein. The expression of a protein may be tightly controlled, and the protein is only produced in significant quantity when necessary through the use of an inducer, in some systems however the protein may be expressed constitutively. Escherichia coli is commonly used as the host for protein production, but other cell types may also be used. An example of the use of expression vector is the production of insulin, which is used for medical treatments of diabetes.
An expressionvector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. The vector is used...
an expressionvector. The target DNA may be inserted into a site that is under the control of a particular promoter necessary for the expression of the...
has generated over $55 million for the lab. An expressionvector, most commonly the pET expressionvector, is engineered to integrate two essential components:...
therapies were viral vector-based. Compared to traditional vaccines, the intracellular antigen expression enabled by viral vector vaccines offers robust...
found in a variety of vectors, including cloning vectors to increase the number of copies of target DNA, and in expressionvectors to create a protein product...
harvest a particular protein from the recombinant organism, then an expressionvector is chosen that contains appropriate signals for transcription and...
However, unlike "stable expression," the foreign DNA does not fuse with the host cell DNA, resulting in the inevitable loss of the vector after several cell...
promoter frequently used to drive high levels of gene expression in mammalian expressionvectors. CAG promoter was constructed in the lab of Dr Jun-ichi...
RNA (shRNA/Hairpin Vector) is an artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA interference...
A viral vector vaccine is a vaccine that uses a viral vector to deliver genetic material (DNA) that can be transcribed by the recipient's host cells as...
physics, and engineering, a Euclidean vector or simply a vector (sometimes called a geometric vector or spatial vector) is a geometric object that has magnitude...
"Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector". Gene Therapy. 15 (24):...
Microsoft Expression Design was Microsoft's commercial professional illustration vector and raster graphic design tool for web images based on Creature...
used expression systems are cell-based and may be defined as the "combination of an expressionvector, its cloned DNA, and the host for the vector that...
expressionvector. By fusing a target protein to a fluorescent reporter, the protein's behavior, including its cellular localization and expression level...
Expression cloning is a technique in DNA cloning that uses expressionvectors to generate a library of clones, with each clone expressing one protein...
represent vectors and E is the electric field vector; H is the magnetic field's auxiliary field vector or magnetizing field. This expression is often called...
In vector calculus, divergence is a vector operator that operates on a vector field, producing a scalar field giving the quantity of the vector field's...
protein is not necessarily produced. Expression of foreign proteins requires the use of specialized expressionvectors and often necessitates significant...
An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell. In each...
expression templates : An example implementation of expression templates looks like the following. A base class VecExpression represents any vector-valued...
column vectors.) The transpose (indicated by T) of any row vector is a column vector, and the transpose of any column vector is a row vector: [ x 1 x...
In mathematics and physics, a vector space (also called a linear space) is a set whose elements, often called vectors, may be added together and multiplied...
recipient cell to express the cDNA as a protein (i.e., it is put in an expressionvector). Methods for transferring foreign genetic material into a recipient...
site, and a selectable marker. Certain vectors can carry additional regulatory elements based on the expression system involved. DNA constructs can be...
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