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Marker gene information


In biology, a marker gene may have several meanings. In nuclear biology and molecular biology, a marker gene is a gene used to determine if a nucleic acid sequence has been successfully inserted into an organism's DNA. In particular, there are two sub-types of these marker genes: a selectable marker and a marker for screening. In metagenomics and phylogenetics, a marker gene is an orthologous gene group which can be used to delineate between taxonomic lineages.[1]

  1. ^ Ren R, Sun Y, Zhao Y, Geiser D, Ma H, Zhou X (2016). "Phylogenetic Resolution of Deep Eukaryotic and Fungal Relationships Using Highly Conserved Low-Copy Nuclear Genes". Genome Biol Evol. 8 (9): 2683–701. doi:10.1093/gbe/evw196. PMC 5631032. PMID 27604879.

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Marker gene

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In biology, a marker gene may have several meanings. In nuclear biology and molecular biology, a marker gene is a gene used to determine if a nucleic acid...

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Genetic marker

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A genetic marker is a gene or DNA sequence with a known location on a chromosome that can be used to identify individuals or species. It can be described...

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Gene gun

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cell size), and the device was used to deliver particles coated with a marker gene which would relay a signal if proper insertion of the DNA transcript...

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Selectable marker

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Selectable markers are a genes introduced into a cell, especially a bacterium or to cells in culture, that confer a traits suitable for artificial selection...

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Amplicon sequence variant

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inferred single DNA sequences recovered from a high-throughput analysis of marker genes. Because these analyses, also called "amplicon reads," are created following...

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Genetic engineering techniques

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sequencing. Added genes are often accompanied by promoter and terminator regions as well as a selectable marker gene. The added gene may itself be modified...

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URA3

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a gene on chromosome V in Saccharomyces cerevisiae (yeast). Its systematic name is YEL021W. URA3 is often used in yeast research as a "marker gene", that...

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Tumor marker

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proteins, carbohydrates, receptors and gene products. Proteins include hormones and enzymes. To detect enzyme tumor markers enzyme activity is measured. They...

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Cloning vector

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Another kind of selectable marker allows for the positive selection of plasmid with cloned gene. This may involve the use of a gene lethal to the host cells...

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Gene mapping

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between different sites within a gene. The essence of all genome mapping is to place a collection of molecular markers onto their respective positions...

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Subcloning

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desired plasmids to be harvested), a marker gene is used in the destination vector for selection. Typical marker genes are for antibiotic resistance or nutrient...

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Human microbiome

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recently, shotgun metagenomic studies. The former focuses on specific known marker genes and is primarily informative taxonomically, while the latter is an entire...

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QIIME

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originally developed for analysis of high-throughput microbiome marker gene (e.g., 16S or 18S rRNA genes) amplicon sequencing data. There have been two major versions...

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Yeast artificial chromosome

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from S. cerevisiae. Additionally, selectable marker genes, such as antibiotic resistance and a visible marker, are utilized to select transformed yeast cells...

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Abcam

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labelling capabilities by acquiring Marker Gene Technologies for an undisclosed amount. The acquisition of Marker Gene Technologies bring expertise in the...

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Operational taxonomic unit

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organisms, grouped by DNA sequence similarity of a specific taxonomic marker gene (originally coined as mOTU; molecular OTU). In other words, OTUs are...

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Molecular cloning

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selectable marker gene encoded by the vector are able to survive. When bacterial cells are used as host organisms, the selectable marker is usually a gene that...

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Gene trapping

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with gene trap vectors whose principal element is a gene trapping cassette consisting of a promoterless reporter gene and/or selectable genetic marker, flanked...

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Knockout mouse

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is changed sufficiently to make the gene inoperable. Usually, the new sequence is also given a marker gene, a gene that normal mice don't have and that...

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PICRUSt

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inference of the functional profile of a microbial community based on marker gene survey along one or more samples. In essence, PICRUSt takes a user supplied...

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Marker

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Look up Marker, marker, or markers in Wiktionary, the free dictionary. The term Marker may refer to: Marker (linguistics), a morpheme that indicates some...

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Metagenomics

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"Metagenomic approaches in microbial ecology: an update on whole-genome and marker gene sequencing analyses". Microbial Genomics. 6 (8). doi:10.1099/mgen.0.000409...

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List of intestinal stem cell marker genes

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The following is a list of intestinal stem cell marker genes, including their name and known function. In the adult intestine, the crypts of Lieberkühn...

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Stem cell marker

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Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. Stem cells can also be identified by functional...

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Genetic engineering

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terminator region, which initiate and end transcription. A selectable marker gene is added, which in most cases confers antibiotic resistance, so researchers...

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Biomarker

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(journal) Endophenotype Imaging biomarker Marker gene Molecular marker Saliva testing Sponge biomarkers Tumor marker Hirsch MS, Watkins J (May 2020). "A Comprehensive...

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Polly and Molly

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and 7LL12. These animals were transfected but contained a marker gene not the cloned gene of interest. These were named "Holly" and "Olly". Two more...

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Transfection

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transfected gene actually remain in the genome of the cell and its daughter cells, a stable transfection must occur. To accomplish this, a marker gene is co-transfected...

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