Illumina dye sequencing is a technique used to determine the series of base pairs in DNA, also known as DNA sequencing. The reversible terminated chemistry concept was invented by Bruno Canard and Simon Sarfati at the Pasteur Institute in Paris.[1][2] It was developed by Shankar Balasubramanian and David Klenerman of Cambridge University,[3] who subsequently founded Solexa, a company later acquired by Illumina. This sequencing method is based on reversible dye-terminators that enable the identification of single nucleotides as they are washed over DNA strands. It can also be used for whole-genome and region sequencing, transcriptome analysis, metagenomics, small RNA discovery, methylation profiling, and genome-wide protein-nucleic acid interaction analysis.[4][5]
The DNA attaches to the flow cell via complementary sequences. The strand bends over and attaches to a second oligo forming a bridge. A polymerase synthesizes the reverse strand. The two strands release and straighten. Each forms a new bridge (bridge amplification). The result is a cluster of DNA forward and reverse strand clones.
^CA 2158975, Canard, Bruno & Sarfati, Simon, "Novel derivatives usable for the sequencing of nucleic acids", published 1994-10-13, assigned to Pasteur Institute
Illuminadyesequencing is a technique used to determine the series of base pairs in DNA, also known as DNA sequencing. The reversible terminated chemistry...
for validation of deep sequencing results. It still has the advantage over short-read sequencing technologies (like Illumina) in that it can produce...
high-throughput sequencing products included Illumina, Qiagen and ThermoFisher Scientific. SMRT sequencing is based on the sequencing by synthesis approach...
for the Solexa and Illumina machines. Sequencing by reversible terminator chemistry can be a four-colour cycle such as used by Illumina/Solexa, or a one-colour...
terminators instead, commonly called 'dye terminator sequencing'. The major advantage of this approach is the complete sequencing set can be performed in a single...
method of next-generation DNA sequencing which is commonly known today as the Solexa sequencing or Illuminadyesequencing. The method is based on the detection...
ultra-high-throughput sequencing, as many as 500,000 sequencing-by-synthesis operations may be run in parallel. The Illuminadyesequencing method is based...
the 454, SOLiD and Illumina DNA sequencing platforms. Next generation sequencing machines have increased the rate of DNA sequencing substantially, as compared...
mRNA capture and reverse transcription, sequencing libraries are prepared and analyzed with Illuminadyesequencing. The spatial barcode present within each...
The Illumina Methylation Assay using the Infinium I platform uses 'BeadChip' technology[clarification needed] to generate a comprehensive genome-wide profiling...
directly attached to a magnetic bead through an adapter, while for Illuminasequencing, the RNA fragments are firstly ligated to the adapters and then attached...
sell Manteia's colony DNA sequencing technology to UK based Solexa Ltd, now part of Illumina (company). Illuminadyesequencing Pascal Mayer, Francisco...
Bisulfite sequencing (also known as bisulphite sequencing) is the use of bisulfite treatment of DNA before routine sequencing to determine the pattern...
genome was completely sequenced at McDonnell Genome Institute using Illuminadyesequencing. The genome is an exceptionally reduced genome, where the genetic...
how the structure of RNA impacted the fate of a cell. She uses illuminadyesequencing for high throughput SHAPE probing. She develops computer simulations...
After the whole genome is sequenced using a high throughput sequencing method, such as Illumina HiSeq, personalized analysis of rearranged ends (PARE) is...
requires two separate single-dye hybridizations. Several popular single-channel systems are the Affymetrix "Gene Chip", Illumina "Bead Chip", Agilent single-channel...
S2CID 11115152. Pederson ER, Warshan D, Rasmussen U (September 2019). "Genome Sequencing of Pleurozium schreberi: The Assembled and Annotated Draft Genome of a...
In 2014, the complete genome of H. larsenii was sequenced using Illuminadyesequencing HiSeq 2000 by Iain Anderson as part of the Archaeal Tree of Life...
shows, such a design was first demonstrated (and later commercialized by Illumina) using functionalized beads placed randomly in the wells of an etched fiber...
achieved in APEX methods, which translates into lower output per run. Illumina Incorporated's Infinium assay is an example of a whole-genome genotyping...
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