In biochemistry and molecular biology, saline-sodium citrate (SSC) buffer is used as a hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting. 20X SSC may be used to prevent drying of agarose gels during a vacuum transfer.
A 20X stock solution consists of 3 M sodium chloride and 300 mM trisodium citrate (adjusted to pH 7.0 with HCl).
biochemistry and molecular biology, saline-sodium citrate (SSC) buffer is used as a hybridization buffer, to control stringency for washing steps in protocols...
designation for at-risk wildlife in the state of California SSCbuffer, a saline sodium citrate buffer commonly used in molecular biology and organic chemistry...
membrane. If transferring by suction, 20X SSCbuffer is used to ensure a seal and prevent drying of the gel. Buffer transfer by capillary action from a region...
northern blot membranes are pre-hybridized in Denhardt's solution with SSCbuffer and labeled cDNA probes are denatured at 100 °C and added to the pre-hybridization...
present are the citrate ion and mono-hydrogen citrate ion. The SSC 20X hybridization buffer is an example in common use. Tables compiled for biochemical...
89.6002000°W / 30.3627667; -89.6002000 The John C. Stennis Space Center (SSC) is a NASA rocket testing facility in Hancock County, Mississippi, United...
hills and has a core area of 89.52 km2 (34.56 sq mi). It is surrounded by a buffer zone of 148 km2 (57 sq mi). This national park has some rare species of...
initialize screen modes and gain direct access to a card's linear frame buffer, because these tasks would otherwise require handling many hundreds of proprietary...
actively processed, computer memory serves as mass storage cache and write buffer to improve both reading and writing performance. Operating systems borrow...
Cats: status survey and conservation action plan. Gland, Switzerland: IUCN/SSC Cat Specialist Group. pp. 55–65. O’Brien, T. G.; Kinnard, M. F. & Wibisono...
the bear's thick skin and layers of fat as a buffer against most physical attacks, sometimes buffering to some extent even against firearms. According...
cheetahs at Kuno National Park with a core zone of 748 km2 (289 sq mi) and a buffer zone of 487 km2 (188 sq mi). Increasing cheetah population leads to the...
classification under the wolf debated. In 2019, a workshop hosted by the IUCN/SSC Canid Specialist Group considered the New Guinea singing dog and the dingo...
The State Security Council (SSC) was formed in South Africa in 1972 to advise the government on the country's national policy and strategy concerning...
Sanctuary of Atotonilco, has a core zone of .75 hectares surrounded by a buffer zone of about 4.5 hectares. Before the arrival of the Spanish in the early...
carbon dioxide, the latter of which acidifies the blood via the bicarbonate buffer system. The resulting acidity causes the hemoglobin of the blood to lose...
estimations the species would actually qualify as "near threatened". IUCN SSC Antelope Specialist Group (2 August 2016). "Damaliscus lunatus ssp. jimela"...
2015, pp. 44–45. Levi, Werner (December 1959), "Bhutan and Sikkim: Two Buffer States", The World Today, 15 (2): 492–500, JSTOR 40393115 du Plessix Gray...
the semiconductor industry. Shift registers, processor registers, data buffers and other small digital registers that have no memory address decoding...
Talukdar, B.K. (2008). Report on the regional meeting for India and Nepal IUCN/SSC Asian Rhino Species Group (AsRSG), March 5–7, 2007 Kaziranga National Park...
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