Agarose gelTray with a stack consisting top down of a weight, paper towels, membrane of nitrocellulose or nylon, gel, salt solution and a slab of glass.Southern blot membrane after hybridization and rinsing.Southern blot agarose gel under ultraviolet illumination.Southern blot autoradiogram.
Southern blot is a method used for detection and quantification of a specific DNA sequence in DNA samples. This method is used in molecular biology. Briefly, purified DNA from a biological sample (such as blood or tissue) is digested with restriction enzymes, and the resulting DNA fragments are separated by using an electric current to move them through a sieve-like gel or matrix, which allows smaller fragments to move faster than larger fragments. The DNA fragments are transferred out of the gel or matrix onto a solid membrane, which is then exposed to a DNA probe labeled with a radioactive, fluorescent, or chemical tag. The tag allows any DNA fragments containing complementary sequences with the DNA probe sequence to be visualized within the Southern blot.[1]
The Southern blotting combines the transfer of electrophoresis-separated DNA fragments to a filter membrane in a process called blotting, and the subsequent fragment detection by probe hybridization.[2]
The method is named after the British biologist Edwin Southern, who first published it in 1975.[3] Other blotting methods (i.e., western blot,[4] northern blot, eastern blot, southwestern blot) that employ similar principles, but using RNA or protein, have later been named for compass directions as a sort of pun from Southern's name. As the label is eponymous, Southern is capitalized, as is conventional of proper nouns. The names for other blotting methods may follow this convention, by analogy.[5]
^"Talking Glossary of Genetic Terms | NHGRI". www.genome.gov. National Institutes of Health. Retrieved 24 January 2023. This article incorporates text from this source, which is in the public domain.
^"Southern Blot".
^Southern, Edwin Mellor (5 November 1975). "Detection of specific sequences among DNA fragments separated by gel electrophoresis". Journal of Molecular Biology. 98 (3): 503–517. doi:10.1016/S0022-2836(75)80083-0. ISSN 0022-2836. PMID 1195397. S2CID 20126741.
^Towbin; Staehelin, T; Gordon, J; et al. (1979). "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications". PNAS. 76 (9): 4350–4. Bibcode:1979PNAS...76.4350T. doi:10.1073/pnas.76.9.4350. PMC 411572. PMID 388439.
^Burnette, W. Neal (April 1981). "Western Blotting: Electrophoretic Transfer of Proteins from Sodium Dodecyl Sulfate-Polyacrylamide Gels to Unmodified Nitrocellulose and Radiographic Detection with Antibody and Radioiodinated Protein A". Analytical Biochemistry. 112 (2): 195–203. doi:10.1016/0003-2697(81)90281-5. ISSN 0003-2697. PMID 6266278.
Southernblot is a method used for detection and quantification of a specific DNA sequence in DNA samples. This method is used in molecular biology. Briefly...
name from its similarity to the first blotting technique, the Southernblot, named for biologist Edwin Southern. The major difference is that RNA, rather...
widely known for the invention of the Southernblot, published in 1975 and now a common laboratory procedure. Southern was born in Burnley, Lancashire and...
The eastern blot, or eastern blotting, is a biochemical technique used to analyze protein post-translational modifications including the addition of lipids...
The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics...
by Edwin Southern for the hybridisation of blotted DNA. Patricia Thomas, developer of the RNA blot which then became known as the northern blot, actually...
underlying Southernblotting and Western blotting techniques of which they detect DNA and protein respectively. Similar to other types of blotting, proteins...
as agarose gel electrophoresis and transferred to a membrane via the Southernblot procedure. Hybridization of the membrane to a labeled DNA probe then...
for washing steps in protocols for Southernblotting, in situ hybridization, DNA Microarray or Northern blotting. 20X SSC may be used to prevent drying...
matrix. Southernblot Western blot Northern blot Southwestern blot Eastern blot Gel electrophoresis SDS-PAGE Chromatography Northwestern Blot of Protein-RNA...
A zoo blot or garden blot is a type of Southernblot that demonstrates the similarity between specific, usually protein-coding, DNA sequences of different...
fluorescent protein vector Enzyme assay Protein purification Western blot Northern blotSouthernblot Restriction enzyme Polymerase chain reaction Two-hybrid screening...
range of biochemical methods, such as protein fingerprinting, Southernblot, other blotting procedures, DNA sequencing, and many more. Suspended particles...
discernable fragments. Following the enzyme digestion, a SouthernBlot is performed. SouthernBlots are a size-based separation method that are performed...
fluorescent protein vector Enzyme assay Protein purification Western blot Northern blotSouthernblot Restriction enzyme Polymerase chain reaction Two-hybrid screening...
such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southernblotting for further characterization. Electrophoresis is a process that enables...
nucleic acid simulation software Northern blot Nuclear run-on assay Radioactivity in the life sciences Southernblot Differential centrifugation (sucrose gradient)...
ICSI. In molecular biology, PVP can be used as a blocking agent during Southernblot analysis as a component of Denhardt's buffer. It is also exceptionally...
cannot be used to compare quantitative polymerase chain reaction and Southernblot telomere length measurement technique". International Journal of Epidemiology...
D4Z4 array length, which is more precise and less labor-intensive than southernblot. Molecular combing is also available for assessing D4Z4 array length...
Diagnostic tests include PCR to analyze the number of CGG repeats, Southernblot analysis, and examination of AGG trinucleotides in the FMR1 gene region...
affixed to a microscope slide or a membrane such as a Northern blot or a hybridized slot blot can be placed against x-ray film or phosphor screens to acquire...
eukaryotic cells. One method is the Terminal Restriction Fragment (TRF) southernblot. There is a Web-based Analyser of the Length of Telomeres (WALTER),...
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