Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR). The amplified cDNA copies are then sequenced and, if long enough, should map to a unique genomic region. RACE is commonly followed up by cloning before sequencing of what was originally individual RNA molecules. A more high-throughput alternative which is useful for identification of novel transcript structures, is to sequence the RACE-products by next generation sequencing technologies.
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millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample ofDNA (or a part of it) sufficiently...
Emergency Service, in the US, established in 1952 for wartime use RapidamplificationofcDNAends, a technique in molecular biology RACE (Remote Applications...
Random amplified polymorphic DNA (RAPD), pronounced "rapid", is a type of polymerase chain reaction (PCR), but the segments ofDNA that are amplified are...
also referred to as parallel analysis of RNA ends (PARE), is a modified version of 5'-RapidAmplificationofcDNAEnds (RACE) using high-throughput, deep...
complement each (template) strand. DNA replication occurs during the S-stage of interphase. DNA replication (DNAamplification) can also be performed in vitro...
group in the 5'-end, for instance in protocols for RACE (Rapid AmplificationofcDNAEnds). Shinshi H, Miwa M, Kato K, Noguchi M, Matsushima T, Sugimura...
(August 1993). "Cloning of the 5' mRNA for the 230-kD bullous pemphigoid antigen by rapidamplificationofcDNAends". The Journal of Investigative Dermatology...
complementary DNA (cDNA) is DNA that was reverse transcribed (via reverse transcriptase) from an RNA (e.g., messenger RNA or microRNA). cDNA exists in both...
"Determination of the relative expression levels of rubisco small subunit genes in Arabidopsis by rapidamplificationofcDNAends". Analytical Biochemistry...
combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplificationof specific DNA targets using polymerase...
simplified version of natural rolling circle replication, an isothermal DNAamplification technique, rolling circle amplification was developed. The RCA...
Validation of target cleavage in specific mRNAs is typically performed using a modified version of 5' RapidAmplificationofcDNAEnds with a gene-specific...
further amplification via PCR. After adaptor ligation, cDNA are cleaved using TE to remove them from the beads, leaving only a short "tag" of about 11...
Removal of these inhibitors is crucial to ensure that high quality DNA is available for subsequent analyzing. Amplificationof the extracted DNA is a required...
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