In molecular biology, Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium album proteinase K) is a broad-spectrum serine protease.[2][3][4] The enzyme was discovered in 1974 in extracts of the fungus Parengyodontium album (formerly Engyodontium album or Tritirachium album).[5] Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8 (subtilisin). The molecular weight of Proteinase K is 28,900 daltons (28.9 kDa).
^Betzel C, Singh TP, Visanji M, Peters K, Fittkau S, Saenger W, Wilson KS (July 1993). "Structure of the complex of proteinase K with a substrate analogue hexapeptide inhibitor at 2.2-A resolution". J. Biol. Chem. 268 (21): 15854–8. doi:10.1016/S0021-9258(18)82332-8. PMID 8340410.
^Morihara K, Tsuzuki H (1975). "Specificity of proteinase K from Tritirachium album Limber for synthetic peptides". Agric. Biol. Chem. 39 (7): 1489–1492. doi:10.1271/bbb1961.39.1489.
^Kraus E, Kiltz HH, Femfert UF (February 1976). "The specificity of proteinase K against oxidized insulin B chain". Hoppe-Seyler's Z. Physiol. Chem. 357 (2): 233–7. PMID 943367.
^Jany KD, Lederer G, Mayer B (1986). "Amino acid sequence of proteinase K from the mold Tritirachium album Limber". FEBS Lett. 199 (2): 139–144. doi:10.1016/0014-5793(86)80467-7. S2CID 85577597.
^Ebeling W, Hennrich N, Klockow M, Metz H, Orth HD, Lang H (August 1974). "Proteinase K from Tritirachium album Limber". Eur. J. Biochem. 47 (1): 91–7. doi:10.1111/j.1432-1033.1974.tb03671.x. PMID 4373242.
to the protein's structure or function. PrPC is readily digested by proteinaseK and can be liberated from the cell surface by the enzyme phosphoinositide...
activity or removal of unwanted proteins. For example, proteinaseK, a broad-spectrum proteinase stable in urea and SDS, is often used in the preparation...
carbohydrates, proteins, and extracellular DNA. Detachment assays with NaIO4, proteinaseK, or DNase result in 38%, 98%, and 100% detachment, respectively. The...
fluorescence or brightfield microscope. permeabilization of cells with proteinaseK to open cell membranes (around 25 minutes, not needed for tissue sections...
RNA unusable. To remove DNA and proteins, enzymes such as DNase and ProteinaseK are used for degradation. Importantly, RNA integrity is maintained by...
used to break open cells and release the DNA. Protease enzymes, such as ProteinaseK, which are used to digest proteins that may be binding to the DNA. Phenol...
A protease (also called a peptidase, proteinase, or proteolytic enzyme) is an enzyme that catalyzes proteolysis, breaking down proteins into smaller polypeptides...
improve image quality in gastroscopy by thinning the mucus in advance. ProteinaseK Teh, Jun-Liang; Shabbir, Asim; Yuen, Soon; So, Jimmy Bok-Yan (2020)....
can also degrade PLA. Enzymes such as pronase and most effectively proteinaseK from Tritirachium album degrade PLA. Four possible end-of-life scenarios...
proteolytic induced epitope retrieval (PIER). In PIER, enzymes including ProteinaseK, Trypsin, and Pepsin have been used to restore antibody binding to its...
(EC 3.4.21.59, ) is the most abundant secretory granule-derived serine proteinase contained in mast cells and has been used as a marker for mast cell activation...
papaya peptidase II, papaya proteinase, papaya proteinase III, papaya proteinase 3, proteinase omega, papaya proteinase A, chymopapain S, Pp) is an enzyme...
biocon.2014.11.019. Tsuji, Satsuki (2016). "Effects of water pH and proteinaseK treatment on the yield of environmental DNA from water samples". Limnology...
protein–DNA cross-link is reversed and proteins are removed by digestion with proteinaseK. An epitope-tagged version of the protein of interest, or in vivo biotinylation...
Sreekumar K, Purandare U, Lo CF, Huang Y, et al. (December 2003). "Functional characterization of Narc 1, a novel proteinase related to proteinaseK". Archives...
(a protease) cleaves VPg from membrane-bound 3AB. Studies that used proteinaseK to cleave VPg from the viral genome discovered that calicivirus vesicular...
were now subject to cleavage by proteinaseK. The non-OmpA peptides inserted were therefore a target of proteinaseK. Insertion of the foreign peptides...
enzymes involved in microbial plastic biodegradation include lipase, proteinaseK, pronase, and hydrogenase, among others. The efficacy of these enzymes...
cell culture. This can be done by standard extraction methods such as ProteinaseK digestion followed by ethanol precipitation or by other commercially...
antibodies, and unbound DNA is removed in the supernatant. To purify the DNA, proteinaseK is added to digest the antibodies and release the DNA, which can be collected...
PMID 22016395. Oda K, Sugitani M, Fukuhara K, Murao S (March 1987). "Purification and properties of a pepstatin-insensitive carboxyl proteinase from a gram-negative...
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