Global Information Lookup Global Information

Antigen retrieval information


Tissues that have been preserved with formaldehyde, a highly reactive compound, contain a variety of chemical modifications that can reduce the detectability of proteins in biomedical procedures such as immunohistochemistry. Antigen retrieval is an approach to reducing or eliminating these chemical modifications. The two primary methods of antigen retrieval are heat-mediated epitope retrieval (HIER) and proteolytic induced epitope retrieval (PIER).

and 25 Related for: Antigen retrieval information

Request time (Page generated in 0.8573 seconds.)

Antigen retrieval

Last Update:

immunohistochemistry. Antigen retrieval is an approach to reducing or eliminating these chemical modifications. The two primary methods of antigen retrieval are heat-mediated...

Word Count : 419

Immunohistochemistry

Last Update:

tissue for IHC, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody...

Word Count : 3341

Immunostaining

Last Update:

sections.[citation needed] The detection of many antigens can be dramatically improved by antigen retrieval methods that act by breaking some of the protein...

Word Count : 1287

Immunofluorescence

Last Update:

PMID 19063604. Çelik S (January 2015). "Understanding the complexity of antigen retrieval of DNA methylation for immunofluorescence-based measurement and an...

Word Count : 2055

Adult neurogenesis

Last Update:

advocating for the presence of adult neurogenesis have focused on how dual antigen retrieval finds that DCX antibodies are staining many cells within the adult...

Word Count : 12661

Reverse phase protein lysate microarray

Last Update:

experiments. A problem that is encountered with tissue microarrays is antigen retrieval and the inherent subjectivity of immunohistochemistry. Antibodies...

Word Count : 2925

Assay

Last Update:

manipulations e.g. spinning for separation, aliquoting if necessary, storage, retrieval, pipetting, aspiration, etc.). Analytes are generally tested in high-throughput...

Word Count : 3322

Axel Behrens

Last Update:

Miguel-Aliaga, Irene; van Rheenen, Jacco; Behrens, Axel (2020). "Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH". Nature...

Word Count : 868

Toxic shock syndrome

Last Update:

professional antigen presenting cells, with T-cell receptors, on T cells. In typical T-cell recognition, an antigen is taken up by an antigen-presenting...

Word Count : 3419

Positive and negative predictive values

Last Update:

positive predictive value, the two are numerically equal. In information retrieval, the PPV statistic is often called the precision. The positive predictive...

Word Count : 2345

Recognition

Last Update:

Recognition (sociology), a public acknowledgement of person's status or merits Antigen recognition, in immunology Intra-species recognition, the recognition of...

Word Count : 514

CDR

Last Update:

telecommunications, related to the committed information rate Crash Data Retrieval, a tool for imaging or downloading data from an Event data recorder CD-R...

Word Count : 706

Trichomonas vaginalis

Last Update:

culture, with a sensitivity range of 75–95%. Newer methods, such as rapid antigen testing and transcription-mediated amplification, have even greater sensitivity...

Word Count : 3114

Leishmaniasis

Last Update:

diagnosis are developed, including enzyme-linked immunosorbent assay, antigen-coated dipsticks, and direct agglutination test. Although these tests are...

Word Count : 4946

Biotinylation

Last Update:

avidin or streptavidin has a binding affinity that is higher than most antigen and antibody bonds and approaches the strength of a covalent bond. This...

Word Count : 2904

Legionella pneumophila

Last Update:

the outer leaflet of the outer cell membrane. The bases for the somatic antigen specificity of this organism are located on the side chains of its cell...

Word Count : 4498

Mary Higby Schweitzer

Last Update:

Tyrannosaurus skeleton in the Hell Creek Formation in Montana. After a two-year retrieval process, Jack Horner, director of the Museum, gave the femur bone to Schweitzer...

Word Count : 1221

Preimplantation genetic diagnosis

Last Update:

microscopy as well as profiling of RNA and protein expression. Human leukocyte antigen (HLA) typing of embryos, so that the child's HLA matches a sick sibling...

Word Count : 14457

2023 in science

Last Update:

are reported: non-human vaccinated primates-tested pGal–antigen therapy for suppressing antigen-specific immune responses and against autoimmune diseases...

Word Count : 44436

Calnexin

Last Update:

transporter associated with antigen processing (TAP) complex. This association prepares the MHC class I for binding an antigen for presentation on the cell...

Word Count : 2214

Adoptive cell transfer

Last Update:

a T-cell receptor (TCR) that recognized the MART-1 melanoma-melanocyte antigen, mediated tumor regression. In 2010 administration of lymphocytes genetically...

Word Count : 4028

Computational immunology

Last Update:

transport and HLA class I antigen presentation. TAP is a transmembrane protein responsible for the transport of antigenic peptides into the endoplasmic...

Word Count : 4344

Cancer biomarker

Last Update:

Cancer), KIT (gastrointestinal stromal tumor), PSA (prostate specific antigen) (prostate cancer), S100 (melanoma), and many others. Mutant proteins themselves...

Word Count : 4969

Epigenetics

Last Update:

for hemimethylated DNA and interacts with the proliferating cell nuclear antigen (PCNA). By preferentially modifying hemimethylated DNA, DNMT1 transfers...

Word Count : 18659

Vasectomy

Last Update:

Shridharani A, Sandlow JI (2010). "Vasectomy reversal versus IVF with sperm retrieval: which is better?". Current Opinion in Urology. 20 (6): 503–509. doi:10...

Word Count : 6711

PDF Search Engine © AllGlobal.net