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Phenol extraction information


Phenol extraction is a laboratory technique that purifies nucleic acid samples using a phenol solution. Phenol is common reagent in extraction because its properties allow for effective nucleic acid extraction, particularly as it strongly denatures proteins, it is a nucleic acid preservative, and it is immiscible in water.

It may also refer to the process of extracting and isolating phenols from raw materials such as coal tar. These purified phenols are used in many industrial and medical compounds and are used as precursors in some synthesis reactions.

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Phenol extraction

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Phenol extraction is a laboratory technique that purifies nucleic acid samples using a phenol solution. Phenol is common reagent in extraction because...

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Trizol

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guanidinium thiocyanate-phenol-chloroform extraction. The use of TRIzol can result in DNA yields comparable to other extraction methods, and it leads to...

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DNA extraction

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concentration. The specific method used to extract the DNA, such as phenol-chloroform extraction, alcohol precipitation, or silica-based purification. For the...

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Phenolic content in wine

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The phenolic content in wine refers to the phenolic compounds—natural phenol and polyphenols—in wine, which include a large group of several hundred chemical...

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Phenol

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Phenol (also known as carbolic acid, phenolic acid, or benzenol) is an aromatic organic compound with the molecular formula C6H5OH. It is a white crystalline...

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RNA extraction

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thiocyanate-phenol-chloroform extraction. The filter paper based lysis and elution method features high throughput capacity. RNA extraction in liquid nitrogen...

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Naturally occurring phenols

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biochemistry, naturally occurring phenols are natural products containing at least one phenol functional group. Phenolic compounds are produced by plants...

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Polyphenol

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Polyphenols (/ˌpɒliˈfiːnoʊl, -nɒl/) are a large family of naturally occurring phenols. They are abundant in plants and structurally diverse. Polyphenols include...

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Dow process

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bromine extraction from brine Dow process (magnesium), a method of magnesium extraction from brine Dow process (phenol), a method of phenol production...

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Olive oil extraction

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these problems. Sacrificing part of its extraction capability, it uses less added water thus reducing the phenol washing. The olive paste is separated into...

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Winepress

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submitting them to a strong vacuum. The technique allows for a better extraction of phenolic compounds. Ancient winepress in Israel with the pressing area in...

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Gel extraction

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into a small tube. A butanol extraction removes the ethidium bromide stain, followed by a phenol/chloroform extraction of the cleaned DNA fragment. The...

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Carvacrol

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Carvacrol, or cymophenol, C6H3(CH3)(OH)C3H7, is a monoterpenoid phenol. It has a characteristic pungent, warm odor of oregano. Carvacrol is present in...

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Argan oil

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oil also contains traces of tocopherols (vitamin E), phenols, carotenes, squalene. Some trace phenols in argan oil include caffeic acid, oleuropein, vanillic...

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Restriction map

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supercoiled plasmids will be left in solution. This can be followed by phenol extraction to remove proteins and other molecules. Then the DNA can be subjected...

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Isoamyl alcohol

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chloroform isoamyl alcohol reagent. Isoamyl alcohol is used in a phenol–chloroform extraction mixed with the chloroform to further inhibit RNase activity and...

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Plasmid preparation

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buffer. The basic principle of the phenol-chloroform extraction is that DNA and RNA are relatively insoluble in phenol and chloroform, while other cellular...

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Flash release

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technique used in wine pressing. The technique allows for a better extraction of phenolic compounds and wine polysaccharides. The treatment consists of heating...

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Superheated water

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reagent, and catalyst in industrial and analytical applications, including extraction, chemical reactions and cleaning. All materials change with temperature...

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Cyanidioschyzon

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isolation of DNA from C. merolae. The first is a hot phenol extraction, which is a quick extraction that can be used to isolate DNA suitable for DNA amplification...

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Comparative genomic hybridization

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available slides may be used, but should always be tested first. Standard phenol extraction is used to obtain DNA from test or reference (karyotypically normal...

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Thymol

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2-isopropyl-5-methylphenol, IPMP), C10H14O, is a natural monoterpenoid phenol derivative of p-Cymene, isomeric with carvacrol, found in oil of thyme,...

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Nucleic acid quantitation

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260 nm/280 nm spectrophotometric version. Nucleic acid methods Phenol–chloroform extraction Column purification Protein methods Huss, Volker A.R.; Festl...

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Olive oil

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cultivar, region, altitude, time of harvest, and extraction process. Olive oil contains traces of phenolics (about 0.5%), such as esters of tyrosol, hydroxytyrosol...

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Sonication

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probe system is used for extraction. For instance, this technique was suggested to remove isoflavones from soybeans and phenolic compounds from wheat bran...

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Para tertiary butylphenol formaldehyde resin

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p-tert-butylphenol-formaldehyde resin (PTBP-FR) or 4-(1,1-dimethylethyl) phenol (PTBP Formaldehyde) is a phenol-formaldehyde resin found in commercial adhesives, and in...

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