Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.
A plasmid preparation is a method of DNA extraction and purification for plasmid DNA, it is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.[1][2] Many methods have been developed to purify plasmid DNA from bacteria.[1][3] During the purification procedure, the plasmid DNA is often separated from contaminating proteins and genomic DNA.
These methods invariably involve three steps: growth of the bacterial culture, harvesting and lysis of the bacteria, and purification of the plasmid DNA.[4] Purification of plasmids is central to molecular cloning. A purified plasmid can be used for many standard applications, such as sequencing and transfections into cells.
^ abLi JF, Li L, Sheen J (January 2010). "Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology". Plant Methods. 6 (1): 1. doi:10.1186/1746-4811-6-1. PMC 2829548. PMID 20180960.
^Lezin G, Kosaka Y, Yost HJ, Kuehn MR, Brunelli L (2011). "A one-step miniprep for the isolation of plasmid DNA and lambda phage particles". PLOS ONE. 6 (8): e23457. Bibcode:2011PLoSO...623457L. doi:10.1371/journal.pone.0023457. PMC 3156146. PMID 21858126.
^Bouchard R, et al. (2010). Laboratory Methods in Microbiology. Universal Scientific. pp. 119–126.
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inserted into a plasmid and that these foreign sequences would be carried into bacteria and digested as part of the plasmid. That is, these plasmids could serve...
more compact. In a normal plasmid DNA preparation, multiple forms of DNA may be present. Gel electrophoresis of the plasmids would normally show the negatively...
the ability of a cell to take up and incorporate exogenous DNA, such as plasmids, during a process called transformation. The efficiency of transformation...
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compact. In a normal plasmid DNA preparation, multiple forms of DNA may be present, and gel from the electrophoresis of the plasmids would normally show...
increasingly antibiotic resistant bacteria because it can cure (remove) plasmids containing antimicrobial resistance genes from Gram positive bacteria....
been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. It continues to be one of the most...
be used to titer genomic libraries made with non-viral vectors, such as plasmids and BACs. A test ligation of the library can be used to transform E. coli...
different types of plasmids (e.g., for bacterial, insect and mammalian protein expression). The first step in Gateway cloning is the preparation of a Gateway...
from plasmid DNA by centrifugation of lysed bacteria and pelleting of the membranes (and the attached DNA). Prokaryotic chromosomes and plasmids are,...
immune response. DNA vaccines work by injecting genetically engineered plasmid containing the DNA sequence encoding the antigen(s) against which an immune...
provided by the researcher, often as a genetically engineered fragment or plasmid. During uptake, DNA is transported across the cell membrane(s), and the...
health-care product, insecticides, and dips. The chemicals contained in these preparations, besides harming beneficial organisms, may intentionally or inadvertently...
transfer. This includes insertion sequences, bacteriophages, integrons, plasmids, genomic islands, and transposons. Within LAB, they are responsible for...
molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic...
in sterile Milli-Q water to form a 0.2% gelatin solution. Purified DNA plasmid is then mixed with the gelatin solution, and the final gelatin concentration...
Canadian MRSA isolate "MUP87" and is probably located on a nonconjugative plasmid. Pseudomonic acid inhibits isoleucine—tRNA ligase in bacteria, leading...
biodiversity and ecosystem functioning. Bti strains possess the pBtoxis plasmid which encodes numerous Cry (a δ-endotoxin) and Cyt toxins, including Cry4...
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