Molecular biology technique to type microorganisms
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Multilocus sequence typing (MLST) is a technique in molecular biology for the typing of multiple loci, using DNA sequences of internal fragments of multiple housekeeping genes to characterize isolates of microbial species.
The first MLST scheme to be developed was for Neisseria meningitidis,[1] the causative agent of meningococcal meningitis and septicaemia. Since its introduction for the research of evolutionary history, MLST has been used not only for human pathogens but also for plant pathogens.[2]
^Maiden, MC.; Bygraves, JA.; Feil, E.; Morelli, G.; Russell, JE.; Urwin, R.; Zhang, Q.; Zhou, J.; et al. (Mar 1998). "Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms". Proc Natl Acad Sci U S A. 95 (6): 3140–5. Bibcode:1998PNAS...95.3140M. doi:10.1073/pnas.95.6.3140. PMC 19708. PMID 9501229.
^Sarris, PF; Trantas, EA; Mpalantinaki, E; Ververidis, F; Goumas, DE (2012). "Pseudomonas viridiflava, a multi host plant pathogen with significant genetic variation at the molecular level". PLOS ONE. 7 (4): e36090. Bibcode:2012PLoSO...736090S. doi:10.1371/journal.pone.0036090. PMC 3338640. PMID 22558343.
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