Fosmid information

Fosmids are similar to cosmids but are based on the bacterial F-plasmid. The cloning vector is limited, as a host (usually E. coli) can only contain one fosmid molecule. Fosmids can hold DNA inserts of up to 40 kb in size; often the source of the insert is random genomic DNA. A fosmid library is prepared by extracting the genomic DNA from the target organism and cloning it into the fosmid vector.^[1] The ligation mix is then packaged into phage particles and the DNA is transfected into the bacterial host. Bacterial clones propagate the fosmid library. The low copy number offers higher stability than vectors with relatively higher copy numbers, including cosmids. Fosmids may be useful for constructing stable libraries from complex genomes. Fosmids have high structural stability and have been found to maintain human DNA effectively even after 100 generations of bacterial growth.^[2] Fosmid clones were used to help assess the accuracy of the Public Human Genome Sequence.^[3]

^ Hall BG (May 2004). "Predicting the evolution of antibiotic resistance genes". Nature Reviews. Microbiology. 2 (5): 430–5. doi:10.1038/nrmicro888. PMID 15100696. S2CID 8892046.
^ Shizuya H, Birren B, Kim UJ, Mancino V, Slepak T, Tachiiri Y, Simon M (September 1992). "Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector". Proceedings of the National Academy of Sciences of the United States of America. 89 (18): 8794–7. Bibcode:1992PNAS...89.8794S. doi:10.1073/pnas.89.18.8794. PMC 50007. PMID 1528894.
^ Kim UJ, Shizuya H, de Jong PJ, Birren B, Simon MI (March 1992). "Stable propagation of cosmid sized human DNA inserts in an F factor based vector". Nucleic Acids Research. 20 (5): 1083–5. doi:10.1093/nar/20.5.1083. PMC 312094. PMID 1549470.

[pmid_15100696-1] Hall BG (May 2004). "Predicting the evolution of antibiotic resistance genes". Nature Reviews. Microbiology. 2 (5): 430–5. doi:10.1038/nrmicro888. PMID 15100696. S2CID 8892046.

[Shizuya-2] Shizuya H, Birren B, Kim UJ, Mancino V, Slepak T, Tachiiri Y, Simon M (September 1992). "Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector". Proceedings of the National Academy of Sciences of the United States of America. 89 (18): 8794–7. Bibcode:1992PNAS...89.8794S. doi:10.1073/pnas.89.18.8794. PMC 50007. PMID 1528894.

[pmid_1549470-3] Kim UJ, Shizuya H, de Jong PJ, Birren B, Simon MI (March 1992). "Stable propagation of cosmid sized human DNA inserts in an F factor based vector". Nucleic Acids Research. 20 (5): 1083–5. doi:10.1093/nar/20.5.1083. PMC 312094. PMID 1549470.

Fosmid information

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Fosmid

Eukaryote

Animal

Plant

Bacteria

Mitochondrion

Fungus

Virus

Protist

Cotton

Cell division

HeLa

Cloning vector

Archaea

Plasmid

Transposable element

Viroid

Life

Jumping library

Prion

DNA replication

Biological dark matter

Tandem repeat

Ribozyme

Copy number variation

Plastid

Last universal common ancestor