Fluorescence intensity decay shape microscopy information
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Within the scientific study of a molecule's color, Fluorescence intensity decay shape microscopy (FIDSAM) is a fluorescence microscope technique, which utilizes the time evolution of fluorescence emission after a pulsed excitation to analyse the decay statistics of an excited chromophore.[1] The main application of FIDSAM is the discrimination of unspecific autofluorescent background signal from the target signal of a dedicated chromophore.
^Schleifenbaum, Frank; Elgass, Kirstin; Sackrow, Marcus; Caesar, Katharina; Berendzen, Kenneth; Meixner, Alfred J.; Harter, Klaus (1 May 2010). "Fluorescence Intensity Decay Shape Analysis Microscopy (FIDSAM) for Quantitative and Sensitive Live-Cell Imaging: A Novel Technique for Fluorescence Microscopy of Endogenously Expressed Fusion-Proteins". Molecular Plant. 3 (3): 555–562. doi:10.1093/mp/ssp110.
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