Deoxyribonuclease II (EC 3.1.22.1, DNase II, pancreatic DNase II, deoxyribonucleate 3'-nucleotidohydrolase, pancreatic DNase II, acid deoxyribonuclease, acid DNase) is an endonuclease that hydrolyzes phosphodiester linkages of deoxyribonucleotide in native and denatured DNA, yielding products with 3'-phosphates and 5'-hydroxyl ends, which occurs as a result of single-strand cleaving mechanism.[1] As the name implies, it functions optimally at acid pH because it is commonly found in low pH environment of lysosomes.
The action of DNase occurs in three phases. The initial phase introduces multiple nicks in the phosphodiester backbone. The second phase produces acid-soluble nucleotides. The third phase, which is the terminal phase, consists of hyperchromic shift resulting from reduction of oligonucleotides.[1]
There are several known DNases II, including:
DNase II alpha (usually known as DNase II), which is thought to be ubiquitously expressed in human tissue.[2] It has been shown that a mutation in this enzyme of mice leads to DNA degradation by apoptosis.[3]
DNase II beta (also called DLAD, or DNase II-Like Acid DNase), which is mainly expressed in the eye lens and salivary glands. One of its functions is to clear DNA from eye lens. Low levels have also been detected in the lung, prostate and lymph nodes.[4] Deficiency of this enzyme in mice lead to the development of cataracts.
^ abBernardi G (1971). "Chapter 11: Spleen Acid Deoxyribonuclease". In Boyer PD (ed.). The Enzymes. Vol. 4 (3rd ed.). New York: Elsevier Science. pp. 271–287. doi:10.1016/S1874-6047(08)60371-6. ISBN 978-0-12-122704-3.
^Yasuda T, Takeshita H, Iida R, Tsutsumi S, Nakajima T, Hosomi O, Nakashima Y, Mori S, Kishi K (July 1998). "Structure and organization of the human deoxyribonuclease II (DNase II) gene". Annals of Human Genetics. 62 (Pt 4): 299–305. doi:10.1046/j.1469-1809.1998.6240299.x. PMID 9924608. S2CID 27515872.
^Universal protein resource accession number Q8WZ79 for "DNASE2B – Deoxyribonuclease-2-beta precursor – Homo sapiens (Human) – DNASE2B gene & protein" at UniProt.
and 22 Related for: Deoxyribonuclease II information
DeoxyribonucleaseII (EC 3.1.22.1, DNase II, pancreatic DNase II, deoxyribonucleate 3'-nucleotidohydrolase, pancreatic DNase II, acid deoxyribonuclease...
types of DNase found in animals are known as deoxyribonuclease I (DNase I) and deoxyribonucleaseII (DNase II). These two families have subcategories within...
Deoxyribonuclease 2 beta is a protein that in humans is encoded by the DNASE2B gene. The protein encoded by this gene shares considerable sequence similarity...
Kelly and Kent Wilcox isolated and characterized the first type II restriction enzyme, HindII, from the bacterium Haemophilus influenzae. Restriction enzymes...
II, endonuclease II, DNA-adenine-transferase) is catalyzes the degradation nucleotides in DsDNA by attacking the 5'-terminal end. Deoxyribonuclease IV...
DHX34: encoding protein Dexh-box helicase 34 DNASE2: encoding protein DeoxyribonucleaseII, lysosomal DPF1: encoding protein D4, zinc and double PHD fingers...
3' overhangs or blunt ends with no overhangs. EcoRI is an example of type II restriction enzymes which now has more the 300 enzymes with more than 200...
505. Shpak, M.; et al. (May 2008). "The phylogeny and evolution of deoxyribonucleaseII: an enzyme essential for lysosomal DNA degradation". Mol. Phylogenet...
Bibcode:1995Sci...269..656N. doi:10.1126/science.7624794. PMID 7624794. Deoxyribonuclease+BamHI at the U.S. National Library of Medicine Medical Subject Headings...
binding site on a particular DNA molecule. The method uses an enzyme, deoxyribonuclease (DNase, for short), to cut the radioactively end-labeled DNA, followed...
Stanford Moore on Nobelprize.org including the Nobel Lecture, December 11, 1972 The Chemical Structures of Pancreatic Ribonuclease and Deoxyribonuclease...
56-bp variable number of tandem repeat polymorphisms within the human deoxyribonuclease I gene". Legal Medicine. 17 (4): 283–6. doi:10.1016/j.legalmed.2015...
HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the...
protect the organism against invading foreign DNA. These site-specific deoxyribonucleases catalyse the endonucleolytic cleavage of DNA to give specific double-stranded...
spreading factor) breaks down hyaluronic acid and helps in spreading it. Deoxyribonuclease, which breaks down the DNA, protects S. aureus from neutrophil extracellular...
S2CID 205499369. Lacks S, Greenberg B, Neuberger M (June 1974). "Role of a deoxyribonuclease in the genetic transformation of Diplococcus pneumoniae". Proceedings...
dornase alfa and hypertonic saline. Dornase is a recombinant human deoxyribonuclease, which breaks down DNA in the sputum, thus decreasing its viscosity...
within a polynucleotide chain (namely DNA or RNA). Some, such as deoxyribonuclease I, cut DNA relatively nonspecifically (without regard to sequence)...
blood plasma fractionation was developed by Edwin J. Cohn during World War II. it's known as the Cohn process (or Cohn method). This process is also known...
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