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This article covers protein engineering[1] of cytochrome (CYP) P450 enzymes. P450s are involved in a range of biochemical catabolic and anabolic process.[2] Natural P450s can perform several different types of chemical reactions including hydroxylations, N,O,S-dealkylations, epoxidations, sulfoxidations, aryl-aryl couplings, ring contractions and expansions, oxidative cyclizations, alcohol/aldehyde oxidations, desaturations, nitrogen oxidations, decarboxylations, nitrations, as well as oxidative and reductive dehalogenations.[2][3] Engineering efforts often strive for 1) improved stability 2) improved activity 3) improved substrate scope 4) enabled ability to catalyze unnatural reactions.[4][5]
P450 engineering is an emerging field in the areas of chemical biology and synthetic organic chemistry (chemoenzymatic).
This article covers protein engineering of cytochrome (CYP) P450 enzymes. P450s are involved in a range of biochemical catabolic and anabolic process....
CytochromesP450 (P450s or CYPs) are a superfamily of enzymes containing heme as a cofactor that mostly, but not exclusively, function as monooxygenases...
CytochromeP450, family 76, also known as CYP76, is a cytochromeP450 family in land plants, related to the biosynthesis of many plant monoterpenes and...
CytochromeP450, family 105, also known as CYP105, is a cytochromeP450 monooxygenase family in bacteria, predominantly found in the phylum Actinomycetota...
massive overdoses (e.g., 20 g). Due to its non-selective inhibition of cytochromeP450 enzymes, cimetidine has numerous drug interactions. Examples of specific...
drug interactions is the enzyme system comprising the cytochromeP450 oxidases. CytochromeP450 is a very large family of haemoproteins (hemoproteins)...
synthesize ethynyl derivatives. 1-methylcyclohexene can be oxidized with a CytochromeP450 catalyst. The ratio of hydroxylation products to epoxidation products...
P450. Two types of P450 systems are found in eukaryotes. The P450 systems that are located in the endoplasmic reticulum are dependent on a cytochrome...
Phenobarbital is a cytochromeP450 hepatic enzyme inducer. It binds transcription factor receptors that activate cytochromeP450 transcription, thereby...
of enzymes involved in xenobiotic metabolism is hepatic microsomal cytochromeP450. These enzymes that metabolize xenobiotics are very important for the...
grapefruit and some other citrus fruits, through the inhibition of cytochromeP450-mediated metabolism of prescription drugs including anti-hypertensives...
1990). "Mechanism of corticotropin and cAMP induction of mitochondrial cytochromeP450 system enzymes in adrenal cortex cells". The Journal of Biological...
significantly reduces benzo[a]pyrene-DNA adduct formation and alters cytochromeP450 1A1 and 1B1 expression and EROD activity in normal human mammary epithelial...
bioinformatics and functional genomics. Her research focuses on studying cytochromeP450 enzymes and the effects of environmental contaminants on marine and...
which no previous specific enzyme existed, such as when she evolved cytochromeP450 to carry out cyclopropanation and carbene and nitrene transfer reactions...
ascorbate as cofactors and FNS II, a membrane bound, NADPH dependent cytochromep450 monooxygenase. The naturally occurring glycosides formed by the combination...
enzymes are used to yield GA from GGDP: terpene syntheses (TPSs), cytochromeP450 monooxygenases (P450s), and 2-oxoglutarate–dependent dioxygenases (2ODDs)...
overdose. Enzalutamide is a moderate to strong inducer of multiple cytochromeP450 enzymes including CYP3A4, CYP2C9, and CYP2C19 and hence has a high...
alkenes. Arene oxides are intermediates in the oxidation of arenes by cytochromeP450. For prochiral arenes (naphthalene, toluene, benzoates, benzopyrene)...
Gastrointestinal perforation Mucositis List of monoclonal antibodies Gelboin HV. "CytochromeP450 Mediated Drug and Carcinogen Metabolism using Monoclonal Antibodies"...
plays the most important role, followed by CYP2B6. In addition to the cytochromeP450 system, sertraline can be oxidatively deaminated in vitro by monoamine...
risk of cardiac valvulopathy. Ropinirole is metabolized primarily by cytochromeP450 CYP1A2 to form two metabolites; SK&F-104557 and SK&F-89124, both of...
apoptosis in the cells. Myristicin has also been shown to inhibit cytochrome P450 enzymes, which are responsible for metabolizing a variety of substrates including...
diterpene, taxol. In the third step, the lactone ring is formed by a cytochromeP450 enzyme using NADP+. With the butyloxy group on the C(8), the formation...
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