carbamoyl-phosphate synthetase 1, aspartate transcarbamylase, and dihydroorotase
Identifiers
Symbol
CAD
NCBI gene
790
HGNC
1424
OMIM
114010
RefSeq
NM_004341
UniProt
P27708
Other data
Locus
Chr. 2 p21
Search for
Structures
Swiss-model
Domains
InterPro
Carbamoyl phosphate synthetase (glutamine-hydrolysing) (EC 6.3.5.5) is an enzyme that catalyzes the reactions that produce carbamoyl phosphate in the cytosol (as opposed to type I, which functions in the mitochondria). Its systemic name is hydrogen-carbonate:L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating).[1][2][3][4][5][6][7][8]
In pyrimidine biosynthesis, it serves as the rate-limiting enzyme and catalyzes the following reaction:
It is activated by ATP and PRPP[9] and it is inhibited by UTP (Uridine triphosphate)[10]
Neither CPSI nor CPSII require biotin as a coenzyme, as seen with most carboxylation reactions.
It is one of the three enzyme functions coded by the CAD gene. It is classified under EC 6.3.5.5.
^Anderson PM, Meister A (December 1965). "Evidence for an activated form of carbon dioxide in the reaction catalyzed by Escherichia coli carbamyl phosphate synthetase". Biochemistry. 4 (12): 2803–9. doi:10.1021/bi00888a034. PMID 5326356.
^Kalman SM, Duffield PH, Brzozowski T (April 1966). "Purification and properties of a bacterial carbamyl phosphate synthetase". The Journal of Biological Chemistry. 241 (8): 1871–7. doi:10.1016/S0021-9258(18)96716-5. PMID 5329589.
^Yip MC, Knox WE (May 1970). "Glutamine-dependent carbamyl phosphate synthetase. Properties and distribution in normal and neoplastic rat tissues". The Journal of Biological Chemistry. 245 (9): 2199–204. doi:10.1016/S0021-9258(18)63139-4. PMID 5442268.
^Stapleton MA, Javid-Majd F, Harmon MF, Hanks BA, Grahmann JL, Mullins LS, Raushel FM (November 1996). "Role of conserved residues within the carboxy phosphate domain of carbamoyl phosphate synthetase". Biochemistry. 35 (45): 14352–61. doi:10.1021/bi961183y. PMID 8916922.
^Holden HM, Thoden JB, Raushel FM (December 1998). "Carbamoyl phosphate synthetase: a tunnel runs through it". Current Opinion in Structural Biology. 8 (6): 679–85. doi:10.1016/s0959-440x(98)80086-9. PMID 9914247.
^Raushel FM, Thoden JB, Reinhart GD, Holden HM (October 1998). "Carbamoyl phosphate synthetase: a crooked path from substrates to products". Current Opinion in Chemical Biology. 2 (5): 624–32. doi:10.1016/s1367-5931(98)80094-x. PMID 9818189.
^Raushel FM, Thoden JB, Holden HM (June 1999). "The amidotransferase family of enzymes: molecular machines for the production and delivery of ammonia". Biochemistry. 38 (25): 7891–9. doi:10.1021/bi990871p. PMID 10387030.
^Thoden JB, Huang X, Raushel FM, Holden HM (October 2002). "Carbamoyl-phosphate synthetase. Creation of an escape route for ammonia". The Journal of Biological Chemistry. 277 (42): 39722–7. doi:10.1074/jbc.M206915200. PMID 12130656.
^Inkling. "Unsupported Browser". Inkling. Retrieved 25 April 2018.
^Engelking LR. Pyrimidine biosynthesis. Textbook of Veterinary Physiological Chemistry. 2015;:83–7.
https://doi.org/10.1016/B978-0-12-391909-0.50014-1 Retrieved 1 April 2023
and 23 Related for: Carbamoyl phosphate synthase II information
Carbamoylphosphate synthetase (glutamine-hydrolysing) (EC 6.3.5.5) is an enzyme that catalyzes the reactions that produce carbamoylphosphate in the cytosol...
Carbamoylphosphate synthetase catalyzes the ATP-dependent synthesis of carbamoylphosphate from glutamine (EC 6.3.5.5) or ammonia (EC 6.3.4.16) and bicarbonate...
of chili peppers CarbamoylphosphatesynthaseII, an enzyme that catalyzes a reaction yielding carbamoylphosphateCarbamoylphosphate synthetase, an enzyme...
CPS II may refer to any of the following. CP System II, an arcade system board developed by Capcom CarbamoylphosphatesynthaseII, a catalyzing enzyme...
is converted into carbamoylphosphate through the investment of two ATP molecules. This step is facilitated by carbamoylphosphate synthetase I. The second...
a transfer is written as carbamoylphosphate + L-aspartate → {\displaystyle \rightarrow } L-carbamoyl aspartate + phosphate. Enzymes that transfer aldehyde...
CPS2 may refer to: CP System II, the Capcom Play System 2 CarbamoylPhosphateSynthaseII, the rate-limiting enzyme of pyrimidine biosynthesis Keene/Elmhirst's...
combination of 3-amino-5-hydroxybenzoic acid (AHBA), D-glucosamine, and carbamoylphosphate, to form the mitosane core, followed by specific tailoring steps...
of carbamoylphosphate from glutamine and CO2. Next, aspartate carbamoyltransferase catalyzes a condensation reaction between aspartate and carbamoyl phosphate...
CAD protein (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) is a trifunctional multi-domain enzyme involved in the...
synthase deficiency Online Mendelian Inheritance in Man (OMIM): 237300 - hyperammonemia due to carbamoylphosphate synthetase I deficiency (carbamoyl...
process are all coded by the same gene in CAD which consists of carbamoylphosphate synthetase II, aspartate carbamoyltransferase and dihydroorotase. Dihydroorotate...
citrullinemia type I needs to be distinguished from the others: carbamoylphosphate synthetase deficiency, argininosuccinic acid lyase deficiency, ornithine...
} ADP + carbamoylphosphate The 3 substrates of this enzyme are ATP, NH3, and CO2, whereas its two products are ADP and carbamoylphosphate. This enzyme...
negligible, as is the case with N-acetylglutamate's activity on carbamoylphosphate synthetase I, for example. A non-regulatory allosteric site is any...
increases, N-Acetylglutamate synthase is up-regulated, producing more N-acetyl-L-glutamate, which up-regulates carbamoylphosphate synthetase and allows it...
have been determined, to interpret test results. Inosine monophosphate synthase Nicotinamide riboside Zarembo, Alan (1 August 2008). "'Exercise pill' could...
and phosphates. Uracil serves as allosteric regulator and coenzyme for reactions in animals and in plants. UMP controls the activity of carbamoyl phosphate...
IUPAC name 4-[(5,5,8,8-Tetramethyl-5,6,7,8-tetrahydronaphthalen-2-yl)carbamoyl]benzoic acid Identifiers CAS Number 94497-51-5 3D model (JSmol) Interactive...
ribose 5-phosphate, a nucleotide precursor. Pyrimidines are shown to be synthesized from the reaction between aspartate and carbamoylphosphate at 60 °C...
Entinostat Names Preferred IUPAC name (Pyridin-3-yl)methyl ({4-[(2-aminophenyl)carbamoyl]phenyl}methyl)carbamate Other names SNDX-275; MS-275 Identifiers CAS Number...
inhibitor and an immunomodulator. As of 2007, it was undergoing multicenter phase II clinical trials. In 2016, early results in a small phase I trial for multiple...
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