Molecular biology method used to detect DNA fragmentation
Mouse liver showing an apoptotic cell stained with TUNEL
Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3′- hydroxyl termini in the double-strand DNA breaks generated during apoptosis.[1][2]
^Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z (1993). "Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. Analogy to apoptosis of somatic cells". Exp Cell Res. 207 (1): 202–205. doi:10.1006/excr.1993.1182. PMID 8391465.
^Lozano GM, Bejarano I, Espino J, González D, Ortiz A, García JF, Rodríguez AB, Pariente JA (2009). "Density gradient capacitation is the most suitable method to improve fertilization and to reduce DNA fragmentation positive spermatozoa of infertile men". Anatolian Journal of Obstetrics & Gynecology. 3 (1): 1–7.
Look up tunel in Wiktionary, the free dictionary. Tunel or Tünel may refer to: TUNELassay (Terminal deoxynucleotidyl transferase mediated dUTP Nick End...
identification of apoptotic cells either via the DNA laddering assay, the TUNELassay, or the by detection of cells with fractional DNA content ("sub...
homodimer assay instead of using a TUNELassay to measure cell death is that ethidium homodimer stains all of the dead or dying cells, while TUNEL only stains...
accumulates in a cell. It can be measured by e.g. the Comet assay or by the TUNELassay. Its main units of measurement is the DNA Fragmentation Index...
fragmentation revealed by the presence of double-strand breaks detected by the TUNELassay. Other techniques performed in order to measure the DNA fragmentation...
SCSA outcompetes other convention sperm DNA fragmentation (sDF) assays such as TUNEL and COMET in terms of efficiency, objectivity, and repeatability...
presence of double-strand breaks that can be detected by the TUNELassay. In this assay, the sperm's DNA will be denaturated and renatured. If DNA fragmentation...
upregulated, whereas the levels of Bcl-2, Bcl-w, and Mcl-1 were downregulated. TUNELassay, DNA laddering, and micronuclei induction show that long-term exposure...
add nucleotides labeled with radioactive isotopes, for example in the TUNELassay (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) for the...
investigated. Apoptotic DNA fragmentation Caspase-activated DNase Nicoletti assayTUNELassay Kressel, M.; Groscurth, P. (1994-12-01). "Distinction of apoptotic...
has been used as an assay to examine the pathology of cerebral organoids derived from individual patients. Additionally, TUNELassays have been used in...
to cell division. Darzynkiewicz is also recognized for developing the TUNELassay (terminal deoxynucleotidyl transferase dUTP nick end labeling), which...
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