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T7 phage information


Bacteriophage T7 Structural Model at Atomic Resolution[1]
Escherichia virus T7
Virus classification Edit this classification
(unranked): Virus
Realm: Duplodnaviria
Kingdom: Heunggongvirae
Phylum: Uroviricota
Class: Caudoviricetes
Order: Caudovirales
Family: Autographiviridae
Genus: Teseptimavirus
Species:
Escherichia virus T7

Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects bacteria. It infects most strains of Escherichia coli and relies on these hosts to propagate. Bacteriophage T7 has a lytic life cycle, meaning that it destroys the cell it infects. It also possesses several properties that make it an ideal phage for experimentation: its purification and concentration have produced consistent values in chemical analyses;[2] it can be rendered noninfectious by exposure to UV light;[3] and it can be used in phage display to clone RNA binding proteins.[3]

  1. ^ Dr. Victor Padilla-Sanchez, PhD (2021-07-10), Bacteriophage T7 Structural Model at Atomic Resolution., doi:10.5281/zenodo.5133295, retrieved 2021-07-24
  2. ^ Studier, F. William (1969-11-01). "The genetics and physiology of bacteriophage T7". Virology. 39 (3): 562–574. doi:10.1016/0042-6822(69)90104-4. ISSN 0042-6822. PMID 4902069.
  3. ^ a b Teesalu, Tambet; Sugahara, Kazuki N.; Ruoslahti, Erkki (1 January 2012). "Mapping of Vascular ZIP Codes by Phage Display". In Wittrup, K. Dane; Verdine, Gregory L. (eds.). Methods in Enzymology. Academic Press. pp. 35–56. doi:10.1016/B978-0-12-396962-0.00002-1. PMID 22230564.

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T7 phage

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Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects bacteria. It infects most strains of Escherichia coli and relies on these...

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T7 RNA polymerase

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initiation of the transcription. The consensus in T7 and related phages is: 5' * 3' T7   TAATACGACTCACTATAGGGAGA T3 AATTAACCCTCACTAAAGGGAGA K11 AATTAGGGCACACTATAGGGAGA...

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Phage display

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bacteriophages used in phage display are M13 and fd filamentous phage, though T4, T7, and λ phage have also been used. Phage display was first described...

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T7

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T7 or T-7 may refer to: Thoracic vertebra 7 Thoracic spinal nerve 7 T7 phage, a virus used in the study of biological systems T7 DNA Helicase, a hexameric...

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T7 expression system

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possesses a gene that is expressed to produce T7 RNA polymerase. (This polymerase originates from the T7 phage, a bacteriophage virus which infects E. coli...

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Bacteriophage

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186 phage λ phage Φ6 phage Φ29 phage ΦX174 Bacteriophage φCb5 G4 phage M13 phage MS2 phage (23–28 nm in size) N4 phage P1 phage P2 phage P4 phage R17...

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T7 DNA polymerase

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Nucleotidyl transfer by DNA polymerase. T7 DNA polymerase catalyzes the phosphoryl transfer during DNA replication of the T7 phage. As shown in Figure 2, the 3’...

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Escherichia virus T3

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T7 phage in structure though the two viruses may differ in capsid maturation. FRASER, D; WILLIAMS, RC (Feb 1953). "Details of frozen-dried T3 and T7 bacteriophages...

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T7 DNA helicase

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T7 DNA helicase (gp4) is a hexameric motor protein encoded by T7 phages that uses energy from dTTP hydrolysis to process unidirectionally along single...

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Operon

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bacteriophages. For example, T7 phages have two operons. The first operon codes for various products, including a special T7 RNA polymerase which can bind...

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Teseptimavirus

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Teseptimavirus (synonyms T7 phage group, T7-like phages, T7-like viruses, T7likevirus) is a genus of viruses in the order Caudovirales, in the family Autographiviridae...

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Phagemid

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Filamentous phages retard bacterial growth but, contrasting with the lambda phage and the T7 phage, are not generally lytic. Helper phages are usually...

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T7 holin family

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The T7 Holin family (TC# 1.E.6) is a member of the Holin Superfamily II. Members of this family are predominantly found in Caudovirales and Pseudomonadota...

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Primase

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DnaG primases can have extra functions, if given the right domains. The T7 phage gp4 is a DnaG primase-helicase fusion, and performs both functions in replication...

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Phage ecology

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Bacteriophages (phages), potentially the most numerous "organisms" on Earth, are the viruses of bacteria (more generally, of prokaryotes). Phage ecology is...

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MRNA vaccine

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promoter and sequence which corresponds to the mRNA construct. By combining T7 phage RNA polymerase and the plasmid DNA, the mRNA can be transcribed in the...

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List of microorganisms tested in outer space

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event and planetary ejection Atmospheric reentry Simulated conditions T7 phage Canine hepatitis Influenza PR8 Tobacco mosaic virus Vaccinia virus Yeast...

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Salmonella virus P22

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virion include bacteriophages λ and Ε34. Many Podoviridae, for example phages T7 and Φ29, share few DNA similarities with P22, even though their virion...

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GP5

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GP5 could refer to several things: GP5 (gene) T7 phage, or Gp5 GP5 chip, computer chip GP-5 gas mask, Soviet civilian gas mask This disambiguation page...

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Lysin

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when resistance is forced by mutagenesis experiments. Double-stranded DNA phage lysins tend to lie within the 25 to 40 kDa range in terms of size. A notable...

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Pseudomonas

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"Genomic Analysis of Pseudomonas aeruginosa Phages LKD16 and LKA1: Establishment of the φKMV Subgroup within the T7 Supergroup". Journal of Bacteriology. 188...

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Drew Endy

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from Dartmouth College in 1997 for his work on genetic engineering using T7 phage. Endy was a junior fellow for three years and later an Assistant Professor...

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Autographiviridae

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Enterobacteriaceae phages SP6 and K1-5 were the first to be considered as an estranged subgroup of the "T7 supergroup". Pseudomonas phage phiKMV also shared...

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Bacteriophage experimental evolution

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environment and mutation severity as revealed by in silico mutagenesis of phage T7. Genetics. 160:1273-1281. Schuppli, D., J. Georgijevic, and H. Weber. 2000...

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EXPOSE

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(Bacillus subtilis). PUR (ROSE-8), study of space environment effect on T7 phage, its DNA and of polycristalline uracil. IMBP (Institute of Biomedical Problems)...

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BP holin family

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small family that includes members derived from a number of Burkholderia phage as well as a Poloromonas species. As of April 3, 2016, this family belongs...

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Cloning vector

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and cloning vectors in E. coli include plasmids, bacteriophages (such as phage λ), cosmids, and bacterial artificial chromosomes (BACs). Some DNA, however...

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