T7 RNA polymerase information

T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5'→ 3' direction. T7 polymerase is...

In molecular biology, RNA polymerase (abbreviated RNAP or RNApol), or more specifically DNA-directed/dependent RNA polymerase (DdRP), is an enzyme that...

DNA-directed RNA polymerase (DdRP, RNAP) Multi-subunit (msDdRP): RNA polymerase I, RNA polymerase II, RNA polymerase III Single-subunit (ssDdRP): T7 RNA polymerase...

the T7 RNA polymerase and use it, along with the powerful T7 promoter, to transcribe copious amounts of almost any gene. The development of the T7 expression...

in the T3 and T7 RNA polymerases in bacteriophages and in E. coli. Abortive initiation occurs prior to promoter clearance. RNA polymerase binds to promoter...

DNA Helicase, a hexameric motor protein T7 RNA polymerase, an RNA polymerase that catalyzes the formation of RNA Île-de-France tramway Line 7 Olympic Park...

The first operon codes for various products, including a special T7 RNA polymerase which can bind to and transcribe the second operon. The second operon...

[citation needed] The T7 promoter sequence is used extensively in molecular biology due to its extremely high affinity for T7 RNA polymerase and thus high level...

cells, RNA polymerase III (also called Pol III) is a protein that transcribes DNA to synthesize 5S ribosomal RNA, tRNA, and other small RNAs. The genes...

basal level of the T7-RNA-polymerase is observable even without induction. T7 lysozyme acts as an inhibitor of the T7-RNA-polymerase. Newly invented strains...

T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands...

circumstances. Termination efficiency of T7 RNA polymerase is around 74%, which creates issues when T7 RNA polymerase is used to produce recombinant proteins...

virally encoded RNA-dependent RNA polymerase. For some virus families, VPg also has a role in translation initiation by acting like a 5' mRNA cap. VPg was...

contrast, RNA polymerases synthesize RNA from ribonucleotides from either RNA or DNA.[citation needed] When synthesizing new DNA, DNA polymerase can add...

for the recombinant gene is usually transcribed from DNA by a viral T7 RNA polymerase, which is not affected by rifampicin.[citation needed] In 1957, a...

for the transition from initiation to elongation transcription in T7 RNA polymerase". www.rcsb.org. Retrieved 27 March 2023. Pidoux AL, Allshire RC (March...

lacUV5 operon split by the DE3 prophage (and as a result driving the T7 RNA polymerase instead). The two important mutations are underlined. lacUV5...

comparison to copying of the same template by proteins such as the T7 RNA polymerase. An RPR called t5(+1) adds triplet nucleotides at a time instead of...

DNA-directed RNA polymerases, it is more closely related to RNA polymerases of bacteriophage (including T7 RNA polymerase), mitochondrial polymerases of lower...

host cell's RNA polymerase before the viral genome completely exits the capsid. Class II genes are then transcribed by T7 RNA polymerase and the host...

triphosphates (dNTPs) The polymerases used in RCA are Phi29, Bst, and Vent exo-DNA polymerase for DNA amplification, and T7 RNA polymerase for RNA amplification....

1994). "Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase". Nucleic Acids Res. 22 (14): 2784–90. doi:10.1093/nar/22.14.2784...

sequence which corresponds to the mRNA construct. By combining T7 phage RNA polymerase and the plasmid DNA, the mRNA can be transcribed in the lab. Efficacy...

to be produced by an RNA-dependent RNA polymerase (RdRP). MicroRNAs (miRNAs) are genomically encoded non-coding RNAs that help regulate gene expression...

DNA polymerase, they pair using an edge-to-edge conformation. dNaM and dTPT3 can also template transcription of mRNAs and tRNAs by T7 RNA polymerase that...

in the replication of DNA and is a type of RNA polymerase. Primase catalyzes the synthesis of a short RNA (or DNA in some living organisms) segment called...