Saturation mutagenesis of a single position in a theoretical 10-residue protein. The wild type version of the protein is shown at the top, with M representing the first amino acid methionine, and * representing the termination of translation. All 19 mutants at position 5 are shown below.
Site saturation mutagenesis (SSM), or simply site saturation, is a random mutagenesis technique used in protein engineering, in which a single codon or set of codons is substituted with all possible amino acids at the position.[1] There are many variants of the site saturation technique, from paired site saturation (saturating two positions in every mutant in the library) to scanning site saturation (performing a site saturation at every site in the protein, resulting in a library of size [20 x (number of residues in the protein)] that contains every possible point mutant of the protein).
^Reetz, M. T.; Carballeira J. D. (2007). "Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes". Nature Protocols. 2 (4): 891–903. doi:10.1038/nprot.2007.72. PMID 17446890. S2CID 37361631.
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possible point mutant of the protein). Saturationmutagenesis is commonly achieved by site-directed mutagenesis PCR with a randomised codon in the primers...
Sequence saturationmutagenesis (SeSaM) is a chemo-enzymatic random mutagenesis method applied for the directed evolution of proteins and enzymes.[citation...
Oxygen saturation, a clinical measure of the amount of oxygen in a patient's blood Saturation pollination, a pollination technique Saturated mutagenesis, a...
code. In nature mutagenesis can lead to cancer and various heritable diseases, and it is also a driving force of evolution. Mutagenesis as a science was...
Recursive ensemble mutagenesis then utilizes information from previous combinatorial cassette mutagenesis. Codon cassette mutagenesis allows you to insert...
between eukaryotes, archaea, and eubacteria. Gene site saturationmutagenesis (GSSM) is mutagenesis technique of one or more codons in a gene to create a...
Golden Gate Cloning principle can also be applied to perform mutagenesis termed Golden Mutagenesis. The technology is easy to implement as a web tool is available...
Protein design Expanded genetic code Xenobiology Mutagenesis: Random mutagenesis Saturated mutagenesis Staggered extension process Selection and screening:...
involves the use of several mutagenesis processes to induce DNA mutations at random which may include: Chemical mutagenesis is an easy tool that is used...
in some lasers Sequence saturationmutagenesis (SeSaM), a molecular biology method for diversity creation by random mutagenesis Sesame (disambiguation)...
transglycosylation activity of Thermus maltogenic amylase by combinatorial saturationmutagenesis". Journal of Microbiology and Biotechnology. 18 (8): 1401–1407....
approximately 200-fold, and permits ongoing measurement of genome saturation as mutagenesis progresses. Not only qualitative phenotypes, but subtle quantitative...
of the segmented anterior-posterior body axis of the fly; their "saturationmutagenesis" technique resulted in the discovery of a group of genes involved...
mutations can be introduced either semi-rationally or randomly via site-saturationmutagenesis. The resulting P450 mutant (typically mutant library) is then screened...
Muranaka N, Hohsaka T (March 2008). "Four-base codon-mediated saturationmutagenesis in a cell-free translation system". Journal of Bioscience and Bioengineering...
ISSN 0003-2697. PMID 20646988. Wong, T. S. (13 February 2004). "Sequence saturationmutagenesis (SeSaM): a novel method for directed evolution". Nucleic Acids Research...
via metagenomics, in addition to evolution technologies gene site saturationmutagenesis (GSSM) and GeneReassembly, and was the first to combine these discovery...
8419. PMC 390927. PMID 3909145. Chen, W; Struhl, K (1988-04-01). "Saturationmutagenesis of a yeast his3 TATA element: genetic evidence for a specific TATA-binding...
there he did his graduate work with Burke Judd. His work focused on saturationmutagenesis as well as the developmental genetics of the Zeste- White region...
Kenneth J.; Last, Robert L. (2003-01-01). "Ethylmethanesulfonate SaturationMutagenesis in Arabidopsis to Determine Frequency of Herbicide Resistance"....
These models are generated by methods including retroviral insertion mutagenesis or graft transplantation of cancerous cells. Mutations provide the raw...
A genetic screen or mutagenesis screen is an experimental technique used to identify and select individuals who possess a phenotype of interest in a mutagenized...
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