Sequence saturation mutagenesis (SeSaM) is a chemo-enzymatic random mutagenesis method applied for the directed evolution of proteins and enzymes.[citation needed] It is one of the most common saturation mutagenesis techniques. In four PCR-based reaction steps, phosphorothioate nucleotides are inserted in the gene sequence, cleaved and the resulting fragments elongated by universal or degenerate nucleotides. These nucleotides are then replaced by standard nucleotides, allowing for a broad distribution of nucleic acid mutations spread over the gene sequence with a preference to transversions and with a unique focus on consecutive point mutations, both difficult to generate by other mutagenesis techniques. The technique was developed by Professor Ulrich Schwaneberg at Jacobs University Bremen and RWTH Aachen University.
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Sequencesaturationmutagenesis (SeSaM) is a chemo-enzymatic random mutagenesis method applied for the directed evolution of proteins and enzymes.[citation...
possible point mutant of the protein). Saturationmutagenesis is commonly achieved by site-directed mutagenesis PCR with a randomised codon in the primers...
code. In nature mutagenesis can lead to cancer and various heritable diseases, and it is also a driving force of evolution. Mutagenesis as a science was...
therapy. In directed evolution, random mutagenesis, e.g. by error-prone PCR or sequencesaturationmutagenesis, is applied to a protein, and a selection...
used in some lasers Sequencesaturationmutagenesis (SeSaM), a molecular biology method for diversity creation by random mutagenesis Sesame (disambiguation)...
between eukaryotes, archaea, and eubacteria. Gene site saturationmutagenesis (GSSM) is mutagenesis technique of one or more codons in a gene to create a...
damage to the DNA sequence of an organism. Mutations can also be generated by insertional mutagenesis. Most often, insertional mutagenesis involves the use...
Golden Gate Cloning principle can also be applied to perform mutagenesis termed Golden Mutagenesis. The technology is easy to implement as a web tool is available...
Protein design Expanded genetic code Xenobiology Mutagenesis: Random mutagenesis Saturated mutagenesis Staggered extension process Selection and screening:...
ISSN 0003-2697. PMID 20646988. Wong, T. S. (13 February 2004). "Sequencesaturationmutagenesis (SeSaM): a novel method for directed evolution". Nucleic Acids...
Alternatively, ENU mutagenesis is a common random mutagenesis gene knockout strategy in the mouse that can also be used in the rat. ENU mutagenesis involves using...
hypermutagenesis in immunoglobulin genes. II. Influence of neighbouring base sequences on mutagenesis". Biochim. Biophys. Acta. 1171 (1): 11–8. doi:10.1016/0167-4781(92)90134-l...
A genetic screen or mutagenesis screen is an experimental technique used to identify and select individuals who possess a phenotype of interest in a mutagenized...
vasodilatation, then a bolus of contrast medium is administered while acquiring saturation recovery images of the heart with a high temporal resolution readout....
approximately 200-fold, and permits ongoing measurement of genome saturation as mutagenesis progresses. Not only qualitative phenotypes, but subtle quantitative...
unlike uncatalysed chemical reactions, enzyme-catalysed reactions display saturation kinetics. For a given enzyme concentration and for relatively low substrate...
obtained. These studies, in addition to investigations using site-directed mutagenesis of specific amino acids, have identified several residues that are crucial...
after. The primary sequence of this protein in Arabidopsis is listed below. Residues involved in catalytic activity are bolded. Mutagenesis of Asp428, which...
of the segmented anterior-posterior body axis of the fly; their "saturationmutagenesis" technique resulted in the discovery of a group of genes involved...
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