Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Chemical compound
Resazurin (7-Hydroxy-3H-phenoxazin-3-one 10-oxide) is a phenoxazine dye that is weakly fluorescent, nontoxic, cell-permeable, and redox‐sensitive.[2][3] Resazurin has a blue to purple color above pH 6.5 and an orange color below pH 3.8.[4] It is used in microbiological, cellular, and enzymatic assays because it can be irreversibly reduced to the pink-colored and highly fluorescent resorufin (7-Hydroxy-3H-phenoxazin-3-one). At circum-neutral pH, resorufin can be detected by visual observation of its pink color or by fluorimetry, with an excitation maximum at 530-570 nm and an emission maximum at 580-590 nm.[5]
When a solution containing resorufin is submitted to reducing conditions (Eh < -110 mV), almost all resorufin is reversibly reduced to the translucid non-fluorescent dihydroresorufin (also known as hydroresorufin) and the solution becomes translucid (the redox potential of the resorufin/dihydroresorufin pair is -51 mV vs. standard hydrogen electrode at pH 7.0). When the Eh of this same solution is increased, dihydroresorufin is oxidized back to resorufin, and this reversible reaction can be used to monitor if the redox potential of a culture medium remains at a sufficiently low level for anaerobic organisms.
Resazurin(pH indicator)
below pH 3.8
above pH 6.5
3.8
⇌
6.5
Resazurin solution has one of the highest values known of Kreft's dichromaticity index.[6] This means that it has a large change in perceived color hue when the thickness or concentration of observed sample increases or decreases.
Usually, resazurin is available commercially as the sodium salt.
^"Resazurin | C12H7NO4 | ChemSpider".
^Bueno, C.; Villegas, M. L.; Bertolotti, S. G.; Previtali, C. M.; Neumann, M. G.; Encinas, M. V. (2002). "The Excited-State Interaction of Resazurin and Resorufin with Aminesin Aqueous Solutions. Photophysics and Photochemical Reaction". Photochemistry and Photobiology. 76 (4): 385–90. doi:10.1562/0031-8655(2002)0760385TESIOR2.0.CO2. PMID 12405144. S2CID 222102027.
^Haggerty, Roy; Martí, Eugènia; Argerich, Alba; Schiller, Daniel von; Grimm, Nancy B. (2009). "Resazurin as a "smart" tracer for quantifying metabolically active transient storage in stream ecosystems". Journal of Geophysical Research: Biogeosciences. 114 (G3). Bibcode:2009JGRG..114.3014H. doi:10.1029/2008JG000942. hdl:10261/38263. ISSN 2156-2202.
^Chen, J. L., Steele, T. W., & Stuckey, D. C. (2015). Modeling and application of a rapid fluorescence-based assay for biotoxicity in anaerobic digestion. Environmental science & technology, 49(22), 13463-13471.http://pubs.acs.org/doi/10.1021/acs.est.5b03050
^Kreft, Samo; Kreft, Marko (2009). "Quantification of dichromatism: A characteristic of color in transparent materials". Journal of the Optical Society of America A. 26 (7): 1576–81. Bibcode:2009JOSAA..26.1576K. doi:10.1364/JOSAA.26.001576. PMID 19568292.
Resazurin (7-Hydroxy-3H-phenoxazin-3-one 10-oxide) is a phenoxazine dye that is weakly fluorescent, nontoxic, cell-permeable, and redox‐sensitive. Resazurin...
salt balance and leucocytes reduce resazurin dye faster. Mastitic samples give delayed coagulation but faster resazurin reduction compared to normal milk...
redox-based assay has also been developed using the fluorescent dye, resazurin. In addition to using dyes to indicate the redox potential of cells in...
promotes the distinction of cell viability in a specimen. Resazurin reduction: Resazurin Reduction Assays perform very closely to that of a tetrazolium...
counter, Electric cell-substrate impedance sensing Cell viability assays: resazurin method, ATP test, Ethidium homodimer assay (detect dead or dying cells)...
cytoplasmic enzymes (esterases). The MTT assays (colorimetric) and the resazurin assay (fluorimetric) dose the mitochondrial redox potential. All these...
is the starting material for the initiating explosive lead styphnate. Resazurin, C12H7NO4, obtained by the action of nitrous acid on resorcinol, forms...
of dichromatic substances are pumpkin seed oil, bromophenol blue, and resazurin. When the layer of pumpkin seed oil is less than 0.7 mm thick, the oil...
color will turn to red or pink depend on the amount of resazurin in the solution. More resazurin will result in more time needed for the solution to turn...
(Rustum et al., 2008). A surrogate to BOD5 has been developed using a resazurin derivative which reveals the extent of oxygen uptake by micro-organisms...
glove box, anaerobic jar, or with the use of Hungate tubes, syringes, and resazurin oxygen indicator. It is also recommended to grow strains in media with...
growth can also occur on media in a chamber without oxygen present. Sodium resazurin can be added to indicate redox potential. Cultures are to be incubated...
Mannan, A. (2019). Phytochemical profiling and evaluation of modified resazurin microtiter plate assay of the roots of Trillium govanianum. Natural Product...
samples were obtained had a pH adjusted to 6.0-6.5 and included 1mg of resazurin, 1mL of trace element and vitamin solutions, and 0.2 g of yeast extract...
oxygen had permeated the sample, researchers used the oxygen indicator resazurin. Growth was achieved by enrichment with Brock's Sulfolobus medium, which...
exposed to ultraviolet radiation. The use of a paii based on the dye resazurin forms the basis of an ISO standard test for photocatalytic activity. A...
semi-finished and ready-to-eat meat and fish products, made by using resazurin test. 1970 Express methods of Sanitary-bacteriological evaluation of foodstuffs-rapid...
medium with energy-depleted rumen fluid (at 40% v/v), sodium carbonate, resazurin, L-cysteine-HCl, lipoic acid, vitamins, minerals, and Amisoy (a partially...
hydrated MgSO4, MnSO4, a solution of KH2PO4, cysteine hydrochloride, and resazurin. 47 separate strains of anaerobic gram-negative bacteria were observed...
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