Recombineering (recombination-mediated genetic engineering)[1] is a genetic and molecular biology technique based on homologous recombination systems, as opposed to the older/more common method of using restriction enzymes and ligases to combine DNA sequences in a specified order. Recombineering is widely used for bacterial genetics, in the generation of target vectors for making a conditional mouse knockout, and for modifying DNA of any source often contained on a bacterial artificial chromosome (BAC), among other applications.
^Ellis H. M., Yu D., DiTizio T., Court D. L. (2001). "High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides". Proceedings of the National Academy of Sciences. 98 (12): 6742–6746. Bibcode:2001PNAS...98.6742E. doi:10.1073/pnas.121164898. PMC 34423. PMID 11381128.{{cite journal}}: CS1 maint: multiple names: authors list (link)
Recently, recombineering has been developed for high throughput DNA engineering applications termed 'recombineering pipelines'. Recombineering pipelines...
antigen, a gene family Multiplex Automated Genome Engineering, in genetic recombineering MAGE-OM and MAGE-TAB, standards by the FGED Society Eugène Mage (1837-1869)...
Land Register, a British database of land ownership Retron Library Recombineering, a genetic technique that uses retrons This disambiguation page lists...
Magnuson T (2013). "A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. Coli". PLOS ONE. 8 (2): e57075. Bibcode:2013PLoSO...857075J. doi:10...
February 2013). "A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli". PLOS ONE. 8 (2): e57075. Bibcode:2013PLoSO...857075J. doi:10...
called 'exo'), beta and gamma in the DNA engineering method called recombineering. The 48 kb DNA fragment of lambda phage is not essential for productive...
knockdown strain is extremely fast and requires only one-step oligo recombineering. The requirement of a protospacer adjacent motif (PAM) sequence limits...
Copeland, Neal G.; Jenkins, Nancy A.; Court, Donald L. (October 2001). "Recombineering: a powerful new tool for mouse functional genomics". Nature Reviews...
bacteriophage lambda are used in recombination. This mechanism, known as recombineering, utilizes the three proteins Exo, Beta, and Gam, which are created by...
Wang E. Full-length 3' Un-Translated Region reporter construction with recombineering. Analytical Biochemistry. 424: 162-167 (2012) PMID 22366478 "Eugenia...
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