Rapid Stain Identification Series (RSID) is designed for fast, easy and reliable detection of human fluids from a variety of samples encountered by forensic laboratories.
It has been developed in the United States for the Federal Bureau of Investigation.[citation needed]
This group of tests relies on antibody conjugation reactions to form colored complexes in the presence of specific biological fluids. Most tests are also designed so that only specific human fluids will end in a positive result. To perform a test, the sample in question is mixed with a buffer specific to the RSID test. The sample is then deposited onto the sample window where it is pulled through the test and control regions by a paper wick via passive diffusion. The RSID unit is impregnated with fluid-specific antibodies conjugated to colored complexes that will re-dissolve and diffuse at this time. If the fluid in question is present in the questioned sample, antibodies at the test line will react to the colored antibody complexes and form a colored line. If no fluid is present, or if the sample is not human, this line will not appear. A line will form at the control region regardless of if the sample contains body fluid or not, as it acts as an internal control.[1][2][3][4]
Positive and negative controls are also performed alongside each test to confirm its reliability and functionality. Positive controls are swabs taken from known bodily fluids while negative controls are buffer without any biological sample.[1][2][3][4]
^ ab"Rapid Stain Identification of Human Saliva (RSID -Saliva)." Independent Forensics, Nov. 2016, ifi-test.com/documents/short-incub-SALIVA-Universal-Buffer-11-2016.pdf
^ ab"Rapid Stain Identification of Human Blood (RSID -Blood)." Independent Forensics, April 2016, ifi-test.com/documents/BLOOD-Universal-Buffer--SpinEze062816-.pdf.
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