The optical properties of all liquid and solid materials change as a function of the wavelength of light used to measure them. This change as a function of wavelength is called the dispersion of the optical properties. The graph created by plotting the optical property of interest by the wavelength at which it is measured is called a dispersion curve.
The dispersion staining is an analytical technique used in light microscopy that takes advantage of the differences in the dispersion curve of the refractive index of an unknown material relative to a standard material with a known dispersion curve to identify or characterize that unknown material. These differences become manifest as a color when the two dispersion curves intersect for some visible wavelength. This is an optical staining technique and requires no stains or dyes to produce the color. Its primary use today is in the confirmation of the presence of asbestos in construction materials[1][2][3] but it has many other applications.[4][5][6][7][8][9]
^"Archived copy" (PDF). Archived from the original (PDF) on 2008-12-17. Retrieved 2008-07-19.{{cite web}}: CS1 maint: archived copy as title (link) US-EPA Bulk Asbestos Test Method 600/R-93/116 page 16
^"Archived copy" (PDF). Archived from the original (PDF) on 2008-10-07. Retrieved 2008-07-19.{{cite web}}: CS1 maint: archived copy as title (link) US-NIOSH Bulk Asbestos Test Method 9002, pages 4 and 5
^[1] US-OSHA Bulk Asbestos Test Method D-191, Section 4.6
^Kohlbeck, J.A. and W.T. Bolleter, “Microscopic determination of the degree of nitration of nitrocellulose with dispersion staining”, JOURNAL OF APPLIED POLYMER SCIENCE, vol 12, no.1, pp. 131-135, 1968
^Su, Shu-Chun, “Dispersion Staining - A versatile complement to Becke line method for refractive index determination”, GEOCHIMICA ET COSMOCHEMICA ACTA SUPPLEMENT, vol. 69, Issue 10, Supplement 1, Goldschmidt Conference Abstracts 2005., p.A727, 2005
^[2] US-FBI Method for Characterizing Trace Glass Evidence
^[3] Characterizing the chemical composition of the mineral olivine
^Crutcher, E. R., “Optical Microscopy: An Important Tool for Particulate Receptor Source Apportionment”, PROCEEDINGS OF THE AIR POLLUTION CONTROL ASSOCIATION, page 272, 1981. This paper includes dispersion staining as part of the body of methods used to identify the sources of air pollution in urban environments.
^Crutcher, E. R., “The Role of Light Microscopy in Aerospace Analytical Laboratories”, PROCEEDINGS OF THE NINTH SPACE SIMULATION SYMPOSIUM, 1977. This paper includes dispersion staining as part of the body of methods used to identify the sources of contamination in aerospace or micro-electronic clean rooms.
and 25 Related for: Dispersion staining information
color when the two dispersion curves intersect for some visible wavelength. This is an optical staining technique and requires no stains or dyes to produce...
Trichrome staining is a histological staining method that uses two or more acid dyes in conjunction with a polyacid. Staining differentiates tissues by...
quite variable. Dispersionstaining is an optical technique that results in a colored image of a colorless object. This is an optical staining technique and...
is stained, leaving the actual specimen untouched, and thus visible. This contrasts with positive staining, in which the actual specimen is stained. For...
high standards in stained glass painting and staining, to act as a locus for the exchange of information and ideas within the stained glass craft and to...
In chemistry, bromocresol green is used in Thin-layer chromatography staining solutions to visualize acidic compounds. In aqueous solution, bromocresol...
(1989). YashRoy R.C. (1990) Lamellar dispersion and phase separation of chloroplast membrane lipids by negative staining electron microscopy. Journal of Biosciences...
may present a cosmetic outer problem only (primary efflorescence causing staining), but can sometimes indicate internal structural weakness (migration/degradation...
mixed lipid polymorphism in aqueous dispersions can be easily identified and characterized by negative staining transmission electron microscopy too...
gel may be stained later after electrophoresis. Destaining of the gel is not necessary but may produce better images. Other methods of staining are available;...
both analog and digital photography. Chromatic aberration due to optical dispersion through a lens, leading to color fringes at high-contrast boundaries in...
technique of staining fluid oil paint and house paint into raw canvas. During 1951 he produced a series of semi-figurative black stain paintings, and...
hexagonal aqueous-lipid structures, in aqueous dispersions of membrane lipids. As water-soluble negative stain is excluded from the hydrophobic part (fatty...
with lipofuscin pigment. H&E stain. Prussian blue staining, highlighting the hemosiderin pigment as blue. Prussian blue stain Engineer's blue, Prussian blue...
corn are not considered host crops, and the last has been shown to slow dispersion of green stink bug to nearby host crops due to its tall field edges. Adults...
sending them to all the nations and giving them power over demons. The dispersion of the Apostles in the traditional ending of Mark is thought to be a 2nd-century...
"Sustainable and High Performance: New Biobased Acrylic Dispersion to Prevent Knot Staining for Wood Coatings". CoatingsTech: 40–47. Archived from the...
YashRoy R.C. (1990). "Lamellar dispersion and phase separation of chloroplast membrane lipids by negative staining electron microscopy" (PDF). Journal...
from ultraviolet light Heat stability Toxicity Tinting strength StainingDispersion (which can be measured with a Hegman gauge) Opacity or transparency...
water-containing substances wet PTFE, as fluorocarbons exhibit only small London dispersion forces due to the low electric polarizability of fluorine. PTFE has one...
chemicals, corrosives and staining agents. There are two ways to apply PVF, either as a preformed film (laminating) or from dispersion (coating). These coatings...
precipitation, chelation, or ion exchange. In addition, they help remove soil by dispersion. The earliest builders were sodium carbonate (washing soda) and sodium...
1.8. This heightened refractive index also correlates with increased dispersion, which measures the degree to which a medium separates light into its...
selector) or water in which case they are referred to as emulsions or dispersions and they are also provided in 100% solids bead form. One example is...
red, often marketed as "cranberry". The color is caused by the size and dispersion of gold particles. Ruby gold glass is usually made of lead glass with...
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