A UV radiation induced thymine-thymine cyclobutane dimer (right) is the type of DNA damage which is repaired by DNA photolyase. Note: The above diagram is incorrectly labelled as thymine as the structures lack 5-methyl groups.
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EC no.
4.1.99.3
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37290-70-3
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Photolyases (EC 4.1.99.3) are DNA repair enzymes that repair damage caused by exposure to ultraviolet light. These enzymes require visible light (from the violet/blue end of the spectrum) both for their own activation[1] and for the actual DNA repair.[2] The DNA repair mechanism involving photolyases is called photoreactivation. They mainly convert pyrimidine dimers into a normal pair of pyrimidine bases. Photo reactivation, the first DNA repair mechanism to be discovered, was described initially by Albert Kelner in 1949[3] and independently by Renato Dulbecco also in 1949.[4][5][6]
^Yamamoto J, Shimizu K, Kanda T, Hosokawa Y, Iwai S, Plaza P, Müller P (October 2017). "Loss of Fourth Electron-Transferring Tryptophan in Animal (6-4) Photolyase Impairs DNA Repair Activity in Bacterial Cells". Biochemistry. 56 (40): 5356–5364. doi:10.1021/acs.biochem.7b00366. PMID 28880077.
^Thiagarajan V, Byrdin M, Eker AP, Müller P, Brettel K (June 2011). "Kinetics of cyclobutane thymine dimer splitting by DNA photolyase directly monitored in the UV". Proceedings of the National Academy of Sciences of the United States of America. 108 (23): 9402–7. Bibcode:2011PNAS..108.9402T. doi:10.1073/pnas.1101026108. PMC 3111307. PMID 21606324.
^Kelner, A. 1949 Effect of visible light on the recovery of Streptomyces griseus conidia from ultra-violet irradiation injury. Proc. Natl. Acad. Sci. U. S., 35, 73-79
^Dulbecco R. Reactivation of ultra-violet-inactivated bacteriophage by visible light. Nature. 1949 Jun 18;163(4155):949. doi: 10.1038/163949b0. PMID 18229246
^Dulbecco R. Experiments on photoreactivation of bacteriophages inactivated with ultraviolet radiation. J Bacteriol. 1950 Mar;59(3):329-47. doi: 10.1128/jb.59.3.329-347.1950. PMID 15436402; PMCID: PMC385765
^Friedberg EC. A history of the DNA repair and mutagenesis field: I. The discovery of enzymatic photoreactivation. DNA Repair (Amst). 2015 Sep;33:35-42. doi: 10.1016/j.dnarep.2015.06.007. Epub 2015 Jun 24. PMID 26151545
Photolyases (EC 4.1.99.3) are DNA repair enzymes that repair damage caused by exposure to ultraviolet light. These enzymes require visible light (from...
domains with photolyase proteins. Isoform d nucleotide positions 6 through 491 show a conserved domain with deoxyribodipyrimidine photolyase, and positions...
their mechanistic studies of DNA repair. He has made contributions on photolyase and nucleotide excision repair in bacteria that have changed his field...
DNA photolyase may refer to: DNA photolyase (protein domain) Deoxyribodipyrimidine photo-lyase, an enzyme (6-4)DNA photolyase, an enzyme This set index...
ability to produce gadusol, a chemical which protects against the sun. The photolyase DNA repair mechanism, which relies on visible light, does not work in...
through photolyase reactivation or nucleotide excision repair, with the latter being prevalent in humans. Conversely, certain bacteria utilize photolyase, powered...
large increases in expression of two DNA repair genes (one gene product a photolyase and the other, a protein involved in nucleotide excision repair). Perennials...
"Computational Assignment of the Histidine Protonation State in (6-4) Photolyase Enzyme and Its Effect on the Protonation Step". ACS Catalysis. 6 (8):...
uvrA, uvrB and uvrC (that encode the UvrABC endonuclease) as well as the photolyase phr2 gene. The uvrA, uvrB and uvrC genes are upregulated upon UVC irradiation...
a DNA-repair-enzyme photolyase. As its prefix implies, photolyase is reliant on light in order to repair the strand. Photolyase works with its cofactor...
with the processing of uric acid Photolyase pseudogene, associated with repairing DNA damaged by UV radiation. Photolyase is no longer encoded for despite...
photolyase, whose activation is obligately dependent on energy absorbed from blue/UV light (300–500 nm wavelength) to promote catalysis. Photolyase,...
flavin as a chromophore. The cryptochromes have evolved from microbial DNA-photolyase, an enzyme that carries out light-dependent repair of UV damaged DNA....
contain the radioactive label. The method was used for amplification of photolyase in Escherichia coli. Sancar A, Hack AM, Rupp WD. J Bacteriol. 1979 Simple...
existed in a UV environment because of the prevalence of photolyase across the tree of life. Photolyase uses UV to drive photoreactivation, a mechanism that...
excision repair enzymes. In most organisms, they can also be repaired by photolyases, a light-dependent family of enzymes. Xeroderma pigmentosum is a genetic...
dimers (CPDs) induced by UV light can be repaired by expression of CPD photolyase. On May 12, 2022, NASA announced that specimens of Arabidopsis thaliana...
associated with the genes recA, lexA, uvrA, uvrB, and uvrC, in addition to a photolyase gene that is expressed when the environment offers excessive ultraviolet...
large increases in expression of two DNA repair genes (one gene product a photolyase and the other, a protein employed in nucleotide excision repair). Roots...
other environmental parameters. Salamanders and frogs that produce less photolyase, an enzyme that counteracts DNA damage from UVB, are more susceptible...
In the lesser species, they can repair the damage to the DNA using the photolyase enzyme, but higher species do not have this enzyme. In human cells, repair...
(FbiC) produces Coenzyme FO (also written F0), itself a cofactor of DNA photolyase (antenna). This is the head portion of the molecule. 2-phospho-L-lactate...
R.; Machius, M.; Deisenhofer, J. (6 August 2004). "Structure of the photolyase-like domain of cryptochrome 1 from Arabidopsis thaliana". Proceedings...
blue-light reception and regulation, de-etiolation, circadian rhythm, and photolyase. In the photoactivation process, blue light hits cryptochrome and accepts...
nanozymes were used to detect hydrogen sulfide in the brains of living rats. Photolyase-like CeO2 was reported. An editorial on nanozymes titled "Can Nanozymes...
internal light in photoreactivation, a DNA repair process carried out by photolyase. Experiments have shown that non-luminescent V. harveyi mutants are more...
through proteosome-dependent ubiquitin-mediated degradation. The CRY's photolyase homology domain is used for light detection and phototransduction, whereas...
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