Global Information Lookup Global Information

Optical transfection information


Optical transfection is a biomedical technique that entails introducing nucleic acids (i.e. genetic material such as DNA) into cells using light. All cells are surrounded by a plasma membrane, which prevents many substances from entering or exiting the cell. Lasers can be used to burn a tiny hole in this membrane, allowing substances to enter. This is tremendously useful to biologists who are studying disease, as a common experimental requirement is to put things (such as DNA) into cells.

Typically, a laser is focussed to a diffraction limited spot (~1 μm diameter) using a high numerical aperture microscope objective. The plasma membrane of a cell is then exposed to this highly focussed light for a small amount of time (typically tens of milliseconds to seconds), generating a transient pore on the membrane. The generation of a photopore[check spelling] allows exogenous plasmid DNA, RNA, organic fluorophores, or larger objects such as semiconductor quantum nanodots to enter the cell. In this technique, one cell at a time is treated, making it particularly useful for single cell analysis.

This technique was first demonstrated in 1984 by Tsukakoshi et al., who used a frequency tripled Nd:YAG to generate stable and transient transfection of normal rat kidney cells.[1] Since this time, the optical transfection of a host of mammalian cell types has been demonstrated using a variety of laser sources, including the 405 nm continuous wave (cw),[2] 488 nm cw,[3] or pulsed sources such as the 800 nm femtosecond pulsed Ti:Sapphire[4][5][6][7][8][9][10][11][12][13] or 1064 nm nanosecond pulsed Nd:YAG.[14][15]

  1. ^ Tsukakoshi, M.; Kurata, S.; Nomiya, Y.; Ikawa, Y.; Kasuya, T. (1984). "A novel method of DNA transfection by laser microbeam cell surgery". Applied Physics B: Photophysics and Laser Chemistry. 35 (3). Springer Science and Business Media LLC: 135–140. Bibcode:1984ApPhB..35..135T. doi:10.1007/bf00697702. ISSN 0721-7269. S2CID 123250337.
  2. ^ Paterson, L.; Agate, B.; Comrie, M.; Ferguson, R.; Lake, T. K.; et al. (2005). "Photoporation and cell transfection using a violet diode laser". Optics Express. 13 (2). The Optical Society: 595–600. Bibcode:2005OExpr..13..595P. doi:10.1364/opex.13.000595. ISSN 1094-4087. PMID 19488389.
  3. ^ Palumbo, Giuseppe; Caruso, Matilde; Crescenzi, Elvira; Tecce, Mario F.; Roberti, Giuseppe; Colasanti, Alberto (1996). "Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation". Journal of Photochemistry and Photobiology B: Biology. 36 (1). Elsevier BV: 41–46. doi:10.1016/s1011-1344(96)07335-6. ISSN 1011-1344. PMID 8988610.
  4. ^ Tsampoula, X.; Taguchi, K.; Čižmár, T.; Garces-Chavez, V; Ma, N.; et al. (2008-10-10). "Fibre based cellular transfection". Optics Express. 16 (21). The Optical Society: 17007–13. Bibcode:2008OExpr..1617007T. doi:10.1364/oe.16.017007. ISSN 1094-4087. PMID 18852810.
  5. ^ Uchugonova, Aisada; König, Karsten; Bueckle, Rainer; Isemann, Andreas; Tempea, Gabriel (2008-06-11). "Targeted transfection of stem cells with sub-20 femtosecond laser pulses". Optics Express. 16 (13). The Optical Society: 9357–64. Bibcode:2008OExpr..16.9357U. doi:10.1364/oe.16.009357. ISSN 1094-4087. PMID 18575499.
  6. ^ Brown, C. T. A.; Stevenson, D. J.; Tsampoula, X.; McDougall, C.; Lagatsky, A. A.; et al. (2008). "Enhanced operation of femtosecond lasers and applications in cell transfection". Journal of Biophotonics. 1 (3). Wiley: 183–199. doi:10.1002/jbio.200810011. ISSN 1864-063X. PMID 19412968. S2CID 10801756.
  7. ^ Baumgart, J.; Bintig, W.; Ngezahayo, A.; Willenbrock, S.; Murua Escobar, H.; Ertmer, W.; Lubatschowski, H.; Heisterkamp, A. (2008). "Quantified femtosecond laser based opto-perforation of living GFSHR-17 and MTH53 a cells". Optics Express. 16 (5). The Optical Society: 3021–31. Bibcode:2008OExpr..16.3021B. doi:10.1364/oe.16.003021. ISSN 1094-4087. PMID 18542388.
  8. ^ Lei, Ming; Xu, Hanpeng; Yang, Hao; Yao, Baoli (2008). "Femtosecond laser-assisted microinjection into living neurons". Journal of Neuroscience Methods. 174 (2). Elsevier BV: 215–218. doi:10.1016/j.jneumeth.2008.07.006. ISSN 0165-0270. PMID 18687359. S2CID 10242427.
  9. ^ Tsampoula, X.; Garcés-Chávez, V.; Comrie, M.; Stevenson, D. J.; Agate, B.; Brown, C. T. A.; Gunn-Moore, F.; Dholakia, K. (2007-07-30). "Femtosecond cellular transfection using a nondiffracting light beam". Applied Physics Letters. 91 (5). AIP Publishing: 053902–053903. Bibcode:2007ApPhL..91e3902T. doi:10.1063/1.2766835. ISSN 0003-6951.
  10. ^ Peng, Cheng; Palazzo, Robert E.; Wilke, Ingrid (2007-04-03). "Laser intensity dependence of femtosecond near-infrared optoinjection". Physical Review E. 75 (4). American Physical Society (APS): 041903. Bibcode:2007PhRvE..75d1903P. doi:10.1103/physreve.75.041903. ISSN 1539-3755. PMID 17500917.
  11. ^ Stevenson, D.; Agate, B.; Tsampoula, X.; Fischer, P.; Brown, C. T. A.; Sibbett, W.; Riches, A.; Gunn-Moore, F.; Dholakia, K. (2006). "Femtosecond optical transfection of cells:viability and efficiency". Optics Express. 14 (16). The Optical Society: 7125–33. Bibcode:2006OExpr..14.7125S. doi:10.1364/oe.14.007125. ISSN 1094-4087. PMID 19529083.
  12. ^ Barrett, Lindy E; Sul, Jai-Yoon; Takano, Hajime; Van Bockstaele, Elisabeth J; Haydon, Philip G; Eberwine, James H (2006-05-23). "Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor". Nature Methods. 3 (6). Springer Science and Business Media LLC: 455–460. doi:10.1038/nmeth885. ISSN 1548-7091. PMID 16721379. S2CID 10536176.
  13. ^ Tirlapur, Uday K.; König, Karsten (2002). "Targeted transfection by femtosecond laser". Nature. 418 (6895). Springer Science and Business Media LLC: 290–291. doi:10.1038/418290a. ISSN 0028-0836. PMID 12124612. S2CID 4370674.
  14. ^ Soughayer, Joseph S.; Krasieva, Tatiana; Jacobson, Stephen C.; Ramsey, J. Michael; Tromberg, Bruce J.; Allbritton, Nancy L. (2000). "Characterization of Cellular Optoporation with Distance". Analytical Chemistry. 72 (6). American Chemical Society (ACS): 1342–1347. doi:10.1021/ac990982u. ISSN 0003-2700. PMID 10740880. S2CID 6373605.
  15. ^ Mohanty, Samarendra K.; Sharma, Mrinalini; Gupta, Pradeep K. (2003). "Laser-assisted microinjection into targeted animal cells". Biotechnology Letters. 25 (11). Springer Science and Business Media LLC: 895–899. doi:10.1023/a:1024038609045. ISSN 0141-5492. PMID 12889802. S2CID 33912519.

and 25 Related for: Optical transfection information

Request time (Page generated in 0.8028 seconds.)

Optical transfection

Last Update:

Optical transfection is a biomedical technique that entails introducing nucleic acids (i.e. genetic material such as DNA) into cells using light. All cells...

Word Count : 2109

Transfection

Last Update:

Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. It may also refer to other methods and cell...

Word Count : 4151

Optogenetics

Last Update:

response to light, and in 2005 Zhuo-Hua Pan reported successful in-vivo transfection of channelrhodopsin in retinal ganglion cells of mice, and electrical...

Word Count : 11916

Biopolymer

Last Update:

applied for applications such as wound dressings, drug delivery and gene transfection. Starch: Starch is an inexpensive biodegradable biopolymer and copious...

Word Count : 3023

Potential applications of carbon nanotubes

Last Update:

first by binding their tips to cell membrane receptors. This enables transfection of molecular cargo attached to the CNT walls or encapsulated by CNTs...

Word Count : 15064

Bimolecular fluorescence complementation

Last Update:

fragments that had been fused to interacting proteins within 8 hours of transfection, reported in 2002. There are different production systems that can be...

Word Count : 5192

Green fluorescent protein

Last Update:

were 0.97. Another application is the use of GFP co-transfection as internal control for transfection efficiency in mammalian cells. A novel possible use...

Word Count : 7456

Calcium imaging

Last Update:

Calcium imaging is a microscopy technique to optically measure the calcium (Ca2+) status of an isolated cell, tissue or medium. Calcium imaging takes...

Word Count : 3884

Fluorophore

Last Update:

specific proteins to form a fusion protein, synthesized in cells after transfection of a suitable plasmid carrier. Non-protein organic fluorophores belong...

Word Count : 2024

Spherical nucleic acid

Last Update:

behavior, higher stability, and enhanced cellular uptake without the use of transfection agents (compared to the same sequence of linear DNA). It has been shown...

Word Count : 4215

Electroporation

Last Update:

process of introducing foreign DNA into eukaryotic cells is known as transfection. Electroporation is highly effective for transfecting cells in suspension...

Word Count : 5320

Nanoparticle drug delivery

Last Update:

Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency. Journal of Controlled Release, 2001. 70(3): p. 399-421. Pinto...

Word Count : 5293

Channelrhodopsin

Last Update:

in excitable cells such as neurons using a variety of transfection techniques (viral transfection, electroporation, gene gun) or transgenic animals. The...

Word Count : 5967

Transformation efficiency

Last Update:

high copy number origin of replication will generally have a higher transfection efficiency than one with a low copy number origin, using a plasmid with...

Word Count : 2952

Assay

Last Update:

in situ hybridization, combined to PCR, Computational genomics, and Transfection; Cell fractionation or Immunoprecipitation Migration assays, Chemotaxis...

Word Count : 3322

CRISPR gene editing

Last Update:

though it can result in harmful effects on the target cells. Chemical transfection techniques utilizing lipids and peptides have also been used to introduce...

Word Count : 17439

Mesoporous silica

Last Update:

Polyamidoamine Dendrimer-Capped Mesoporous Silica Nanosphere-Based Gene Transfection Reagent". Journal of the American Chemical Society. 126 (41): 13216–13217...

Word Count : 1976

Diatom

Last Update:

conjugation (with 10−6 and 10−4 yield respectively), and other classical transfection methods such as electroporation or use of PEG have been reported to provide...

Word Count : 15279

Ormosil

Last Update:

alternative to viral vectors for gene delivery, with higher transient transfection efficiencies suspension media and substrates for next generation solar...

Word Count : 509

Intracellular delivery

Last Update:

in the study of biology and genetics, such as the use of DNA plasmid transfection to investigate protein function in living cells. A wide range of approaches...

Word Count : 8788

Genetically modified organism

Last Update:

fish, zebrafish and medaka, are most commonly modified because they have optically clear chorions (membranes in the egg), rapidly develop, and the one-cell...

Word Count : 24587

Genetically modified animal

Last Update:

scientists were able to label cells for longer durations using a plasmid transfection technique, which involves injecting DNA into cells using an electrical...

Word Count : 11039

CRISPR

Last Update:

as failure of heart, hindrance of blood vessels, defects in growth and optical problems. To rehabilitate β-hemoglobinopathies, the patient's multipotent...

Word Count : 16261

GESTALT

Last Update:

field of research. CRISPR-Cas9 reagents can be introduced into cells via transfection using lipid nanoparticles. Alternatively, microinjection of the CRISPR-Cas9...

Word Count : 2706

Digital microfluidics

Last Update:

molecule PCR inside droplets. PCR amplified products are then validated by transfection into yeast cells and a Western blot protein identification. Problems...

Word Count : 10668
PDF Search Engine © AllGlobal.net