Nitrilase enzymes (nitrile aminohydrolase; EC 3.5.5.1) catalyse the hydrolysis of nitriles to carboxylic acids and ammonia, without the formation of "free" amide intermediates.[1] Nitrilases are involved in natural product biosynthesis and post translational modifications in plants, animals, fungi and certain prokaryotes. Nitrilases can also be used as catalysts in preparative organic chemistry. Among others, nitrilases have been used for the resolution of racemic mixtures. Nitrilase should not be confused with nitrile hydratase (nitrile hydro-lyase; EC 4.2.1.84) which hydrolyses nitriles to amides. Nitrile hydratases are almost invariably co-expressed with an amidase, which converts the amide to the carboxylic acid. Consequently, it can sometimes be difficult to distinguish nitrilase activity from nitrile hydratase plus amidase activity.
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Nitrilase enzymes (nitrile aminohydrolase; EC 3.5.5.1) catalyse the hydrolysis of nitriles to carboxylic acids and ammonia, without the formation of "free"...
In enzymology, a ricinine nitrilase (EC 3.5.5.2) is an enzyme that catalyzes the chemical reaction ricinine + 2 H2O ⇌ {\displaystyle \rightleftharpoons...
In enzymology, an aliphatic nitrilase also known as aliphatic nitrile aminohydrolase (EC 3.5.5.7) is an enzyme that catalyzes the hydrolysis of nitriles...
In enzymology, a bromoxynil nitrilase (EC 3.5.5.6) is an enzyme that catalyzes the chemical reaction 3,5-dibromo-4-hydroxybenzonitrile + 2 H2O ⇌ {\displaystyle...
In enzymology, a cyanoalanine nitrilase (EC 3.5.5.4) is an enzyme that catalyzes the chemical reaction 3-cyano-L-alanine + 2 H2O ⇌ {\displaystyle \rightleftharpoons...
oxysporum, and Aspergillus niger in which it induces production of the nitrilase enzyme. Buhler, D. R.; Reed, D. J. (2013). Nitrogen and Phosphorus Solvents...
from the genus Paenarthrobacter . Paenarthrobacter aurescens produces nitrilase and L-N-carbamoylase. Paenarthrobacter aurescens has a low GC-content...
NCC5H4N + 3 H2O Nicotinonitrile is a precursor to the vitamin niacin. Nitrilase-catalyzed hydrolysis of 3-cyanopyridine by means of immobilized Rhodococcus...
horticulture as a soil additive to improve plant growth. Additionally, a nitrilase enzyme from A. facilis has been engineered into E. coli for the commercial...
the pivotal intermediate R-3-Hydroxy-4-cyanobutyrate is now made with a nitrilase, and Merck & Co.'s Singulair (montelukast), where the reduction of a ketone...
RC(O)NH2}}} Two families of enzymes catalyze the hydrolysis of nitriles. Nitrilases hydrolyze nitriles to carboxylic acids: RCN + 2 H 2 O ⟶ RCO 2 H + NH 3...
doi:10.1007/s002510100327. PMID 11491533. Pace HC, Brenner C (2001). "The nitrilase superfamily: classification, structure and function". Genome Biol. 2 (1):...
JH, Huebner K, Buchberg AM, Mazo A, Brenner C, Croce CM (Aug 1998). "Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster...
source of nitrogen and carbon, and in concert act as an alternative to nitrilase activity, which performs nitrile hydrolysis without formation of an intermediate...
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