Metabolic flux analysis (MFA) is an experimental fluxomics technique used to examine production and consumption rates of metabolites in a biological system. At an intracellular level, it allows for the quantification of metabolic fluxes, thereby elucidating the central metabolism of the cell.[1] Various methods of MFA, including isotopically stationary metabolic flux analysis, isotopically non-stationary metabolic flux analysis, and thermodynamics-based metabolic flux analysis, can be coupled with stoichiometric models of metabolism and mass spectrometry methods with isotopic mass resolution to elucidate the transfer of moieties containing isotopic tracers from one metabolite into another and derive information about the metabolic network. Metabolic flux analysis (MFA) has many applications such as determining the limits on the ability of a biological system to produce a biochemical such as ethanol,[2] predicting the response to gene knockout,[3][4] and guiding the identification of bottleneck enzymes in metabolic networks for metabolic engineering efforts.[5]
Example of metabolic flux map for metabolic pathways of astrocytes and neurons.
Metabolic flux analysis may use 13C-labeled isotope tracers for isotopic labeling experiments. Nuclear magnetic resonance (NMR) techniques and mass spectrometry may then be used to measure metabolite labeling patterns to provide information for determination of pathway fluxes.[6][1][7] Because MFA typically requires rigorous flux calculation of complex metabolic networks, publicly available software tools have been developed to automate MFA and reduce its computational burden.
^Papoutsakis ET, Meyer CL (January 1985). "Equations and calculations of product yields and preferred pathways for butanediol and mixed-acid fermentations". Biotechnology and Bioengineering. 27 (1): 50–66. doi:10.1002/bit.260270108. PMID 18553576. S2CID 41031084.
^Burgard AP, Maranas CD (September 2001). "Probing the performance limits of the Escherichia coli metabolic network subject to gene additions or deletions". Biotechnology and Bioengineering. 74 (5): 364–375. doi:10.1002/bit.1127. PMID 11427938.
Metabolicfluxanalysis (MFA) is an experimental fluxomics technique used to examine production and consumption rates of metabolites in a biological system...
engineering to produce desired outputs. An analysis of metabolicflux can be found at Flux balance analysis The first step in the process is to identify...
Metabolicfluxanalysis (MFA) using stable isotope labeling is an important tool for explaining the flux of certain elements through the metabolic pathways...
method of flux balance analysis seeks to mathematically simulate metabolism in genome-scale reconstructions of metabolic networks. A metabolic reconstruction...
Flux balance analysis (FBA) is a mathematical method for simulating metabolism in genome-scale reconstructions of metabolic networks. In comparison to...
metabolism is a dynamic process. The significance of fluxomics is that metabolicfluxes determine the cellular phenotype. It has the added advantage of being...
properties and is a concept that relates to metabolicfluxanalysis. The concept was developed in 1999. In a metabolic cascade, many molecules will contain the...
Metabolic control analysis (MCA) is a mathematical framework for describing metabolic, signaling, and genetic pathways. MCA quantifies how variables,...
Material flow accounting Material flow analysis Medium-functioning autism, a classification of autism Metabolicfluxanalysis Methylfluoroalkoxy, a perfluoro...
PMID 8093448. Feng X, Tang KH, Blankenship RE, Tang YJ (December 2010). "Metabolicfluxanalysis of the mixotrophic metabolisms in the green sulfur bacterium Chlorobaculum...
effect on the flux of a metabolic pathway, then this enzyme is not involved in the control of the pathway. There are multiple levels of metabolic regulation...
Van Der Waals forces. The rate of turnover in a metabolic pathway, also known as the metabolicflux, is regulated based on the stoichiometric reaction...
demonstrate the correctness of his paper on metabolic control analysis. Kacser's paper with Jim Burns, The control of flux, later thoroughly revised to take account...
analyzed as a system, rather than a simple collection of parts, were Metabolic Control Analysis, developed by Henrik Kacser and Jim Burns later thoroughly revised...
general theory of metabolic control, which includes both metabolic control analysis and flux-oriented theory as special cases". The dynamics of a species...
support synthetic biology and metabolic engineering projects. Metabolomics Systems biology MetabolicfluxanalysisMetabolic engineering Synthetic biology...
considerable attention in online articles. Research on metabolic pathways includes flux balance analysis, which is used, for example, for explaining the Warburg...
MTT and other tetrazolium dyes depends on the cellular metabolic activity due to NAD(P)H flux. Cells with a low metabolism such as thymocytes and splenocytes...
hence the flux control coefficient on step one will be low. Branched pathways Control coefficient (biochemistry) Elasticity coefficient Metabolic control...
intracellular flux – the rate of metabolic activity – and use this information to regulate other metabolicfluxes, demonstrated that the intracellular flux state...
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