Wild-type Physcomitrella and knockout mosses: Deviating phenotypes induced in gene-disruption library transformants. Physcomitrella wild-type and transformed plants were grown on minimal Knop medium to induce differentiation and development of gametophores. For each plant, an overview (upper row; scale bar corresponds to 1 mm) and a close-up (bottom row; scale bar equals 0.5 mm) are shown. A: Haploid wild-type moss plant completely covered with leafy gametophores and close-up of wild-type leaf. B–E: Different mutants.[1]
A knockout moss is a kind of genetically modified moss. One or more of the moss's specific genes are deleted or inactivated ("knocked out"), for example by gene targeting or other methods. After the deletion of a gene, the knockout moss has lost the trait encoded by this gene. Thus, the function of this gene can be inferred. This scientific approach is called reverse genetics because the scientist wants to understand the function of a specific gene. In classical genetics, the scientist starts with a phenotype of interest and searches for the gene that causes this phenotype. Knockout mosses are relevant for basic research in biology as well as in biotechnology.
^Egener, Tanja; Granado, José; Guitton, Marie-Christine; Hohe, Annette; Holtorf, Hauke; Lucht, Jan M; Rensing, Stefan A; Schlink, Katja; Schulte, Julia; Schween, Gabriele; Zimmermann, Susanne; Duwenig, Elke; Rak, Bodo; Reski, Ralf (2002). "High frequency of phenotypic deviations in Physcomitrella patens plants transformed with a gene-disruption library". BMC Plant Biology. 2: 6. doi:10.1186/1471-2229-2-6. PMC 117800. PMID 12123528.
A knockoutmoss is a kind of genetically modified moss. One or more of the moss's specific genes are deleted or inactivated ("knocked out"), for example...
specific genomic position (a technique called gene targeting) to create knockoutmosses. This approach is called reverse genetics and it is a powerful and...
targeting (gene knockout) can be done in some organisms, such as yeast, mice and moss. Unique among plants, in Physcomitrella patens, gene knockout via homologous...
Gene knockouts (also known as gene deletion or gene inactivation) are a widely used genetic engineering technique that involves the targeted removal or...
Raw, Moss returned in a Raw Underground segment, an underground fight club hosted by Shane McMahon, defeating Cal Bloom by knockout. In October, Moss revealed...
Wild-type Physcomitrella and knockoutmosses: Deviating phenotypes induced in gene-disruption library transformants. Physcomitrella wild-type and transformed...
and mutant lines of Physcomitrella patens, including knockoutmosses. The long-term storage of moss samples in the IMSC is carried out via cryopreservation...
metabolism, and cell development of moss plants, using the technique of homologous recombination for creating knockoutmosses by gene targeting in a reverse...
genes in moss, the DNA is incubated together with freshly isolated protoplasts and with polyethylene glycol. As mosses are haploid organisms, moss filaments...
targeting that enables gene knockout. The resulting knockoutmosses are stored and distributed by the International Moss Stock Center. (Plant physiology...
fastest knockout in UFC history when his punches knocked down 410-lb Thomas Ramirez in just eight seconds. After a quick technical knockout of Sam Adkins...
Phil Moss (born 5 October 1971) is an English-born Australian association football manager and former player who was most recently assistant coach of Sydney...
Jordynne Grace's TNA Knockouts World Championship at Rebellion. At the event, De Lander unsuccessfully challenged Grace for the Knockouts Championship. Steph...
these as they did not consider themselves a Christian band. They soon became Moss Eisley, a reference to the Mos Eisley Cantina in the Star Wars trilogy. After...
best-known punch was a powerful left hook, which accounted for most of his knockouts. In his career, he lost to only two fighters, both former Olympic and...
group as leaders with 13 points in which they qualified to the knockout stage. In the knockout stage which is also known as the zonal semi-finals, the Mariners...
New York City. She has won major tournaments as Gail Shane, Gail Moss, and Gail Moss Greenberg. Sometime prior to the 2014 European and World meets (summer...
Yakup Saglam via second-round knockout. He maintained his unbeaten record, notching his fourteenth win and twelfth by knockout while retaining his two titles...
mammals, especially mice and rats, are used in experiments each year. A knockout mouse is a genetically modified mouse with an inactivated gene, replaced...
on September 27, Reigns retained the title against Jey Uso by technical knockout, after Reigns viciously beat down Jey, and Jimmy Uso threw in the towel...
undisclosed reasons. Verhoeven defeated the Hungarian by second-round knockout. He rematched and defeated Daniel Ghiță via unanimous decision in a bout...
providing the freedom to take on any type of persona. Continuing on the Knockout Mode which was introduced in EA Sports UFC 2, the game delivers a new type...
under new label Knockout Entertainment. It is a definitive and eclectic blend of a cappella Southern gospel songs. Norwood set up the KnockOut Vocal Studio...
Global Information