Global Information Lookup Global Information

Ion semiconductor sequencing information


Ion semiconductor sequencing is a method of DNA sequencing based on the detection of hydrogen ions that are released during the polymerization of DNA. This is a method of "sequencing by synthesis", during which a complementary strand is built based on the sequence of a template strand.

An Ion Proton semiconductor sequencer

A microwell containing a template DNA strand to be sequenced is flooded with a single species of deoxyribonucleotide triphosphate (dNTP). If the introduced dNTP is complementary to the leading template nucleotide, it is incorporated into the growing complementary strand. This causes the release of a hydrogen ion that triggers an ISFET ion sensor, which indicates that a reaction has occurred. If homopolymer repeats are present in the template sequence, multiple dNTP molecules will be incorporated in a single cycle. This leads to a corresponding number of released hydrogens and a proportionally higher electronic signal.

This technology differs from other sequencing-by-synthesis technologies in that no modified nucleotides or optics are used. Ion semiconductor sequencing may also be referred to as Ion Torrent sequencing, pH-mediated sequencing, silicon sequencing, or semiconductor sequencing.

and 23 Related for: Ion semiconductor sequencing information

Request time (Page generated in 0.7936 seconds.)

Ion semiconductor sequencing

Last Update:

Ion semiconductor sequencing is a method of DNA sequencing based on the detection of hydrogen ions that are released during the polymerization of DNA....

Word Count : 1466

Genomics

Last Update:

approach, ion semiconductor sequencing, is based on standard DNA replication chemistry. This technology measures the release of a hydrogen ion each time...

Word Count : 7539

DNA sequencing

Last Update:

standard sequencing chemistry, but with a novel, semiconductor-based detection system. This method of sequencing is based on the detection of hydrogen ions that...

Word Count : 14413

Amplicon

Last Update:

for DNA sequencing or next-generation sequencing, such as ion semiconductor sequencing, popularly referred to as the brand of the developer, Ion Torrent...

Word Count : 1380

Jonathan Rothberg

Last Update:

Bio-Rad Laboratories, Inc. Rothberg founded Ion Torrent in 2007, which developed ion semiconductor sequencing, a technology utilized by their Personal Genome...

Word Count : 2168

DNA sequencer

Last Update:

A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the...

Word Count : 3850

Cancer genome sequencing

Last Update:

Cancer Genome Consortium Ion semiconductor sequencing Nanopore sequencing Next-generation sequencing Oncogenomics Polony sequencing Precision medicine Pyrosequencing...

Word Count : 3085

Small RNA sequencing

Last Update:

approaches: Ion Torrent sequencing: NGS technology based on the use of a semiconductor chip where the sample is loaded integrated with an ion-sensitive...

Word Count : 2056

ISFET

Last Update:

substrate and oxide surfaces arises due to an ion sheath. It is a special type of MOSFET (metal–oxide–semiconductor field-effect transistor), and shares the...

Word Count : 2278

List of semiconductor scale examples

Last Update:

are many semiconductor scale examples for various metal–oxide–semiconductor field-effect transistor (MOSFET, or MOS transistor) semiconductor manufacturing...

Word Count : 5940

Gordon Moore

Last Update:

; Miao, X.; Reed, B. (2011). "An integrated semiconductor device enabling non-optical genome sequencing". Nature. 475 (7356): 348–352. doi:10.1038/nature10242...

Word Count : 3265

Laser

Last Update:

are used in optical disc drives, laser printers, barcode scanners, DNA sequencing instruments, fiber-optic, and free-space optical communication, semiconducting...

Word Count : 12666

Sensor

Last Update:

PMID 27065784. "1960: Metal Oxide Semiconductor (MOS) Transistor Demonstrated". The Silicon Engine: A Timeline of Semiconductors in Computers. Computer History...

Word Count : 3169

List of MOSFET applications

Last Update:

The MOSFET (metal–oxide–semiconductor field-effect transistor) is a type of insulated-gate field-effect transistor (IGFET) that is fabricated by the controlled...

Word Count : 14390

Lanthanide

Last Update:

instead of lanthanide, as the ending -ide normally indicates a negative ion. However, owing to widespread current use, lanthanide is still allowed. v...

Word Count : 10251

Piet Bergveld

Last Update:

development of the ion-sensitive field-effect transistor (ISFET) sensor. It was based on his earlier research on the MOSFET (metal–oxide–semiconductor field-effect...

Word Count : 552

Nanomaterials

Last Update:

Size-dependent properties are observed such as quantum confinement in semiconductor particles, surface plasmon resonance in some metal particles, and superparamagnetism...

Word Count : 10264

Plasma cleaning

Last Update:

light blue color. A plasma’s activated species include atoms, molecules, ions, electrons, free radicals, metastables, and photons in the short wave ultraviolet...

Word Count : 2481

Cees Dekker

Last Update:

research on magnetic spin systems and on noise in superconductors and semiconductors. In 1993 he was appointed as Associate Professor at Delft University...

Word Count : 1775

Biosensor

Last Update:

DNA sequencing". Electronics Letters. 47: S7–S12. doi:10.1049/el.2011.3231. Retrieved 13 May 2016. Bergveld, P. (January 1970). "Development of an Ion-Sensitive...

Word Count : 12037

Automated patch clamp

Last Update:

transcriptomic properties of neurons at the scale comparable to other sequencing methods The automation technique varies, depending on the surrounding...

Word Count : 2412

Power supply

Last Update:

including automated equipment testing, crystal growth monitoring, semiconductor fabrication, and x-ray generators. Programmable power supplies typically...

Word Count : 3905

Potential applications of graphene

Last Update:

potentially solve a bottleneck for nanopore-based single-molecule DNA sequencing. On November 20, 2013, the Bill & Melinda Gates Foundation awarded $100...

Word Count : 15289

PDF Search Engine © AllGlobal.net