Ion semiconductor sequencing is a method of DNA sequencing based on the detection of hydrogen ions that are released during the polymerization of DNA. This is a method of "sequencing by synthesis", during which a complementary strand is built based on the sequence of a template strand.
An Ion Proton semiconductor sequencer
A microwell containing a template DNA strand to be sequenced is flooded with a single species of deoxyribonucleotide triphosphate (dNTP). If the introduced dNTP is complementary to the leading template nucleotide, it is incorporated into the growing complementary strand. This causes the release of a hydrogen ion that triggers an ISFET ion sensor, which indicates that a reaction has occurred. If homopolymer repeats are present in the template sequence, multiple dNTP molecules will be incorporated in a single cycle. This leads to a corresponding number of released hydrogens and a proportionally higher electronic signal.
This technology differs from other sequencing-by-synthesis technologies in that no modified nucleotides or optics are used. Ion semiconductor sequencing may also be referred to as Ion Torrent sequencing, pH-mediated sequencing, silicon sequencing, or semiconductor sequencing.
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Ionsemiconductorsequencing is a method of DNA sequencing based on the detection of hydrogen ions that are released during the polymerization of DNA....
approach, ionsemiconductorsequencing, is based on standard DNA replication chemistry. This technology measures the release of a hydrogen ion each time...
standard sequencing chemistry, but with a novel, semiconductor-based detection system. This method of sequencing is based on the detection of hydrogen ions that...
for DNA sequencing or next-generation sequencing, such as ionsemiconductorsequencing, popularly referred to as the brand of the developer, Ion Torrent...
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A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the...
approaches: Ion Torrent sequencing: NGS technology based on the use of a semiconductor chip where the sample is loaded integrated with an ion-sensitive...
substrate and oxide surfaces arises due to an ion sheath. It is a special type of MOSFET (metal–oxide–semiconductor field-effect transistor), and shares the...
are many semiconductor scale examples for various metal–oxide–semiconductor field-effect transistor (MOSFET, or MOS transistor) semiconductor manufacturing...
are used in optical disc drives, laser printers, barcode scanners, DNA sequencing instruments, fiber-optic, and free-space optical communication, semiconducting...
PMID 27065784. "1960: Metal Oxide Semiconductor (MOS) Transistor Demonstrated". The Silicon Engine: A Timeline of Semiconductors in Computers. Computer History...
The MOSFET (metal–oxide–semiconductor field-effect transistor) is a type of insulated-gate field-effect transistor (IGFET) that is fabricated by the controlled...
instead of lanthanide, as the ending -ide normally indicates a negative ion. However, owing to widespread current use, lanthanide is still allowed. v...
development of the ion-sensitive field-effect transistor (ISFET) sensor. It was based on his earlier research on the MOSFET (metal–oxide–semiconductor field-effect...
Size-dependent properties are observed such as quantum confinement in semiconductor particles, surface plasmon resonance in some metal particles, and superparamagnetism...
light blue color. A plasma’s activated species include atoms, molecules, ions, electrons, free radicals, metastables, and photons in the short wave ultraviolet...
research on magnetic spin systems and on noise in superconductors and semiconductors. In 1993 he was appointed as Associate Professor at Delft University...
DNA sequencing". Electronics Letters. 47: S7–S12. doi:10.1049/el.2011.3231. Retrieved 13 May 2016. Bergveld, P. (January 1970). "Development of an Ion-Sensitive...
transcriptomic properties of neurons at the scale comparable to other sequencing methods The automation technique varies, depending on the surrounding...
potentially solve a bottleneck for nanopore-based single-molecule DNA sequencing. On November 20, 2013, the Bill & Melinda Gates Foundation awarded $100...
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