Ion semiconductor sequencing is a method of DNA sequencing based on the detection of hydrogen ions that are released during the polymerization of DNA. This is a method of "sequencing by synthesis", during which a complementary strand is built based on the sequence of a template strand.
An Ion Proton semiconductor sequencer
A microwell containing a template DNA strand to be sequenced is flooded with a single species of deoxyribonucleotide triphosphate (dNTP). If the introduced dNTP is complementary to the leading template nucleotide, it is incorporated into the growing complementary strand. This causes the release of a hydrogen ion that triggers an ISFET ion sensor, which indicates that a reaction has occurred. If homopolymer repeats are present in the template sequence, multiple dNTP molecules will be incorporated in a single cycle. This leads to a corresponding number of released hydrogens and a proportionally higher electronic signal.
This technology differs from other sequencing-by-synthesis technologies in that no modified nucleotides or optics are used. Ion semiconductor sequencing may also be referred to as Ion Torrent sequencing, pH-mediated sequencing, silicon sequencing, or semiconductor sequencing.
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Ionsemiconductorsequencing is a method of DNA sequencing based on the detection of hydrogen ions that are released during the polymerization of DNA....
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standard sequencing chemistry, but with a novel, semiconductor-based detection system. This method of sequencing is based on the detection of hydrogen ions that...
for DNA sequencing or next-generation sequencing, such as ionsemiconductorsequencing, popularly referred to as the brand of the developer, Ion Torrent...
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substrate and oxide surfaces arises due to an ion sheath. It is a special type of MOSFET (metal–oxide–semiconductor field-effect transistor), and shares the...
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Size-dependent properties are observed such as quantum confinement in semiconductor particles, surface plasmon resonance in some metal particles, and superparamagnetism...
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DNA sequencing". Electronics Letters. 47: S7–S12. doi:10.1049/el.2011.3231. Retrieved 13 May 2016. Bergveld, P. (January 1970). "Development of an Ion-Sensitive...
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transcriptomic properties of neurons at the scale comparable to other sequencing methods The automation technique varies, depending on the surrounding...
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