Principle of interference reflection microscopy (IRM) showing interference effect on reflected waves and the result on the final image intensity. Dark purple wave represents the light from the light source. The light purple waves are the reflections from the cell membrane and from the glass surface. Upon hitting the glass surface, the reflected waves are shifted half a wavelength. When the membrane is very close to the glass, the reflected light will be reflected out of phase with the reflected beam from the glass. This will cause destructive interference (see red line), resulting in a dark pixel. If there is more distance between the membrane and the glass, the returning waves will be less shifted and will cause constructive interference (see red line), resulting in a brighter pixel in the final image. Indicated are the typical refractive indices of the glass, medium and the cell membrane, which determine the amount of reflection.
Interference reflection microscopy (IRM), also called Reflection Interference Contrast Microscopy (RICM) or Reflection Contrast Microscopy (RCM) depending on the context, is an optical microscopy technique that leverages interference effects to form an image of an object on a glass surface. The intensity of the signal is a measure of proximity of the object to the glass surface. This technique can be used to study events at the cell membrane without the use of a (fluorescent) label as is the case for TIRF microscopy.
and 24 Related for: Interference reflection microscopy information
Interferencereflectionmicroscopy (IRM), also called ReflectionInterference Contrast Microscopy (RICM) or Reflection Contrast Microscopy (RCM) depending...
complex microscopy techniques which take advantage of polarized light include differential interference contrast microscopy and interferencereflection microscopy...
An additional technique using interference is interferencereflectionmicroscopy (also known as reflected interference contrast, or RIC). It relies on...
contribution to the field of fluorescence microscopy, and invented reflectioninterference contrast microscopy. Ploem was born on 25 August 1927 in Sawahlunto...
the Canadian Space Agency Reflection Contrast Microscopy, or Interferencereflectionmicroscopy, a type of optical microscopy Regulatory Compliance Mark...
eugenol L'Institut Royal Météorologique de Belgique Interferencereflectionmicroscopy, a microscopy method used to image adherent cells Internal Revenue...
Transmission electron microscopy (TEM) where swift electrons go through a thin sample Scanning transmission electron microscopy (STEM) which is similar...
same principle, but focus exclusively on reflection. Dichroic filters use the principle of thin-film interference, and produce colors in the same way as...
backscattering spectrometry REM – Reflection electron microscopy RDS – Reflectance difference spectroscopy RHEED – Reflection high energy electron diffraction...
improved the technique of reflectioninterference contrast microscopy – RICM (which is quantitative interferencereflectionmicroscopy – IRM) – a powerful tool...
Fluorescence interference contrast (FLIC) microscopy is a microscopic technique developed to achieve z-resolution on the nanometer scale. FLIC occurs...
Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique...
theory for differential interference contrast microscopy, another interference-based imaging technique. Modern biological microscopy depends heavily on the...
holographic microscopy (DHM) is digital holography applied to microscopy. Digital holographic microscopy distinguishes itself from other microscopy methods...
intensity by interference with a reference beam. In the visual spectrum this is done using Zernike phase-contrast microscopy, differential interference contrast...
methods such as phase contrast or differential interference contrast, or reflectioninterferencemicroscopy. The key feature of iSCAT is the detection of...
microscopy, and interferometry. In principle, it is possible to make a hologram for any type of wave. A hologram is a recording of an interference pattern...
RICM may refer to: ReflectionInterference Contrast Microscopy, a type of optical microscopy Régiment d'infanterie-chars de marine, a regiment of the...
Acoustic microscopy is microscopy that employs very high or ultra high frequency ultrasound. Acoustic microscopes operate non-destructively and penetrate...
Photo-activated localization microscopy (PALM or FPALM) and stochastic optical reconstruction microscopy (STORM) are widefield (as opposed to point scanning...
scattering dominates, in particular for electron diffraction and electron microscopy, X-ray diffraction and neutron diffraction. In these the relevant waves...
biological cells or single protein and DNA molecules for microscopy (as in the total internal reflection fluorescence microscope). The evanescent wave from...
for light microscopy. Glass knives are used to slice sections for light microscopy and to slice very thin sections for electron microscopy. Industrial...
Global Information