Typical iSCAT configurations where either the reflected light from the cover-slip (a, c) or the transmitted light through the sample (b, d) is used as a reference field. The signal can be acquired with a camera in wide-field operation (a, b) or by point detection in confocal arrangement (c, d).
Interferometric scattering microscopy (iSCAT) refers to a class of methods that detect and image a subwavelength object by interfering the light scattered by it with a reference light field. The underlying physics is shared by other conventional interferometric methods such as phase contrast or differential interference contrast, or reflection interference microscopy. The key feature of iSCAT is the detection of elastic scattering from subwavelength particles, also known as Rayleigh scattering, in addition to reflected or transmission signals from supra-wavelength objects. Typically, the challenge is the detection of tiny signals on top of large and complex, speckle-like backgrounds. iSCAT has been used to investigate nanoparticles such as viruses, proteins, lipid vesicles, DNA, exosomes, metal nanoparticles, semiconductor quantum dots, charge carriers and single organic molecules without the need for a fluorescent label.
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Interferometricscatteringmicroscopy (iSCAT) refers to a class of methods that detect and image a subwavelength object by interfering the light scattered...
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Interferometric nanoparticle tracking analysis (iNTA) is the next generation of NTA technology. It is based on interferometricscatteringmicroscopy (iSCAT)...
contributions to femtosecond stimulated Raman spectroscopy (FSRS), interferometricscatteringmicroscopy (iSCAT) and the development of mass photometry. He was born...
technology is called interferometric nanoparticle tracking analysis (iNTA) and is based on the interferometricscatteringmicroscopy (iSCAT). In contrast...
spring constants. Scattering techniques: single particle tracking based on dark field microscopy or interferometricscatteringmicroscopy (iSCAT) Single-molecule...
interference contrast (DIC) microscopy, also known as Nomarski interference contrast (NIC) or Nomarski microscopy, is an optical microscopy technique used to enhance...
interference contrast microscopy (FLIC) and reflection interference contrast microscopy (RICM) or interferometricscatteringmicroscopy (iSCAT). When the...
the single-molecule level. The technique is based on interferometric scattered light microscopy. Contrast from scattered light by a single binding event...
of optical microscopy. The corresponding technique is called interferometricmicroscopy. Recent achievements of interferometricmicroscopy allow one to...
remote-focusing interferometricscattering (RF-iSCAT) microscopy, to track a nanoparticle motion along the beam propagation direction. Then, this interferometric scattering...
measured at various illumination angles, employing the principle of interferometric imaging. Then, a 3D RI tomogram of the sample is reconstructed from...
i.e. like images of classical microscopy: orthogonal to the light beam of illumination. More precisely, interferometric images are created by a Michelson...
similar to a standard optical microscope. The only differences are an interferometric objective lens and an accurate positioning stage (a piezoelectric actuator)...
studies led to a new application area for digital holographic and interferometricmicroscopy. In September 2009, Choi returned to South Korea to work in the...
of the 1953 Nobel Prize in Physics and the inventor of phase-contrast microscopy, they play important roles in various optics branches such as beam optics...
novel sensing methods, such as fluorescent glucose biosensors. The interferometric reflectance imaging sensor (IRIS) is based on the principles of optical...
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