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Crystal structure of I-CreI bound to its DNA recognition sequence. The enzyme binds as a homodimer; one subunit is depicted in yellow, the other in pink. The enzyme is shown in surface representation; DNA molecule is shown as a collection of spheres, each colored according to its chemical element.
The homing endonucleases are a collection of endonucleases encoded either as freestanding genes within introns, as fusions with host proteins, or as self-splicing inteins. They catalyze the hydrolysis of genomic DNA within the cells that synthesize them, but do so at very few, or even singular, locations. Repair of the hydrolyzed DNA by the host cell frequently results in the gene encoding the homing endonuclease having been copied into the cleavage site, hence the term 'homing' to describe the movement of these genes. Homing endonucleases can thereby transmit their genes horizontally within a host population, increasing their allele frequency at greater than Mendelian rates.
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The homingendonucleases are a collection of endonucleases encoded either as freestanding genes within introns, as fusions with host proteins, or as self-splicing...
homozygote bearing a copy of the homingendonuclease on both homologous chromosomes. This gives homingendonucleases an allele frequency dynamics rather...
restriction enzyme HindIII – A restriction enzyme Homingendonuclease List of homingendonuclease cutting sites List of restriction enzyme cutting sites...
The homingendonucleases are a special type of restriction enzymes encoded by introns or inteins. They act on the cellular DNA of the cell that synthesizes...
restriction enzymes. Among meganucleases, the LAGLIDADG family of homingendonucleases has become a valuable tool for the study of genomes and genome engineering...
splicing and usually contain endonuclease and splicing activities. The allele without the HEGs are cleaved by the homingendonuclease and the double-strand break...
"target sequence", i.e. the rival allele. This can be one of: A homingendonuclease, which is what natural inteins use to propagate. They are, however...
bacteria. One special kind of restriction enzymes is the class of "homingendonucleases", these being present in all three domains of life, although their...
"Efficient targeting of a SCID gene by an engineered single-chain homingendonuclease". Nucleic Acids Research. 37 (16): 5405–19. doi:10.1093/nar/gkp548...
DNA endonuclease, as was predicted by the model 12 years ago. This nuclease took afterwards the conventional name of I-SceI, the first intron homing endonuclease...
types of endonucleases for DNA assembly. For example, both the iBrick standard and the HomeRun vector assembly standards employ homingendonucleases instead...
"Efficient targeting of a SCID gene by an engineered single-chain homingendonuclease". Nucleic Acids Research. 37 (16): 5405–19. doi:10.1093/nar/gkp548...
Airport, Jacksonville, Florida, US (FAA LID:HEG) Hemoencephalography Homingendonuclease genes Homogeneous electron gas Host Europe Group, a web hosting company...
double strand DNA breaks in the genome, which is typical of other homingendonuclease mediated genome editing methods. Users can design a rAAV vector to...
Windbichler et al., 2007 and Galizi et al., 2014 (a Physarum polycephalum homingendonuclease which destroys X chromosomes), Windbichler et al., 2008 and Hammond...
catalyzes a type of site-specific homologous recombination I-CreI, a homingendonuclease CRE, LRT station abbreviation for Coral Edge LRT station, Singapore...
encoded by the DNA of a gene. Additionally, most inteins contain a homingendonuclease or HEG domain which is capable of finding a copy of the parent gene...
encoded homingendonuclease. The mS1247 twintron encod ORF have been biochemically characterized and the results showed that it is an active homing endonuclease...
explaining the origin of group I introns. Some group I introns encode homingendonuclease (HEG), which catalyzes intron mobility. It is proposed that HEGs...
from Glomeromycota. Many of the newly described inteins contain homingendonucleases and some of these are apparently active. The abundance of intein...
"Efficient targeting of a SCID gene by an engineered single-chain homingendonuclease". Nucleic Acids Research. 37 (16): 5405–19. doi:10.1093/nar/gkp548...
enhanced through the use of site-specific endonucleases such as zinc finger nucleases, engineered homingendonucleases, TALENS, or most commonly the CRISPR-Cas...
"Efficient targeting of a SCID gene by an engineered single-chain homingendonuclease". Nucleic Acids Research. 37 (16): 5405–19. doi:10.1093/nar/gkp548...
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