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1.The insertion sequences (yellow) on both the F factor plasmid and the chromosome have similar sequences, allowing the F factor to insert itself into the genome of the cell. This is called homologous recombination and creates an Hfr (high frequency of recombination) cell. 2.The Hfr cell forms sex pili a pilus and attaches to a recipient F- cell. 3.A nick in one strand of the Hfr cell’s chromosome is created. 4.DNA begins to be transferred from the Hfr cell to the recipient cell while the second strand of its chromosome is being replicated. 5.The pilus detaches from the recipient cell and retracts. The Hfr cell ideally wants to transfer its entire genome to the recipient cell. However, due to its large size and inability to keep in contact with the recipient cell, it is not able to do so. 6.The F- cell remains F- because the entire F factor sequence was not received. Since no homologous recombination occurred, the DNA that was transferred is degraded by enzymes. In very rare cases, the F factor will be completely transferred and the F- cell will become an Hfr cell. [1]
A high-frequency recombination cell (Hfr cell) (also called an Hfr strain) is a bacterium with a conjugative plasmid (for example, the F-factor) integrated into its chromosomal DNA. The integration of the plasmid into the cell's chromosome is through homologous recombination. A conjugative plasmid capable of chromosome integration is also called an episome (a segment of DNA that can exist as a plasmid or become integrated into the chromosome). When conjugation occurs, Hfr cells are very efficient in delivering chromosomal genes of the cell into recipient F− cells, which lack the episome.
A high-frequency recombination cell (Hfrcell) (also called an Hfr strain) is a bacterium with a conjugative plasmid (for example, the F-factor) integrated...
Look up HFR in Wiktionary, the free dictionary. HFR or Hfr may refer to: High frame rate, in motion pictures Hfrcell, or Hfr strain, a bacterium with...
called Hfr (high frequency of recombination). The E. coli genome was originally mapped by interrupted mating experiments in which various Hfrcells in the...
transfer of genetic material of an Hfrcell to an F- cell through conjugation. When an Hfrcell mates with an F- cell, the chromosome acts as a rolling...
in a certain order from the Hfr donor cell to the F− recipient cell during mating. The longer that the Hfr and F− cells were in contact, the more genes...
Hershey–Chase experiment – heterochromatin – heterotroph – heterozygote – Hfrcell – hibernation – hierarchy of life – Hill reaction – His tag – histone –...
that is involved in the mating of prokaryotic organisms. Transferred DNA of Hfr (high frequency of recombination) is called exogenote and homologous part...
diseases in humans, such as hantavirus hemorrhagic fever with renal syndrome (HFRS), or hantavirus pulmonary syndrome (HPS), also known as hantavirus cardiopulmonary...
conjugation (Hfr), mycobacterial conjugation is a type of HGT that is chromosome rather than plasmid based. Furthermore, in contrast to E. coli (Hfr) conjugation...
homologous recombination. However, in contrast to the well-known E. coli Hfr conjugation system, in M. smegmatis all regions of the chromosome are transferred...
ionic equilibrium within the cell. Alpha toxin binds GPI-anchored cell surface receptors including the human folate receptor (hFR) as well as the neuronal...
causative agents of Hantavirus hemorrhagic fever with renal syndrome (HV-HFRS) (Hantaviridae), the Crimean-Congo hemorrhagic fever (CCHF) virus from the...
ejection fraction, called heart failure with reduced ejection fraction (HFrEF). Risk factors for HFpEF include hypertension, hyperlipidemia, diabetes...
Finland; the RA-6 CNEA reactor in Bariloche, Argentina; the High Flux Reactor (HFR) at Petten in the Netherlands; and Tsing Hua Open-pool Reactor (THOR) at...
Vericiguat in Participants With Heart Failure With Reduced Ejection Fraction (HFrEF) (MK-1242-001) (VICTORIA)" at ClinicalTrials.gov Portal: Medicine...
folate receptor (hFR) mRNAs originate from two tissue-specific promoters and alternative splicing: characterization of the alpha hFR gene structure"....
of Acanthamoeba Granulomatous Amebic Encephalitis in a Hematopoietic Stem Cell Transplant Recipient". American Journal of Case Reports. 21: e923219. doi:10...
2001). "Regression of Human T-cell Leukemia Virus Type I (HTLV-I)-Associated Lymphomas in a Rat Model: Peptide-Induced T-Cell Immunity". Journal of the National...
broadcasts and 1080p mobile broadcasts via the VVC codec, including HDR and HFR) is currently under development. Japan Philippines (officially adopted...
fraction (HFrEF): Synonyms no longer recommended are "heart failure due to left ventricular systolic dysfunction" and "systolic heart failure". HFrEF is associated...
contraindicated in patients with heart failure with reduced ejection fraction (HFrEF), and second- or third-degree atrioventricular block. Special attention...