Comparison of resolution between standard confocal microscopy and GSD microscopy. Left: Confocal recording of vacancies in diamonds. Single spots cannot be separated. Right: GSD recording of the same location. Single vacancies are clearly visible. The size of the pointlike vacancies, corresponding to the microscope's resolution, is about 15 nm.
Ground state depletion microscopy (GSD microscopy) is an implementation of the RESOLFT concept. The method was proposed in 1995[1] and experimentally demonstrated in 2007.[2] It is the second concept to overcome the diffraction barrier in far-field optical microscopy published by Stefan Hell. Using nitrogen-vacancy centers in diamonds a resolution of up to 7.8 nm was achieved in 2009.[3] This is far below the diffraction limit (~200 nm).
^Stefan W. Hell M. Kroug (1995). "Ground-state-depletion fluorescence microscopy: a concept for breaking the diffraction resolution limit". Applied Physics B: Lasers and Optics. 60 (5): 495–497. Bibcode:1995ApPhB..60..495H. doi:10.1007/BF01081333.
^Stefan Bretschneider; Christian Eggeling; Stefan W. Hell (2007). "Breaking the diffraction barrier in fluorescence microscopy by optical shelving". Physical Review Letters. 98 (5): 218103. Bibcode:2007PhRvL..98u8103B. doi:10.1103/PhysRevLett.98.218103. hdl:11858/00-001M-0000-0012-E125-B. PMID 17677813.
^Eva Rittweger; Dominik Wildanger; Stefan W. Hell (2009). "Far-field fluorescence nanoscopy of diamond color centers by ground state depletion" (PDF). EPL. 86 (1): 14001. Bibcode:2009EL.....8614001R. doi:10.1209/0295-5075/86/14001.
Ground state depletion microscopy (GSDmicroscopy) is an implementation of the RESOLFT concept. The method was proposed in 1995 and experimentally demonstrated...
imaged with conventional or confocal microscopy. Within RESOLFT the principles of STED microscopy and GSDmicroscopy are generalized. Structures that are...
Chemistry in 2014 "for the development of super-resolved fluorescence microscopy", together with Eric Betzig and William Moerner. Born into a Roman Catholic...
phosphate (GSD Ic) or glucose (GSD Id); however, a recent study suggests that the biochemical assays used to differentiate GSD Ic and GSD Id from GSD Ib are...
Glycogen storage disease type V (GSD5, GSD-V), also known as McArdle's disease, is a metabolic disorder, one of the metabolic myopathies, more specifically...
imperfect focus, and atmospheric distortion. The ground sample distance (GSD) of an image, the pixel spacing on the Earth's surface, is typically considerably...
lichen in Cajas National Park. Passage translated from Latin by GPT-4. "GSD Species Synonymy. Current Name: Ramalina peruviana Ach., Lich. Univ.: 599...
identification and control. Though reliable, DNA hybridization, electron microscopy and PCR techniques require specialized equipment and trained human resources...
genes are the cause of glycogen storage disease type IX (GSD type IX) and GSD type VI (formerly GSD type VIII), which can affect the liver and/or muscles...
is a meta-database of about 150 specialized "global species databases" (GSDs) that have collected the names and other information on (almost) all described...
similar habitats. List of Agaricales genera List of Agaricaceae genera "GSD Species Synonymy: Calbovista subsculpta Morse ex M.T. Seidl". Species Fungorum...
in Europe, North America, Costa Rica, South America (Brazil), and Korea. "GSD Species Synonymy: Loweomyces fractipes (Berk. & M.A. Curtis) Jülich". Species...
Global Information