RESOLFT, an acronym for REversible Saturable OpticaLFluorescence Transitions, denotes a group of optical fluorescence microscopy techniques with very high resolution. Using standard far field visible light optics a resolution far below the diffraction limit down to molecular scales can be obtained.
With conventional microscopy techniques, it is not possible to distinguish features that are located at distances less than about half the wavelength used (i.e. about 200 nm for visible light). This diffraction limit is based on the wave nature of light. In conventional microscopes the limit is determined by the used wavelength and the numerical aperture of the optical system. The RESOLFT concept surmounts this limit by temporarily switching the molecules to a state in which they cannot send a (fluorescence-) signal upon illumination. This concept is different from for example electron microscopy where instead the used wavelength is much smaller.
RESOLFT, an acronym for REversible Saturable OpticaL Fluorescence Transitions, denotes a group of optical fluorescence microscopy techniques with very...
contributions to advanced microscopy, particularly superresolution microscopy (RESOLFT, STED).[citation needed] Testa studied physics at University of Genoa in...
Alma mater Heidelberg University Occupation Physicist Known for STED microscopy RESOLFT GSD microscopy 4Pi microscope Multifocal multiphoton microscopy Three photon...
microscopy, proposed in 1994. This method and all techniques following the RESOLFT concept rely on a strong non-linear interaction between light and fluorescing...
state depletion microscopy (GSD microscopy) is an implementation of the RESOLFT concept. The method was proposed in 1995 and experimentally demonstrated...
of 150–200 in fixed cells. With the combination of 4Pi microscopy and RESOLFT microscopy with switchable proteins, it is now possible to take images...
techniques, including stimulated emission depletion (STED) and its related RESOLFT (reversible saturable optically linear fluorescent transitions) microscopy;...
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