Restriction endonuclease Fok1 bound to DNA PDB 1fok[1]
Identifiers
Symbol
Endonuc-Fok1_C
Pfam
PF09254
Pfam clan
CL0415
InterPro
IPR015334
SCOP2
2fok / SCOPe / SUPFAM
Available protein structures:
Pfam
structures / ECOD
PDB
RCSB PDB; PDBe; PDBj
PDBsum
structure summary
The restriction endonuclease Fok1, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non sequence-specific DNA cleavage domain at the C-terminal.[2] Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3' recognition site, the DNA cleavage domain is activated and cleaves the DNA at two locations, regardless of the nucleotide sequence at the cut site. The DNA is cut 9 nucleotides downstream of the motif on the forward strand, and 13 nucleotides downstream of the motif on the reverse strand,[3] producing two sticky ends with 4-bp overhangs.
Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
^Aggarwal, A. K.; Wah, D. A.; Hirsch, J. A.; Dorner, L. F.; Schildkraut, I. (1997). "Structure of the multimodular endonuclease Fok1 bound to DNA". Nature. 388 (6637): 97–100. doi:10.1038/40446. PMID 9214510. S2CID 205027830.
^Durai S, Mani M, Kandavelou K, Wu J, Porteus M, Chandrasegaran S (2005). "Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells". Nucleic Acids Res. 33 (18): 5978–90. doi:10.1093/nar/gki912. PMC 1270952. PMID 16251401.
^Wah, D. A.; Bitinaite, J.; Schildkraut, I.; Aggarwal, A. K. (1998). "Structure of Fok1 has implications for DNA cleavage". Proc Natl Acad Sci USA. 95 (18): 10564–9. Bibcode:1998PNAS...9510564W. doi:10.1073/pnas.95.18.10564. PMC 27934. PMID 9724743.
Aggarwal, A. K.; Wah, D. A.; Hirsch, J. A.; Dorner, L. F.; Schildkraut, I. (1997). "Structure of the multimodular endonuclease Fok1 bound to DNA". Nature...
double-strand breaks (DSBs) in target DNA by the restriction endonucleases (FokI and Cas), and the repair of DSBs through homology-directed recombination...
Foki (Russian: Фоки) is a rural locality (a selo) and the administrative center of Fokinskoye Rural Settlement, Chaykovsky, Perm Krai, Russia. The population...
restriction endonucleases, such as DpnI, are able to recognize and cut methylated DNA. Type IIS restriction endonucleases (e.g. FokI) cleave DNA at a defined distance...
also requires transition molecules as well as the FokI enzyme to be present. The requirement for the FokI enzyme limits application in vivo, at least for...
okeanokoites is the original source for the type IIs restriction endonuclease FokI, used in Zinc finger nucleases and TALENs. Species who are a part of the...
Timothy Fok Tsun-ting (born 14 February 1946), GBS, JP, the eldest son of Henry Fok, is the President of the Hong Kong Football Association. He formerly...
monomers in which each subunit contains a zinc domain and a FokI endonuclease domain. The FokI domains must dimerize for activities, thus narrowing target...
cleavage domain (usually the cleavage domain of FokI) to generate zinc finger nucleases. Such zinc finger-FokI fusions have become useful reagents for manipulating...
and VDR association with progression to AIDS follows an additive model. FokI polymorphism is a risk factor for enveloped virus infection as revealed in...
are typically created through the fusion of Zinc-finger domains and the FokI nuclease domain. ZFNs have thus the ability to cleave DNA at target sites...
made þaim kaiba i bormoþa huni A2 huwaʀ ob kam harie a hit lat A3 gotna fiskʀ oʀ firnauim suwimade foki af (f)a(nwan)ga lande B a(i a)u is urki C1 ni...
PMID 15458627. Bitinaite J, Wah DA, Aggarwal AK, Schildkraut I (September 1998). "FokI dimerization is required for DNA cleavage". Proceedings of the...
is the creation of artificial restriction enzymes created by linking the FokI DNA cleavage domain with an array of DNA binding proteins or zinc finger...
another nuclease enzyme from E. coli. FokI, a nuclease enzyme from Flavobacterium okeanokoites Schildkraut I, Banner CD, Rhodes CS, Parekh S (1984)....
pluripotency in human and mouse cells providing an alternative method to iPS technology. In addition, large-scale activation screens could be used to...
subsequent infections. Hence these sequences play a key role in the antiviral (i.e. anti-phage) defense system of prokaryotes and provide a form of acquired...
April 19, 2022. Retrieved May 22, 2023. "Tiafoe Gets Down & Dirty To Upset Foki In Estoril". www.atptour.com. April 29, 2022. Retrieved May 22, 2023. Levinsohn...
originates, this guide RNA is a copy of a piece of foreign nucleic acid (i.e. phage) that previously infected the cell. It is of interest to researchers...
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