Firefly luciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme catalyses the oxidation of firefly luciferin, requiring oxygen and ATP. Because of the requirement of ATP, firefly luciferases have been used extensively in biotechnology.
and 29 Related for: Firefly luciferase information
Fireflyluciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme catalyses the oxidation...
different luciferases in a variety of light-emitting reactions. The majority of studied luciferases have been found in animals, including fireflies, and many...
Fireflyluciferase is used in forensics, and the enzyme has medical uses – in particular, for detecting the presence of ATP or magnesium. Fireflies produce...
is the substrate of luciferase (EC 1.13.12.7), which is responsible for the characteristic yellow light emission from many firefly species. As with all...
of toxicity. Fireflyluciferase catalyzes a reaction that produces visible light in the 550 to 575 nm range. A click-beetle luciferase is also available...
the firefly (Photinus pyralis). The Biological Bulletin, 375-393 Keller, G.A.; Gould, S.; Deluca, M.; Subramani, S. (1987). "Fireflyluciferase is targeted...
detection using the fireflyluciferase enzyme. A mixture of three enzymes (DNA polymerase, ATP sulfurylase and fireflyluciferase) and a nucleotide (dNTP)...
luciferase, respectively. Because these are generic names, luciferins and luciferases are often distinguished by the species or group, e.g. firefly luciferin...
organisms which bioluminesce, also known as luciferase enzymes. The three main sources are the North American firefly, the sea pansy (and related marine organisms)...
Photinini are a large tribe of fireflies in the subfamily Lampyrinae. Photinus pyralis is famous in biotechnology for its luciferase gene. This is sometimes...
used morphological term “microbodies”. Later, it was described that fireflyluciferase is targeted to peroxisomes in mammalian cells, allowing the discovery...
and the necessary enzyme luciferase are located in a crystalline structure within rod-like bodies in their photophores. Firefly squid are the only cephalopods...
followed by 3 days of constant light (from 96h). Their genomes carry fireflyluciferase reporter genes driven by the promoter sequences of clock genes. The...
the most effective buffer among the ten tested for ATP assays using fireflyluciferase. Tricine has also been found to be an effective scavenger of hydroxyl...
research that led to the discovery of this consensus observed that when fireflyluciferase was expressed in cultured insect cells it was targeted to the peroxisome...
activity (proof-reading) and luminescence real-time detection using the fireflyluciferase. All the key concepts of sequencing by synthesis were introduced,...
and chemical biology. Because the reporter enzymes themselves (e.g. fireflyluciferase) can be direct targets of small molecules and confound the interpretation...
photocytes in fireflies. A reaction among D-luciferin, luciferase, and ATP has been implicated in the mechanism of light production in firefly photocytes...
"Establishment of a transient transfection system and expression of fireflyluciferase in Entamoeba invadens". Molecular and Biochemical Parasitology. 183...
viability of cells using the assessment of ATP and a method known as "fireflyluciferase". Sodium-potassium ratio: Another kind of assay practices the examination...
biological target of ataluren, it was discovered to bind and stabilize fireflyluciferase, thus explaining the mechanism by which it created a false positive...
produced through its reaction with the naturally occurring enzyme fireflyluciferase using a luminometer. The amount of light produced is directly proportional...
evolution. Firefly luciferin is the luciferin found in many Lampyridae species, such as P. pyralis. It is the substrate of beetle luciferases (EC 1.13.12...
"Molecular basis for the high-affinity binding and stabilization of fireflyluciferase by PTC124". Proceedings of the National Academy of Sciences of the...
"Kinetics of regulated protein-protein interactions revealed with fireflyluciferase complementation imaging in cells and living animals". Proceedings...
'glows' upon activation of the p16INK4a promoter due to insertion of fireflyluciferase in place of the endogenous gene. Use of this system revealed the activation...
Lieb WR, Brick P (1998). "Structural basis for the inhibition of fireflyluciferase by a general anesthetic". Biophysical Journal. 75 (5): 2205–2211....
Global Information