Global InformationElectroelution information
Electroelution is a method used to extract a nucleic acid or a protein sample from an electrophoresis gel by applying a negative current in the plane of the smallest dimension of the gel, drawing the macromolecule to the surface for extraction and subsequent analysis.[2] For example, electroblotting and preparative native PAGE are based upon the same principle.[3][4]
- ^ Kastenholz B, Garfin DE (2010). "Isolation of acidic, basic and neutral metalloproteins by QPNC-PAGE" (PDF). Nature Precedings: 1–4. doi:10.1038/npre.2010.4617.1.
- ^ Seelert H, Krause F (2008). "Preparative isolation of protein complexes and other bioparticles by elution from polyacrylamide gels". Electrophoresis. 29 (12): 2617–36. doi:10.1002/elps.200800061. PMID 18494038. S2CID 35874355.
- ^ Zarzosa-Álvarez, Ana L.; et al. (2010). "Electroeluting DNA Fragments". Journal of Visualized Experiments. 43 (43): 2136. doi:10.3791/2136. PMC 3157863. PMID 20834225.
- ^ Kastenholz, B (2004). "Preparative Native Continuous Polyacrylamide Gel Electrophoresis (PNC‐PAGE): An Efficient Method for Isolating Cadmium Cofactors in Biological Systems". Analytical Letters. 37 (4). Informa UK Limited: 657–665. doi:10.1081/al-120029742. ISSN 0003-2719. S2CID 97636537.