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Edman degradation information


Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide.[1] In this method, the amino-terminal residue is labeled and cleaved from the peptide without disrupting the peptide bonds between other amino acid residues.

  1. ^ Edman, P.; Högfeldt, Erik; Sillén, Lars Gunnar; Kinell, Per-Olof (1950). "Method for determination of the amino acid sequence in peptides". Acta Chem. Scand. 4: 283–293. doi:10.3891/acta.chem.scand.04-0283..

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Edman degradation

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Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide. In this method, the amino-terminal residue is labeled and...

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Protein sequencing

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major direct methods of protein sequencing are mass spectrometry and Edman degradation using a protein sequenator (sequencer). Mass spectrometry methods...

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Pehr Victor Edman

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Victor Edman (14 April 1916 — 19 March 1977) was a Swedish biochemist. He developed a method for sequencing proteins; the Edman degradation. Edman was born...

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Bergmann degradation

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sequencing peptides. The later developed Edman degradation is an improvement upon the Bergmann degradation, instead cleaving the N-terminal amino acid...

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Signal peptide

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determine the amino acid sequence of the N-terminal signal peptide by Edman degradation, a cyclic procedure that cleaves off the amino acids one at a time...

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Edman

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in the American Civil War Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide Edman Island, island near the center...

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Phenyl isothiocyanate

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secondary amines, unlike OPA. It is also known as Edman's reagent and is used in Edman degradation. Commercially available, this compound may be synthesized...

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De novo peptide sequencing

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function of the protein. In the old days, this was accomplished by the Edman degradation procedure. Today, analysis by a tandem mass spectrometer is a more...

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Antibody

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There are a variety of methods used to sequence an antibody including Edman degradation, cDNA, etc.; albeit one of the most common modern uses for peptide/protein...

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Isothiocyanate

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sulforaphane Phenyl isothiocyanate, is used for amino acid sequencing in the Edman degradation. Isothiocyanate and its linkage isomer thiocyanate are ligands in...

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Biopolymer

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determining sequence information. Protein sequence can be determined by Edman degradation, in which the N-terminal residues are hydrolyzed from the chain one...

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PTH

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hormone phenylthiohydantoin, an amino acid derivative formed by the Edman degradation GNU Portable Threads in computing Pass the hash attack in computing...

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List of organic reactions

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reaction Dyotropic reaction E1cB elimination reaction Eder reaction Edman degradation Eglinton reaction Ehrlich–Sachs reaction Einhorn variant Einhorn–Brunner...

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Sequencing

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MicroRNA Sequencing. Methods for performing protein sequencing include: Edman degradation Peptide mass fingerprinting Mass spectrometry Protease digests If...

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Proteomics

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analysis has been Edman degradation (introduced in 1967) where a single peptide is subjected to multiple steps of chemical degradation to resolve its sequence...

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Karolinska Institute

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Bengt I. Samuelsson and John Robert Vane) Pehr Edman (1916–1977), chemist (Med. dr 1946). Cf. Edman degradation Sven Ivar Seldinger (1921–1998), radiologist...

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Exopeptidase

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C-terminal Pro from dipeptides The Proteolysis Map Endopeptidase Edman degradation Dansyl chloride Protease Exopeptidases at the U.S. National Library...

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Protein structure

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protein. The sequence of a protein can be determined by methods such as Edman degradation or tandem mass spectrometry. Often, however, it is read directly from...

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Peptide mass fingerprinting

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of analyzing proteins, peptide mass fingerprinting was developed. Edman degradation was used in protein analysis, and it required almost an hour to analyze...

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Phalloidin

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acid sequence of de-sulfurized phalloidin was elucidated through Edman degradation by Wieland and Schön in 1955. Due to its high affinity for actin,...

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Molecular neuroscience

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separation. The amino acid sequence of the protein was analyzed by Edman degradation and then used to construct a cDNA library which could be used to clone...

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Combinatorial chemistry

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series of their encoding amino acid triplets could be determined by Edman degradation. The important aspect of this kind of encoding was the possibility...

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Proctolin

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in 1975. Proctolin was extracted from 125,000 cockroaches and the Edman degradation was carried out on the sample to determine the amino acid sequence...

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Lund University

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formula. Pehr Edman (1916–1977) was a renowned biochemist who developed a method for sequencing proteins, known as the Edman degradation, and has been...

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List of inventors

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practical incandescent light bulb, etc. Pehr Victor Edman (1916–1977), Sweden – Edman degradation for Protein sequencing Sir Robert Geoffrey Edwards (1925–2013)...

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Beefy meaty peptide

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by Yamasaki and Maekawa, who carried out the experiment using the Edman degradation method for N-terminus sequencing and carboxypeptidase A (Cpase A)...

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