Cryomicroscopy is a technique in which a microscope is equipped in such a fashion that the object intended to be inspected can be cooled to below room temperature. Technically, cryomicroscopy implies compatibility between a cryostat and a microscope. Most cryostats make use of a cryogenic fluid such as liquid helium or liquid nitrogen. There exists two common motivations for performing a cryomicroscopy. One is to improve upon the process of performing a standard microscopy. Cryogenic electron microscopy, for example, enables the studying of proteins with limited radiation damage. In this case, the protein structure may not change with temperature, but the cryogenic environment enables the improvement of the electron microscopy process. Another motivation for performing a cryomicroscopy is to apply the microscopy to a low-temperature phenomenon. A scanning tunnelling microscopy under a cryogenic environment, for example, allows for the studying of superconductivity, which does not exist at room temperature.
Cryomicroscopy is a technique in which a microscope is equipped in such a fashion that the object intended to be inspected can be cooled to below room...
Scanning electron cryomicroscopy (CryoSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron...
Transmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission...
Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the...
cells. cryoET is a specialized application of transmission electron cryomicroscopy (CryoTEM) in which samples are imaged as they are tilted, resulting...
substrate-shuttling mechanism of yeast fatty acid synthase by electron cryomicroscopy". Proceedings of the National Academy of Sciences of the United States...
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Integration into the Spliceosomal U11/U12 di-snRNP as Revealed by Electron Cryomicroscopy". Molecular Cell. 17 (6): 869–883. doi:10.1016/j.molcel.2005.02.016...
severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy". Journal of Virology. 80 (16): 7918–28. doi:10.1128/JVI.00645-06. PMC 1563832...
2004). "Molecular model for a complete clathrin lattice from electron cryomicroscopy". Nature. 432 (7017): 573–579. Bibcode:2004Natur.432..573F. doi:10.1038/nature03079...
2016). "A Reminiscence about Early Times of Vitreous Water in Electron Cryomicroscopy". Biophysical Journal. 110 (4): 756–757. Bibcode:2016BpJ...110..756D...
negatively stained and vitreous ice-embedded transmission electron cryomicroscopy (CryoTEM) samples. Single particle analysis methods are, in general...
orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy". Journal of Molecular Biology. 333 (4): 721–745. doi:10.1016/j.jmb...
of the apoptosome was first revealed at 27 Å resolution by electron cryomicroscopy techniques and has a calculated mass of about 1 MDa (Acehan et al. 2002)...
(Mar 1998). "The 80S rat liver ribosome at 25 A resolution by electron cryomicroscopy and angular reconstitution". Structure. 6 (3): 389–399. doi:10...
containing specimens in amorphous ice is the "holy grail" of biological cryomicroscopy.[citation needed] In practice, it is very difficult to achieve high...
"Backbone structure of the infectious ε15 virus capsid revealed by electron cryomicroscopy". Nature. 451 (7182): 1130–4. Bibcode:2008Natur.451.1130J. doi:10.1038/nature06665...
fewer atoms. One technique used to limit radiation damage is electron cryomicroscopy, in which the samples undergo cryofixation and imaging takes place at...
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