crystal structure of cholesterol oxidase from b.sterolicum
Identifiers
Symbol
Chol_subst-bind
Pfam
PF09129
Pfam clan
CL0277
InterPro
IPR015213
SCOP2
1i19 / SCOPe / SUPFAM
Available protein structures:
Pfam
structures / ECOD
PDB
RCSB PDB; PDBe; PDBj
PDBsum
structure summary
In enzymology, a cholesterol oxidase (EC 1.1.3.6) is an enzyme that catalyzes the chemical reaction
cholesterol + O2 cholest-4-en-3-one + H2O2
Thus, the two substrates of this enzyme are cholesterol and O2, whereas its two products are cholest-4-en-3-one and H2O2.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.
The substrate-binding domain found in some bacterial cholesterol oxidases is composed of an eight-stranded mixed beta-pleated sheet and six alpha-helices. This domain is positioned over the isoalloxazine ring system of the FAD cofactor bound by the FAD-binding domain and forms the roof of the active site cavity, allowing for catalysis of oxidation and isomerisation of cholesterol to cholest-4-en-3-one.[1]
^Coulombe R, Yue KQ, Ghisla S, Vrielink A (August 2001). "Oxygen access to the active site of cholesterol oxidase through a narrow channel is gated by an Arg-Glu pair". J. Biol. Chem. 276 (32): 30435–41. doi:10.1074/jbc.M104103200. PMID 11397813.
and 22 Related for: Cholesterol oxidase information
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Siadat, Seyed-Omid Ranaei (2007-08-31). "Covalent attachment of cholesteroloxidase and horseradish peroxidase on perlite through silanization: Activity...
from the University of London where she worked on the structure of cholesteroloxidase. She also has a Diploma in Crystallography from the Imperial College...
First an esterase converts cholesterol esters into cholesterol and free fatty acid. Then an oxidase oxidizes the cholesterol, producing a H2O2 side-product...
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Ghoshroy, KB (21 Apr 1998). "Assessment of the role of an omega loop of cholesteroloxidase: a truncated loop mutant has altered substrate specificity". Biochemistry...
by cytochrome P450 oxidase enzymes, such as CYP3A4. These reactions introduce oxygen into the steroid ring, allowing the cholesterol to be broken up by...
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Laleh (June 2007). "Cloning and sequencing of partial segment of cholesteroloxidase encoding gene fromStreptomyces luridus". Annals of Microbiology....
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chemistry of histones, protein synthesis, the relationships between cholesterol and hypertension, and creatine's uptake by muscle cells. Daly attended...
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