The ATPase assay is a membrane assay that indirectly measures the activity of efflux transporters. ATP Binding Cassette or efflux transporters mediate the transport of substrates across cell membranes against a concentration gradient. ATP cleavage is tightly linked to substrate translocation, as the energy for the substrate translocation is derived from ATP hydrolysis. ATP hydrolysis yields inorganic phosphate (Pi), which can be measured by a simple colorimetric reaction. The amount of Pi liberated is directly proportional to the activity of the transporter.[1]
^Glavinas H, Méhn D, Jani M, Oosterhuis B, Herédi-Szabó K, Krajcsi P (June 2008). "Utilization of membrane vesicle preparations to study drug-ABC transporter interactions". Expert Opinion on Drug Metabolism & Toxicology. 4 (6): 721–32. doi:10.1517/17425255.4.6.721. PMID 18611113.
The ATPaseassay is a membrane assay that indirectly measures the activity of efflux transporters. ATP Binding Cassette or efflux transporters mediate...
from relatively simple membrane assays. like vesicular transport assay, ATPaseassay to more complex cell based assays up to intricate in vivoJeffrey P...
K+-ATPase in congestive heart failure or arrythmias, which causes an inotropic effect, same as many other digitalis like compounds. The Na+,K+-ATPase creates...
ATP-dependent processes, e.g. ATPases, such as acto-myosin ATPase and calcium ATPase involved in muscle contraction, and sodium/potassium ATPase involved in sodium...
fluorescence-based assays, filtration assays, a scintillation proximity assay, a time resolved fluorescence resonance energy transfer assay, an assay based on flashplate...
cell death. Bafilomycin A1 specifically targets the vacuolar-type H+ -ATPase (V-ATPase) enzyme, a membrane-spanning proton pump that acidifies either the...
inhibiting the Na/K-ATPase, also known as the sodium–potassium ion pump. However, adaptations to the alpha-subunit of the Na+/K+-ATPase via amino acid substitutions...
they are neither cytotoxic nor fluorescent. Blebbistatin inhibits myosin ATPase activity and this way acto-myosin based motility. It binds halfway between...
PfATPase6, is a calcium ATPase gene encoded by the malaria parasite Plasmodium falciparum. The protein is thought to be a P-type ATPase involved in calcium...
correctly—referred to as simply "fiber type", and results from the direct assaying of ATPase activity under various conditions (e.g. pH). Myosin heavy chain staining...
complex (NC) or the T3SS apparatus (T3SA); also called injectisome when the ATPase is excluded; see below). Bacterial proteins that need to be secreted pass...
nerve fibers in skin is prevented by ouabain, a drug that inhibits the Na/K-ATPase of cell membranes. Methylene blue has been used as a placebo; physicians...
respectively), which are relatively comfortable to isolate, manipulate and assay. The smallest known contractile vacuoles belong to Chlamydomonas, with a...
possible only recently through the adaptation of genomic high-throughput assays. The mechanisms governing phenotypic plasticity, or the capacity of a cell...
the cell, the most important of these being the inhibition of the Na-K ATPase. This protein enables the cell to exchange the cations Na+ and K+ between...
invertebrates and sea urchins, the rise in pH to about 7.2–7.6 activates ATPase which leads to a decrease in intracellular potassium, and thus induces membrane...
in bacteria, and is mediated by the TRCF (Mfd) protein. TRCF is an SF2 ATPase that uses ATP hydrolysis to translocate on dsDNA upstream of the transcription...
calcium channel. Furthermore, continued action of the basolateral Na+/K+-ATPase might create an electrical gradient favourable for the reabsorption of divalent...
1016/S0163-4453(05)80037-4. PMID 1602151. Ohta S, Kagawa Y (1986). "Human F1-ATPase: molecular cloning of cDNA for the beta subunit". J. Biochem. 99 (1): 135–41...
endosomes, studies used Bafilomycin A1, which is known to block the v-type H+-ATPases of endosomes. This reduces the acidity in endosomes. The physiological...
member of the genus Aphthovirus in the Picornaviridae family. The plaque assay was developed using poliovirus; the discovery of viral replication in culture...
Glycidamide gives a positive response in the Ames/Salmonella mutagenicity assay, which indicates that it can cause mutations in the DNA. However, "Epidemiologic...
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