Predicted secondary structure and sequence conservation of U5
Identifiers
Symbol
U5
Rfam
RF00020
Other data
RNA type
Gene; snRNA; splicing
Domain(s)
Eukaryota
GO
GO:0000351 GO:0000353 GO:0005682 GO:0046540
SO
SO:0000395
PDB structures
PDBe
U5 snRNA is a small nuclear RNA (snRNA) that participates in RNA splicing as a component of the spliceosome. It forms the U5 snRNP (small nuclear ribonucleoprotein) by associating with several proteins including Prp8 - the largest and most conserved protein in the spliceosome, Brr2 - a helicase required for spliceosome activation, Snu114, and the 7 Sm proteins.[1] U5 snRNA forms a coaxially-stacked series of helices that project into the active site of the spliceosome.[2][3] Loop 1, which caps this series of helices, forms 4-5 base pairs with the 5'-exon during the two chemical reactions of splicing.[4][5] This interaction appears to be especially important during step two of splicing, exon ligation.[6]
^Stevens SW, Barta I, Ge HY, Moore RE, Young MK, Lee TD, Abelson J (November 2001). "Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae". RNA. 7 (11): 1543–53. PMC 1370197. PMID 11720284.
^Nguyen TH, Galej WP, Bai XC, Savva CG, Newman AJ, Scheres SH, Nagai K (July 2015). "The architecture of the spliceosomal U4/U6.U5 tri-snRNP". Nature. 523 (7558): 47–52. Bibcode:2015Natur.523...47N. doi:10.1038/nature14548. PMC 4536768. PMID 26106855.
^Yan C, Hang J, Wan R, Huang M, Wong CC, Shi Y (September 2015). "Structure of a yeast spliceosome at 3.6-angstrom resolution". Science. 349 (6253): 1182–91. Bibcode:2015Sci...349.1182Y. doi:10.1126/science.aac7629. PMID 26292707. S2CID 22194712.
^Newman AJ, Norman C (February 1992). "U5 snRNA interacts with exon sequences at 5' and 3' splice sites". Cell. 68 (4): 743–54. doi:10.1016/0092-8674(92)90149-7. PMID 1739979. S2CID 3328508.
^Sontheimer EJ, Steitz JA (December 1993). "The U5 and U6 small nuclear RNAs as active site components of the spliceosome". Science. 262 (5142): 1989–96. Bibcode:1993Sci...262.1989S. doi:10.1126/science.8266094. PMID 8266094.
^O'Keefe RT, Norman C, Newman AJ (August 1996). "The invariant U5 snRNA loop 1 sequence is dispensable for the first catalytic step of pre-mRNA splicing in yeast". Cell. 86 (4): 679–89. doi:10.1016/s0092-8674(00)80140-3. PMID 8752221. S2CID 17484887.
and 29 Related for: U5 spliceosomal RNA information
U5 snRNA is a small nuclear RNA (snRNA) that participates in RNA splicing as a component of the spliceosome. It forms the U5 snRNP (small nuclear ribonucleoprotein)...
U5 binds exon at 3' splice site, and the 5' site is cleaved, resulting in the formation of the lariat; Complex C* (post-spliceosomal complex) U2/U5/U6...
located near the 5' splice site. U6atac minor spliceosomalRNA Brow DA, Guthrie C (July 1988). "SpliceosomalRNA U6 is remarkably conserved from yeast to mammals"...
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involved in the modification of RNA polymerase II transcribed spliceosomalRNAs U1, U2, U4, U5 and U12. Not all snoRNAs that have been localised to Cajal...
Stark (2006-10-20). "Organization of Core Spliceosomal Components U5 snRNA Loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as Revealed by Electron Cryomicroscopy"...
of U5spliceosomalRNA. scaRNAs are a specific class of small nucleolar RNAs that localise to the Cajal bodies and guide the modification of RNA polymerase...
and U5spliceosomalRNA. scaRNAs are a specific class of small nucleolar RNAs that localise to the Cajal bodies and guide the modification of RNA polymerase...
modification of RNA polymerase II transcribed spliceosomalRNAs U1, U2, U4, U5 and U12. ACA57 belongs to the H/ACA box class of guide RNAs as it has the...
binding site for Sm protein. Sm protein is a shared component of spliceosomal snRNPs. SmY RNAs have been found in nematodes of class Chromadorea, which includes...
modification of RNA polymerase II transcribed spliceosomalRNAs U1, U2, U4, U5 and U12. U100 belongs to the H/ACA box class of guide RNAs as it has the...
motifs are: the C/D and H/ACA boxes of snoRNAs, Sm binding site found in spliceosomalRNAs such as U1, U2, U4, U5, U6, U12 and U3, the Shine-Dalgarno sequence...
(Nov 1998). "The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue...
polymerase II transcribed spliceosomalRNAs U1, U2, U4, U5 and U12. ACA66 (SCARNA20) is a member of the H/ACA box class of snoRNAs that guide the sites of...
catalyses the removal (splicing) of an atypical class of spliceosomal introns (U12-type) from messenger RNAs in some clades of eukaryotes. This process is called...
of this complex, and given the names U1, U2, U4, U5 and U6. Four of these snRNAs (U1, U2, U4 and U5) were found to be tightly bound to several small proteins...
polymerase II transcribed spliceosomalRNAs U1, U2, U4, U5 and U12. ACA68 (SCARNA21) is a member of the H/ACA box class of snoRNAs that guide the sites of...
transcribed spliceosomalRNAs U1, U2, U4, U5 and U12. Kiss AM, Jády BE, Bertrand E, Kiss T (July 2004). "Human box H/ACA pseudouridylation guide RNA machinery"...
modification of RNA polymerase II transcribed spliceosomalRNAs U1, U2, U4, U5 and U12. The complete human U12-22/U4-8 scaRNA is composed of two tandem C/D box domains...
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