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Thermoregulation in animals
Ectotherm
Endotherm
Mesotherm
Poikilotherm
Homeothermy
Heterothermy
Stenotherm
Eurytherm
Thermolabile
Thermostability
Gigantothermy
Kleptothermy
Bradymetabolism
Tachymetabolism
Thermogenesis
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Thermolabile refers to a substance which is subject to, decomposition, or change in response to heat. This term is often used
describe biochemical substances.[1]
For example, many bacterial exotoxins are thermolabile and can be easily inactivated by the application of moderate heat.
Enzymes are also thermolabile and lose their activity when the temperature rises.
Loss of activity in such toxins and enzymes is likely due to change in the three-dimensional structure of the toxin protein during exposure to heat.
In pharmaceutical compounds, heat generated during grinding may lead to degradation of thermolabile compounds.
This is of particular use in testing gene function.[2] This is done by intentionally creating mutants which are thermolabile. Growth below the permissive temperature allows normal protein function, while increasing the temperature above the permissive temperature ablates activity, likely by denaturing the protein.
Thermolabile enzymes are also studied for their applications in DNA replication techniques, such as PCR, where thermostable enzymes are necessary for proper DNA replication. Enzyme function at higher temperatures may be enhanced with trehalose, which opens up the possibility of using normally thermolabile enzymes in DNA replication.[3]
^Tan, G; Chen, M; Foote, C; Tan, C (September 2009). "Temperature-sensitive mutations made easy: generating conditional mutations by using temperature-sensitive inteins that function within different temperature ranges". Genetics. 183 (1): 13–22. doi:10.1534/genetics.109.104794. PMC 2746138. PMID 19596904.
^CARNINCI, PIERO; et al. (January 1998). "Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA". Proceedings of the National Academy of Sciences. 95 (2): 520–524. Bibcode:1998PNAS...95..520C. doi:10.1073/pnas.95.2.520. PMC 18452. PMID 9435224. S2CID 43521603.
Thermolabile refers to a substance which is subject to, decomposition, or change in response to heat. This term is often used describe biochemical substances...
Thermolabile Protecting Groups (TPGs) are applied in chemical synthesis when mild deprotection conditions are required. Their removal merely consists of...
allele (leading to a valine substitution at amino acid 222) encodes a thermolabile alternative enzyme variant with reduced activity. Both reference and...
tyrosinase, an enzyme involved with melanin production. The mutated enzyme is thermolabile; it fails to work at normal body temperatures, but becomes active in...
reaction is increased under acidic conditions. Thiamine is degraded by thermolabile thiaminases present in some species of fish, shellfish and other foods...
Enterobacteriaceae. It is capable of producing enterotoxins which are thermolabile or thermostable. Other characteristics of E. coli are that it is oxidase-negative...
Scalice ER, Christy KG, Atwood SM, Daiss JL (May 1994). "Antibodies as thermolabile switches: high temperature triggering for the polymerase chain reaction"...
Clarification of Valencia orange juice is influenced by specific activity of thermolabile pectinmethylesterase, inactive PME-pectin complexes and the changes in...
Reaction of chlorine trifluoride with nitric acid. Chlorosyl fluoride is thermolabile and disproportionates to ClF and ClO 2F.: 2FClO → ClF + FClO2 2FClO →...
soups, and can be used as pasta fillings. As morels are known to contain thermolabile toxins, they must always be cooked before eating. Morels can be preserved...
R. Araya, N. Timpson, I. N. M. Day & S. Ebrahim (April 2006). "The thermolabile variant of MTHFR is associated with depression in the British Women's...
pressure involved, many supercritical fluid methods can incorporate thermolabile materials. Modern techniques involve renewable, nonflammable and nontoxic...
(PA); the oedema factor (EF); and the lethal factor (LF). Each is a thermolabile protein of ~80kDa. PA forms the "B" part of the exotoxin and allows passage...
the end of the 19th century. He named the two substances luciferase, a thermolabile enzyme, and luciferin, which is preserved by hot water but is present...
Scalice ER, Christy KG, Atwood SM, Daiss JL (May 1994). "Antibodies as thermolabile switches: high temperature triggering for the polymerase chain reaction"...
common genetic cause of hyperhomocysteinemia. The resulting enzyme is thermolabile and in homozygotes, enzymatic activity is depressed to 35% of its usual...
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