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Synchronous culture information


A synchronous or synchronized culture is a microbiological culture or a cell culture that contains cells that are all in the same growth stage.[1][2]

As numerous factors influence the cell cycle (some of them stochastic) normal cultures have cells in all stages of the cell cycle. Obtaining a culture with a unified cell-cycle stage is useful for biological research where a particular stage in the cell cycle is desired (such as the culturing of parasitized cells[3]). Since cells are too small for certain research techniques, a synchronous culture can be treated as a single cell; the number of cells in the culture can be easily estimated, and quantitative experimental results can simply be divided in the number of cells to obtain values that apply to a single cell. Synchronous cultures have been extensively used to address questions regarding cell cycle and growth, and the effects of various factors on these.[4]

  1. ^ LLOYD, D.; et al. (1975). "Synchronous Cultures of Micro-organisms : Large-scale Preparation by Continuous-flow Size Selection" (PDF). Journal of General Microbiology. 88 (1): 153–158. doi:10.1099/00221287-88-1-153. PMID 807677.
  2. ^ Thornton, Maureen; Eward, Kathryn Leigh; Helmstetter, Charles E. (May 2002). "Production of Minimally Disturbed Synchronous Cultures of Hematopoietic Cells" (PDF). BioTechniques. 32 (5): 1098–1105. doi:10.2144/02325rr05. PMID 12019783.
  3. ^ Radfar, Azar; et al. (2009). "Synchronous culture of Plasmodium falciparum at high parasitemia levels". Nature Protocols. 4 (12): 1828–1844. doi:10.1038/nprot.2009.198. PMID 20010926. S2CID 238620.
  4. ^ HARVEY, J. D. (1972). "Synchronous Growth of Cells and the Generation Time Distribution". Journal of General Microbiology. 70 (1): 99–107. doi:10.1099/00221287-70-1-99. PMID 5041269.

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Synchronous culture

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A synchronous or synchronized culture is a microbiological culture or a cell culture that contains cells that are all in the same growth stage. As numerous...

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Microbiological culture

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Linares M, Marín-García P, Puyet A, Diez A, Bautista JM (2009). "Synchronous culture of Plasmodium falciparum at high parasitemia levels". Nat. Protoc...

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Sporopollenin

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Orca

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Orcas of the same sex and age group may engage in physical contact and synchronous surfacing. These behaviours do not occur randomly among individuals in...

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Allomyces macrogynus

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unfavourable. The RS can survive desiccation for years. Study requires synchronous culture in defined media. Allomyces macrogynus is commonly grown in media...

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Andronovo culture

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the interval of the 20th–16th centuries BC...Fyodorovka culture, in general, is synchronous with Alakul..." Diakonoff 1995:473 Dodson, John, et al.,...

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Chernyakhov culture

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development from the Wielbark to Chernyakhov culture, given that the latter stage of the Wielbark culture is synchronous with Chernyakhov, and the two regions...

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API

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would expect. An application programming interface can be synchronous or asynchronous. A synchronous API call is a design pattern where the call site is blocked...

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Archaeological culture

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have radically different material cultures even if in other respects, they are very similar. In the past, such synchronous findings were often interpreted...

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Jinggouzi culture

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Inner Mongolia, the Xijiadian culture and, later, the Jinggouzi culture appeared. These cultures were approximately synchronous to the archaeological periods...

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Menstrual synchrony

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control group in McClintock's 1978 study, the experimental group was more synchronous than the control group but only because the control group was too asynchronous...

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Resonant inductive coupling

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Resonant inductive coupling or magnetic phase synchronous coupling is a phenomenon with inductive coupling in which the coupling becomes stronger when...

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Kama culture

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Kama culture, and V.P. Denisov and L.A. Nagovitchin joined the Kama Neolithic finds with combed ceramics into a single Khutorskoye culture, synchronous with...

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Unix time

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Mills-style Unix clock is implemented with leap second handling not synchronous with the change of the Unix time number. The time number initially decreases...

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Bronze Age

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introduction and development of bronze technology were not universally synchronous. Tin bronze technology requires systematic techniques: tin must be mined...

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Suvorovo culture

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smaller pendants. Archaeologically, Suvorovo is synchronous with the Skelya phase of the Sredny Stog culture and is considered to be part of the Suvorovo-Novodanilovka...

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Synchronicity

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"Mind and earth: Psychic Influence Beneath the Surface". Jung Journal: Culture & Psyche. 3 (2). Taylor & Francis: 15–32. doi:10.1525/jung.2009.3.2.15...

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Prehistory of Transylvania

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main phases: A, B, C, and D), whose beginning is synchronous with the final phase of the Sesklo culture (Greece) occupying Banat and most of Transylvania...

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Hiccup

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Hiccup Other names Singultus, hiccough, synchronous diaphragmatic flutter (SDF) Pronunciation /ˈhɪkəp, -ʌp/ HIK-əp, -⁠up Specialty Otorhinolaryngology ...

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Online school

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are through discussion boards, blogs, and wikis. On the other hand, synchronous online courses happen in real-time. The instructor and students all interact...

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Flywheel

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in structure and installation as the synchronous motor (but it is called synchronous compensator or synchronous condenser in this context). There are...

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Clock

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is available, timekeeping can be maintained very reliably by using a synchronous motor, essentially counting the cycles. The supply current alternates...

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Frisians

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contention. Berglund, Björn E. (2002), "Human impact and climate changes—synchronous events and a causal link?", Quaternary International, vol. 105, Elsevier...

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Solutrean

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system of cave chronology, following the Mousterian, and he considered it synchronous with the third division of the Quaternary period. The era's finds include...

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Hiroshi Tamiya

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working with other Japanese scientists, developed techniques for the synchronous culture of the green algae Chlorella, a model organism used by Otto Heinrich...

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