Predicted secondary structure and sequence conservation of Spot_42
Identifiers
Symbol
Spot_42
Alt. Symbols
Spot42
Rfam
RF00021
Other data
RNA type
Gene; sRNA
Domain(s)
Bacteria
SO
SO:0000389
PDB structures
PDBe
Spot 42 (spf) RNA is a regulatory non-coding bacterial small RNA encoded by the spf (spot forty-two) gene.[1]Spf is found in gammaproteobacteria and the majority of experimental work on Spot42 has been performed in Escherichia coli[2][3] and recently in Aliivibrio salmonicida.[4] In the cell Spot42 plays essential roles as a regulator in carbohydrate metabolism and uptake, and its expression is activated by glucose, and inhibited by the cAMP-CRP complex.[5][6][7][8][9]
The sRNA is transcribed from a separate promoter and binds to messenger RNA targets through imperfect base pairing. The half-life of Spot42 in vivo is 12 to 13 minutes at 37 °C.[5] When grown in media supplemented with glucose, each cell contains 100–200 Spot42 copies.[7] The corresponding level is however reduced 3–4-fold when cells are grown in succinate or when cAMP is added to cells grown in glucose.[8]
^Sahagan BG, Dahlberg JE (July 1979). "A small, unstable RNA molecule of Escherichia coli: spot 42 RNA. I. Nucleotide sequence analysis". J. Mol. Biol. 131 (3): 573–592. doi:10.1016/0022-2836(79)90008-1. PMID 390161.
^Ikemura, Tochimichi; Dahlberg (25 July 1973). "Small ribonucleic acids of Escherichia coli. II. Noncoordinate accumulation during stringent control". The Journal of Biological Chemistry. 248 (14): 5033–5041. doi:10.1016/S0021-9258(19)43667-3. PMID 4577762.
^Ikemura, T; Dahlberg (25 July 1973). "Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis". Journal of Biological Chemistry. 248 (14): 5024–5032. doi:10.1016/S0021-9258(19)43666-1. PMID 4577761.
^Hansen, Geir; Ahmad (24 January 2012). "Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida". BMC Genomics. 13: 37. doi:10.1186/1471-2164-13-37. PMC 3295665. PMID 22272603.
^ abBeisel CL, Storz G (2011). "The Base-Pairing RNA Spot 42 Participates in a Multioutput Feedforward Loop to Help Enact Catabolite Repression in Escherichia coli". Mol Cell. 41 (3): 286–297. doi:10.1016/j.molcel.2010.12.027. PMC 3072601. PMID 21292161.
^Moller, T; Franch T; Udesen C; Gerdes K; Valentin-Hansen P (2002). "Spot 42 RNA mediates discoordinate expression of the E. coli galactose operon". Genes Dev. 16 (13): 1696–1706. doi:10.1101/gad.231702. PMC 186370. PMID 12101127.
^ abSahagan, Barbara; Dahlberg (5 July 1979). "A small, unstable RNA molecule of Escherichia coli: spot 42 RNA. II. Accumulation and distribution". Journal of Molecular Biology. 131 (3): 593–605. doi:10.1016/0022-2836(79)90009-3. PMID 229230.
^ abPolayes DA, Rice PW, Garner MM, Dahlberg JE (July 1988). "Cyclic AMP-cyclic AMP receptor protein as a repressor of transcription of the spf gene of Escherichia coli". J. Bacteriol. 170 (7): 3110–3114. doi:10.1128/jb.170.7.3110-3114.1988. PMC 211256. PMID 2454912.
^El Mouali, Y; Gaviria-Cantin, T; Sánchez-Romero, MA; Gibert, M; Westermann, AJ; Vogel, J; Balsalobre, C (June 2018). "CRP-cAMP mediates silencing of Salmonella virulence at the post-transcriptional level". PLOS Genetics. 14 (6): e1007401. doi:10.1371/journal.pgen.1007401. PMC 5991649. PMID 29879120.
Spot42 (spf) RNA is a regulatory non-coding bacterial small RNA encoded by the spf (spot forty-two) gene. Spf is found in gammaproteobacteria and the...
insulation material Spruce-pine-fir, mixed softwood timber Spot42RNA, a small regulatory RNA found in some Gammaproteobacteria Sender Policy Framework...
glass slides or arrays including "spots" of spatially barcoded oligo(dT) tails to capture the poly-adenylated mRNAs. Besides oligo(dT) tail and spatial...
the main symptom on plants is necrotic spots that appear on the leaves. The INSV virus infects by injecting the RNA the virus contains into the cell which...
dried blood spot specimens for detection of human immunodeficiency virus DNA by polymerase chain reaction". J. Clin. Microbiol. 30 (12): 3039–42. doi:10.1128/jcm...
This list of RNA structure prediction software is a compilation of software tools and web portals used for RNA structure prediction. The single sequence...
can often be seen with the unaided eye as large spots. This stops the infection from spreading. RNA interference is also an effective defence in plants...
reproduction of all known organisms and many viruses. DNA and ribonucleic acid (RNA) are nucleic acids. Alongside proteins, lipids and complex carbohydrates...
Tobacco mosaic virus (TMV) is a positive-sense single-stranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially...
its RNA transcripts. The information content of an organism is recorded in the DNA of its genome and expressed through transcription. Here, mRNA serves...
devour". Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome, and may have structures that are either simple or elaborate. Their...
of naturally occurring RNA and DNA. The same nucleobases can be used to store genetic information and interact with DNA, RNA, or other XNA bases, but...
known as the "magic spot" nucleotides, are alarmones involved in the stringent response in bacteria that cause the inhibition of RNA synthesis when there...
Biology of ETH Zurich, and Charles Weiss mann, who looked for a section of mRNA that could make the protein, and in 1985 found a possible section of DNA;...
neurons. CBs are membrane-less organelles and largely consist of proteins and RNA. They were first reported by Santiago Ramón y Cajal in 1903, who called them...
that when complexed with the target RNA modifies the shape to resemble a tRNA precursor which renders the target RNA susceptible to cleavage by ribonuclease...
genetic material from other species (the genes used are “innate” to potatoes). RNA interference is used to “switch off” genes in this case. Simplot hopes that...
to identify and study the virus. Viral genomes can be based in DNA or RNA. RNA viruses are more time-sensitive for genome sequencing, as they degrade...
Unlike other organisms where RNA editing typically involves guide RNAs, MICOTREM involves the insertional editing of RNA, where specific non-templated...
either single-stranded DNA or RNA that specifically bind to a target ligand or ligands. These single-stranded DNA or RNA are commonly referred to as aptamers...
Global Information